We narrowed to 81,879 results for: TRI

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tet-pLKO.neo_shGFP

Plasmid

#110470

Purpose

Control, silence GFP gene, doxycycline inducible, neomycin selection

Depositor

Sandra Martha Gomes Dias

Article

Reis et al J Biol Chem. 2019 Jun 14;294(24):9342-93

Insert

GFP

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1/TO (RNA PolIII)

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Foff 2.0-ChR2-EYFP

Plasmid

#137163

Purpose

Intersectional viral expression of ChR2-EYFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-ChR2-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

H134R

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-TM-VC

Plasmid

#135499

Purpose

Mammalian expression of VC-fused and FLAG-tagged TAPBPR with MHC TM

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

TAPBPR (TAPBPL Human)

Tags

Luminal/Extracellular FLAG epitope tag, Signal pe…

Expression

Mammalian

Mutation

switched transmembrane domain with that of MHC-I

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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KIF5A-GFP-SspB

Plasmid

#214401

Purpose

Expresses fusion of Kinesin heavy chain 5A (1-572) with GFP and SspB (micro)

Depositor

Liting Duan

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Apr 2;121

Insert

KIF5A-GFP-SspB (Kif5a Rat, Synthetic)

Tags

KIF5A-GFP-SspB

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-VDAC1 [N152B/23R]

Plasmid

#177502

Purpose

Mammalian Expression Plasmid of anti-VDAC1 (Human). Derived from hybridoma N152B/23.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VDAC1 (Homo sapiens) recombinant mouse monoclonal antibody (VDAC1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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GIV-F1685A FLAG

Plasmid

#65948

Purpose

Expression GIV/Girdin F1685A mutation in Mammalian cell

Depositor

Pradipta Ghosh

Article

Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

Insert

GIV/Girdin (CCDC88A Human)

Tags

3xFlag

Expression

Mammalian

Mutation

Phenylalanine 1685 to Alanine

Promoter

CMV

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-nEF-Coff/Fon-ChR2-mCherry

Plasmid

#137144

Purpose

Intersectional viral expression of ChR2-mCherry in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-ChR2-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

H134R

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Synapsin [L125/129R]

Plasmid

#177472

Purpose

Mammalian Expression Plasmid of anti-Pan-Synapsin (Human). Derived from hybridoma L125/129.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Pan-Synapsin (Homo sapiens) recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 12, 2021

Availability

Academic Institutions and Nonprofits only

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VGlut1 scFv [N28/9]

Plasmid

#190545

Purpose

Mammalian Expression of VGlut1 scFV. Derived from hybridoma N28/9.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

VGlut1 (Rattus norvegicus) recombinant scFV (Slc17a7 Mouse)

Tags

HA, 6xHis, Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Foff 2.0-NpHR3.3-EYFP

Plasmid

#137153

Purpose

Intersectional viral expression of NpHR3.3-EYFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-NpHR3.3-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

W179F

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFP

Plasmid

#137139

Purpose

Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-ChR2(ET/TC)-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E123T, T159C

Promoter

nEF

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3xFLAG-RSL1D1(WT)

Plasmid

#122301

Purpose

expresses RSL1D1 protein with a flag tag

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RSL1D1 (RSL1D1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Foff 2.0-Arch3.3-EYFP

Plasmid

#137149

Purpose

Intersectional viral expression of Arch3.3-EYFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-Arch3.3-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Nav1.7 Na+ channel scFv [N68/6]

Plasmid

#182094

Purpose

Mammalian Expression of Nav1.7 Na+ channel scFv. Derived from hybridoma N68/6.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Nav1.7 Na+ channel (Homo sapiens) (SCN9A Mouse)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-(BsaI)_CBh-UN-Cas9

Plasmid

#135011

Purpose

Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9

Depositor

Yosef Shaul

Article

Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

Insert

humanized S. pyogenes Cas9

Tags

126aa domain from HSV-1 UL12 fused to the N-termi…

Expression

Mammalian

Mutation

126aa domain from HSV-1 UL12 fused to the N-termi…

Promoter

CBh

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX LYCHOS (GPR155) WT-FLAG

Plasmid

#199633

Purpose

Lentiviral construct to stably express LYCHOS (GPR155) WT

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSBbi-RP-CoV2/stSpike-puro

Plasmid

#161794

Purpose

Transposon-based SARS-CoV-2 secretable trimeric Spike stable expression vector for protein production

Depositor

Jorge Luis Arias-Arias

Article

Arias-Arias et al Biotechnol Rep (Amst). 2023 Mar;37:e0078

Insert

SARS-CoV-2 secretable trimeric Spike

Use

Transposon

Tags

6xHis and dTomato

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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AC27 pEGFPN1 anti-AMIGO-1 nanobody

Plasmid

#135226

Purpose

Mammalian expression of anti-AMIGO-1 (Mouse) recombinant llama nanobody for use as an intrabody

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-AMIGO-1 (Mouse) recombinant llama nanobody AC27 (Amigo1 L. glama (llama))

Tags

6xHis-HA-EGFP

Expression

Mammalian

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Parvalbumin [L114/3R]

Plasmid

#177459

Purpose

Mammalian Expression Plasmid of anti-Parvalbumin (Rat). Derived from hybridoma L114/3.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Parvalbumin (Rattus norvegicus) recombinant mouse monoclonal antibody (Pvalb Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Parvalbumin [L114/81R]

Plasmid

#177460

Purpose

Mammalian Expression Plasmid of anti-Parvalbumin (Rat). Derived from hybridoma L114/81.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Parvalbumin (Rattus norvegicus) recombinant mouse monoclonal antibody (Pvalb Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 12, 2021

Availability

Academic Institutions and Nonprofits only

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