We narrowed to 18,135 results for: URE

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MPRA_minCMV+GFP

Plasmid

#204866

Purpose

Vector containing GFP driven by minimal CMV promoter. This cassette can be cloned into MPRA vectors to serve as a reporter gene.

Depositor

Philipp Maass

Article

Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

Insert

minCMV:GFP

Expression

Bacterial and Mammalian

Promoter

minimal CMV

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTmpl-miniDonor-GFP

Plasmid

#238107

Purpose

Mini donor expressing GFP, used for PCR to generate IVT template.

Depositor

Feng Zhang

Article

Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

Insert

CMV-GFP-syntheticPA flanked by R2Tg UTRs and 28S sequence

Expression

Mammalian

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pFUSE-rIgG-Fc2-MFSD8(L9)-Zeo

Plasmid

#239947

Purpose

Expression of MFSD8 loop 9 tagged with rabbit IgG Fc2

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

Fc2-MFSD8(L9)

Expression

Bacterial and Mammalian

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4.10/RSV_SF3B3-miniXon_Luciferase

Plasmid

#174660

Purpose

Plasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.

Depositor

Beverly L. Davidson

Article

Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

Insert

miniXon cassette

Use

Luciferase

Expression

Mammalian

Mutation

Kozak ATG initiation sequence in middle exon

Promoter

RSV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ND4-DdCBE-right side TALE

Plasmid

#157843

Purpose

expresses right-side TALE–DddAtox half ND4-DdCBE in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

COX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA-N–UGI–ATP5B 3'UTR

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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ND4-DdCBE- left side TALE

Plasmid

#157844

Purpose

expresses left-side TALE–DddAtox half ND4-DdCBE in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

SOD2 MTS–3x-HA–ND4 left tale–G1397 DddA-C–UGI–SOD2 3'UTR

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLV6-mPER2-luc

Plasmid

#212035

Purpose

Expression of murine Per2-luciferase in mammalian cells

Depositor

David Hazlerigg

Article

Appenroth et al J Biol Rhythms. 2024 Dec;39(6):554-567.

Insert

mPer2-luciferase (Per2 Mouse)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-(mcs) human CSF1 full length-IRES-Puro

Plasmid

#86797

Purpose

retroviral expression of CSF-1

Depositor

Martine Roussel, Charles Sherr

Article

Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

Insert

CSF-1 (CSF1 Human)

Use

Retroviral

Tags

IRES-Puro

Expression

Mammalian

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-MFSD6-Puro

Plasmid

#239953

Purpose

Lentiviral vector to generate MFSD6 stable expressing cell line under CMV promoter

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

MFSD6

Use

Lentiviral

Tags

myc-flag

Expression

Mammalian

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-MFSD8-Puro

Plasmid

#239954

Purpose

Lentiviral vector to generate MFSD8 stable expressing cell line under CMV promoter

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

MFSD8

Use

Lentiviral

Tags

myc-flag

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-hCD19

Plasmid

#127889

Purpose

Expresses hCD19

Depositor

Anjana Rao

Article

Chen et al Nature. 2019 Mar;567(7749):530-534. doi:

Insert

hCD19 (CD19 Human)

Use

Retroviral

Tags

no tags

Mutation

none

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

Plasmid

#108871

Purpose

Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

Depositor

Alex Schier

Article

Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

Inserts

5xU6:sgRNA
Cas9-t2A-GFP

Use

CRISPR

Promoter

U6 and hsp70

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pNPK-NLS-Vika

Plasmid

#79969

Purpose

expresses human codon-optimized Vika-recombinase in mammalian cells

Depositor

Frank Buchholz

Article

Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. d

Insert

human codon-optimized Vika sequence, NLS tagged

Tags

NLS

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pGL4.10/RSV_SF3B3-Xon_Luciferase

Plasmid

#174659

Purpose

Plasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.

Depositor

Beverly L. Davidson

Article

Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

Insert

Xon cassette

Use

Luciferase

Expression

Mammalian

Mutation

Kozak ATG initiation sequence in middle exon

Promoter

RSV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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TLCV2-APOBEC1-YTHmut

Plasmid

#178950

Purpose

Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

Depositor

Kate Meyer

Article

Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

Insert

APOBEC1-YTHmut-T2A-GFP

Use

Lentiviral and Synthetic Biology; Inducible

Tags

HA

Expression

Mammalian

Promoter

tight TRE

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-UTX

Plasmid

#24168

Purpose

Mammalian expression of human UTX with HA tag

Depositor

Kristian Helin

Article

Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

Insert

UTX (KDM6A Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry_P2AT2A_GFP-nb-2xKS_FtoG

Plasmid

#238237

Purpose

For overexpression of mCherry_P2AT2A_GFP-nb-2xKS_FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-2xKS_FtoG

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-Actin-7

Plasmid

#56421

Purpose

Localization: Actin, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Actin (ACTB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-Flag-hGSDME

Plasmid

#218939

Purpose

Expression of N-terminal FLAG tagged human gasdermin E

Depositor

Christopher LaRock

Article

LaRock et al Nature. 2022 May;605(7910):527-531. doi:

Insert

human GSDME (GSDME Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLIX401 APE1 WT

Plasmid

#227190

Purpose

Inducible lentiviral expression of WT APE1

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

apurinic/apyrimidinic endonuclease APE1 (APEX1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-mCherry-Stim1

Plasmid

#186620

Purpose

Third generation lentiviral vector expressing Stim1-wt fused to mCherry

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Stromal Interaction Molecule 1 (STIM1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Watermelon backbone

Plasmid

#155258

Purpose

Can be used to generate a Watermelon library

Depositor

Joan Brugge, Aviv Regev

Article

Oren et al Nature. 2021 Aug 11. pii: 10.1038/s41586

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_TNF-SBP-EGFP

Plasmid

#65278

Purpose

synchronize trafficking of TNF from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO-EGFP-G3BP1-S149A

Plasmid

#136004

Purpose

DOX-inducible S149A G3BP1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S149A

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-neo-His-MS2BP-G3BP1-S149A

Plasmid

#136009

Purpose

S149A G3BP1 inserted with MS2BP tagged on the N-terminus to use with the Tethering Assay (MS2)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Tags

MS2BP

Expression

Mammalian

Mutation

S149A

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 wt

Plasmid

#120313

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLIX403 RNaseH1 Catalytic Inactive

Plasmid

#237796

Purpose

Inducible lentiviral expression of RNaseH1 Catalytic Inactive

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

RNaseH1 (RNASEH1 Human)

Expression

Mammalian

Mutation

D210N

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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piggyBAC-H2B FR-MQV

Plasmid

#194079

Purpose

Expresses nuclear-localized FR-MQV in mammalian cells

Depositor

Ralph Jimenez

Article

Mukherjee et al Biochemistry. 2020 Oct 6;59(39):3669-368

Insert

FR-MQV

Tags

Histone H2B

Expression

Mammalian

Mutation

Methionine 42 to Glutamine, Cystiene 159 to Valin…

Promoter

CMV7

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV FLEX Synaptophysin GFP

Plasmid

#137188

Purpose

Cre-dependent expression of synaptophysin-GFP

Depositor

Matthew Nolan

Article

Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

Insert

Synaptophysin GFP (Syp Mouse)

Use

AAV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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BglB in pET29b(+)

Plasmid

#55773

Purpose

Expresses beta-glucosidase

Depositor

Justin Siegel

Article

Carlin et al PLoS One. 2016 Jan 27;11(1):e0147596. do

Insert

Beta-glucosidase B (bglB Synthetic)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 30, 2014

Availability

Academic Institutions and Nonprofits only

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