We narrowed to 23,275 results for: CRISPR

1. qTAG-N-Zeo-miRFP670nano3

   Plasmid

   #207702

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miRFP670nano3 cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. CMV-enAsCas12f

   Plasmid

   #204637

   Purpose

   Expression of enAsCas12f in mammalian cells

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   enAsCas12f
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   D196K, N199K, G276R, N328G, D364R

   Promoter

   CMV

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV/MASC_(GLuc)_TOP1

   Plasmid

   #68439

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ132A

   Plasmid

   #69274

   Purpose

   Golden Gate entry vector; 2nd gRNA under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ133B

   Plasmid

   #69283

   Purpose

   Golden Gate entry vector; 3rd gRNA under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-mScarlet-Zeo

   Plasmid

   #207733

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pU6_(Gluc)_INT(GenPurpClon)

   Plasmid

   #68433

   Purpose

   General Purpose cloning vector for "INT"-like constructs under U6-promoter expression.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct
   

   Use

   CRISPR; Cloning vector for generating int-like co…

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-N-Blast-mScarlet

   Plasmid

   #207678

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mScarlet cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pnos-Cas9-nos

   Plasmid

   #62208

   Purpose

   Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   cas9
   

   Expression

   Insect

   Promoter

   nanos

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPomyces-SaCas9

    Plasmid

    #129553

    Purpose

    Contains codon-optimized SaCas9 and gRNA for streptomyces

    Depositor

    Fong Tian Wong, Mingzi M. Zhang

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    SaCas9
    

    Expression

    Bacterial

    Promoter

    rpsL(XC)-BbsI

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-KO-Puro

    Plasmid

    #227262

    Purpose

    Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJAT31

    Plasmid

    #204291

    Purpose

    For inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-DsRed
    

    Use

    CRISPR

    Promoter

    ie1

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpyB-GFP

    Plasmid

    #220190

    Purpose

    sgRNA expression vector - pBG35 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG35 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG35

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPomyces-FnCpf1

    Plasmid

    #129554

    Purpose

    Contains codon-optimized FnCpf1 and gRNA for streptomyces

    Depositor

    Fong Tian Wong, Mingzi M. Zhang

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    FnCpf1
    

    Expression

    Bacterial

    Promoter

    rpsL(XC)-BbsI

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ133A

    Plasmid

    #69275

    Purpose

    Golden Gate entry vector; 3rd gRNA under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTetO-hOSK-CASH-1

    Plasmid

    #188977

    Purpose

    A CASH-1 GSH targeting, doxycycling inducible humanized Oct4, Sox2, and Klf4 expressing CRISPR/Cas9 donor plasmid.

    Depositor

    Sangdun Choi

    Article

    Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

    Insert

    Doxycycline inducible reprogramming donor cassette
    

    Expression

    Mammalian

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. qTAG-C-moxGFP-Zeo

    Plasmid

    #207732

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pCRISPomyces-Sth1Cas9

    Plasmid

    #129552

    Purpose

    Contains codon-optimized Sth1Cas9 and gRNA for streptomyces

    Depositor

    Fong Tian Wong, Mingzi M. Zhang

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    Sth1Cas9
    

    Expression

    Bacterial

    Promoter

    rpsL(XC)-BbsI

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCE046-SiT-Cas12a-[Repr]

    Plasmid

    #128132

    Purpose

    Express both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcript

    Depositor

    Randall Platt

    Article

    Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

    Insert

    AsCas12a-[Repr]-Triplex
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-KO-Blast

    Plasmid

    #227263

    Purpose

    Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge