We narrowed to 11,282 results for: Vars;

1. pGCNK58

   Plasmid

   #202461

   Purpose

   Plasmid for the expression of the basic region and leucine zipper domain of the Saccharomyces cerevisiae transcription factor GCN4 in bacterial cells

   Depositor

   Arthur Palmer, Stephen Harrison

   Article

   Weiss et al Nature. 1990 Oct 11;347(6293):575-8. doi

   Insert

   Residues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension (GCN4 Budding Yeast)
   

   Expression

   Bacterial

   Available Since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET41a iGluh

   Plasmid

   #119830

   Purpose

   Production of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluh
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   E25A

   Promoter

   T7

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5.1B-EGFP-DmAGO1

   Plasmid

   #22768

   Depositor

   Elisa Izaurralde

   Article

   Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

   Insert

   Argonaut-1, variant A (AGO1 Fly)
   

   Use

   Drosophila expression

   Tags

   EGFP

   Available Since

   Dec. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-LbCas12a-Plus-mCherry

   Plasmid

   #191230

   Purpose

   To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity

   Depositor

   Zhili Rong

   Article

   Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

   Insert

   LbCas12a-Plus-P2A-mCherry
   

   Use

   CRISPR

   Mutation

   D156R, R883K, R887A

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. K20‐scFv‐pSMBP2

   Plasmid

   #159940

   Purpose

   To generate baculovirus for insect cell expression of anti beta-1 integrin scFv K20

   Depositor

   Sandra Schmid

   Article

   Lakoduk et al Traffic. 2020 Sep;21(9):590-602. doi: 10

   Insert

   anti beta-1 integrin scFv
   

   Tags

   6xHis tag, FLAG tag, MBP fusion, Sortase A recogn…

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3 Flag Jnk1b2

   Plasmid

   #13752

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk1b2 (MAPK8 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

7. 645 pCS2-etv2

   Plasmid

   #49005

   Purpose

   Mammalian Expression vector, In-situ probe template and in vitro transcription vector for zebrafish Etv2

   Depositor

   Nathan Lawson

   Article

   Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

   Insert

   ets variant gene 2 (etsrp Zebrafish)
   

   Use

   Invitro transcription, riboprobe template

   Expression

   Mammalian

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOP459

   Plasmid

   #85933

   Purpose

   Encodes the Anti-Ebola 2G4 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

   Depositor

   Timothy Lu

   Article

   Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

   Insert

   Anti-Ebola 2G4 monoclonal antibody
   

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3 Flag Jnk2b2

   Plasmid

   #13757

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk2b2 (MAPK9 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pFLAG GLTP

    Plasmid

    #170740

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

    Insert

    GLTP (GLTP Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS U6 WASp RNAi

    Plasmid

    #11775

    Depositor

    Jack Strominger

    Article

    Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

    Insert

    WASp RNAi (WAS Human)
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA-Prom1 SV8

    Plasmid

    #184011

    Purpose

    Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286).

    Depositor

    Peter Stoilov

    Article

    Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

    Insert

    Prom1 (Prom1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Wild type cDNA

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOP462

    Plasmid

    #85935

    Purpose

    Encodes the Anti-Ebola 13C6 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

    Depositor

    Timothy Lu

    Article

    Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

    Insert

    Anti-Ebola 13C6 monoclonal antibody
    

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. E. coli 16S toehold switch sensor

    Plasmid

    #110698

    Purpose

    Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    E. coli 16S toehold switch sensor
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. UAS::PTPRFb-DN-GFP

    Plasmid

    #37111

    Depositor

    Alvaro Sagasti

    Article

    Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

    Insert

    UAS::PTPRFb-DN-GFP (ptprfb Zebrafish)
    

    Use

    Tol2 gateway expression vector

    Tags

    EGFP

    Mutation

    Deleted aa1386-1909 corresponding to phosphatase …

    Promoter

    10xUAS

    Available Since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3 Flag Jnk2b1

    Plasmid

    #13756

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk2b1 (MAPK9 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pOPS0253

    Plasmid

    #115505

    Purpose

    Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA.

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Rlv3841pNifH pOGG082, gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. B. fragilis 16S toehold switch sensor

    Plasmid

    #110696

    Purpose

    Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    B. fragilis 16S toehold switch sensor
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLXIN-Ap1g1-FKBP

    Plasmid

    #46946

    Purpose

    Retroviral plasmid for co-expressing siRNA-resistant, FKBP-tagged AP-1 gamma subunit and G418 resistance gene in mammalian cells

    Depositor

    Margaret Robinson

    Article

    Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

    Insert

    Ap1g1-FKBP
    

    Use

    Retroviral

    Tags

    Mainly mouse Ap1g1 cDNA, with a fragment of brain…

    Expression

    Mammalian

    Available Since

    Dec. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. RNA polymerase beta prime subunit FLAG

    Plasmid

    #102337

    Purpose

    Targeting plasmids for replacing the S. elongatus PCC 7942 genomic copy of Synpcc7942_1524 (Beta prime subunit of RNA polymerase) with a C-terminal FLAG tagged variant.

    Depositor

    Erin O'Shea

    Article

    Piechura et al Elife. 2017 Dec 14;6. pii: 32032. doi: 1

    Insert

    RNAP Beta Prime Subunit with C-terminal FLAG TAG
    

    Tags

    3x FLAG with 3X GSGS linker

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pOP461

    Plasmid

    #85934

    Purpose

    Encodes the Anti-Ebola 4G7 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

    Depositor

    Timothy Lu

    Article

    Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

    Insert

    Anti-Ebola 4G7 monoclonal antibody
    

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFX-EGFP-KRasCT

    Plasmid

    #175833

    Purpose

    Express EGFP with plasma membrane-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    EGFP with plasma membrane-targeting sequence
    

    Tags

    C-terminal hypervariable region of K-Ras 4B

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAc5.1B-lambdaN-HA-DmAGO1

    Plasmid

    #22420

    Depositor

    Elisa Izaurralde

    Article

    Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

    Insert

    Argonaut-1, variant A (AGO1 Fly)
    

    Use

    Drosophila expression

    Tags

    lambdaN-HA

    Available Since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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24. bdSUMO-R9-GFP-HiBit

    Plasmid

    #170990

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-R9-muGFP-HiBiT
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. 459 pME-etv2

    Plasmid

    #49002

    Purpose

    full length coding sequence for zebrafish etv2 gene in gateway middle entry vector

    Depositor

    Nathan Lawson

    Article

    Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

    Insert

    ets variant gene 2 (etsrp Zebrafish)
    

    Use

    Gateway middle entry

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET41a iGluf

    Plasmid

    #119833

    Purpose

    Production of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluf
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E25D

    Promoter

    T7

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTEV5_ RpMatB_4

    Plasmid

    #78208

    Purpose

    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - weak binding

    Depositor

    Martin Webb

    Article

    Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

    Insert

    matB (His6 C106A R286C T303A Q457C K488A)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C106A R286C T303A Q457C K488A

    Promoter

    T7

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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28. pTEV5_ RpMatB_3

    Plasmid

    #78207

    Purpose

    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak binding

    Depositor

    Martin Webb

    Article

    Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

    Insert

    matB (His6 C106A T167A R286C Q457C K488A)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C106A T167A R286C Q457C K488A

    Promoter

    T7

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBS U6 WIP RNAi

    Plasmid

    #11774

    Depositor

    Jack Strominger

    Article

    Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

    Insert

    WIP RNAi (WIPF1 Human)
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

30. Split-RESCUE-N

    Plasmid

    #170153

    Purpose

    AAV vector carrying the MCP-RESCUE-DDN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    NES-MCP-RESCUE-DDN
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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