We narrowed to 55,065 results for: ran

1. lentiSAM v2 (Puro)

   Plasmid

   #92062

   Purpose

   Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Adam Karpf

   Article

   Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 (sgRNA) and EF1a (dCas9-VP64)

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ErkKTR-irFP

   Plasmid

   #111510

   Purpose

   Reporter of Erk activity

   Depositor

   Jared Toettcher

   Article

   Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

   Insert

   ERK KTR (Mapk1 Mouse)
   

   Use

   Lentiviral

   Tags

   iRFP

   Promoter

   SSFV

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. gRNA NIA TLS2

   Plasmid

   #196981

   Purpose

   Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   AtNIA1 gRNAs fused to TLS2 tags
   

   Expression

   Plant

   Promoter

   pU6-26, pU6-29

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKrox24(2xD-E)DsRed

   Plasmid

   #200111

   Purpose

   Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   modified EGR1 promoter
   

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   2 copies of D-E element, no other promoter elemen…

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPGK-SB13

   Plasmid

   #236078

   Purpose

   SB13 transposase, for use with Sleeping Beauty transponson plasmids

   Depositor

   Masashi Narita

   Article

   Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

   Insert

   SB13 transposase
   

   Use

   Sleeping beauty (sb) transposase plasmid

   Promoter

   PGK

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p(A)n-PAUSE

   Plasmid

   #118068

   Purpose

   Transcription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. His6-pAG-RTase

   Plasmid

   #214932

   Purpose

   Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems

   Depositor

   Chuan He

   Article

   Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s

   Insert

   fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…

   Promoter

   T7 promoter

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG/ATP9A-HA

   Plasmid

   #209213

   Purpose

   Mammalian expression of ATP9A

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

   Insert

   ATP9A (ATP9A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP

   Plasmid

   #113884

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFP

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Promoter

   pHR-CMV-TetO2

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-CMV-MCS-GFP-SV-puro

    Plasmid

    #73582

    Purpose

    Third generation lentiviral vector expressing GFP and puromycin

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP tag

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAS_4xMma PylT sfGPF 150TAG

    Plasmid

    #154766

    Purpose

    plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAG in GFP reporter, U25C in PylT

    Promoter

    EF1

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHTNW

    Plasmid

    #136403

    Purpose

    Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2

    Plasmid

    #113885

    Purpose

    Mammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Twin-Strep

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3 deltaN89 Beta-catenin

    Plasmid

    #19288

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N-terminal truncation, deleted aa 1-89

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pLacO-pEF1α-RFP

    Plasmid

    #179507

    Purpose

    Expresses monomeric RFP in mammalian cells under a EF1α promoter

    Depositor

    Yasushi Hiraoka

    Article

    Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

    Insert

    monomeric RFP
    

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK5-HA-SLC38A9.1

    Plasmid

    #71869

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

17. aeBlue chromoprotein

    Plasmid

    #117846

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, aeBlue
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA-DDX3

    Plasmid

    #44975

    Depositor

    Robin Reed

    Article

    Lee et al Nucleic Acids Res. 2008 Aug;36(14):4708-

    Insert

    DDX3 (DDX3X Human)
    

    Tags

    HA and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. plc242-GFP-WDR24

    Plasmid

    #87050

    Purpose

    mammalian expression of WDR24

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    WDR24 (WDR24 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. tet-pLKO shGATA6 puro

    Plasmid

    #72615

    Purpose

    Expresses an inducible short hairpin targeting human GATA6 sequence

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    short hairpin GATA binding protein 6
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Promoter

    TRE Tight

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge