We narrowed to 16,155 results for: LEA

Showing: 6721 - 6740 of 16155 results

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Hy_pMT Flag-Hel25E K91N

Plasmid

#110121

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Tags

Flag

Expression

Insect

Mutation

K91N mutation

Promoter

Metallothionein Promoter (pMT)

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E D193E

Plasmid

#110120

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Tags

Flag

Expression

Insect

Mutation

D193E mutation

Promoter

Metallothionein Promoter (pMT)

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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shGLDC-DOX

Plasmid

#72883

Purpose

dox inducible knockdown

Depositor

David Sabatini

Article

Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

Insert

RNAi hairpin against GLDC

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only
ADGRL3-CTF-Flag

Plasmid

#201219

Purpose

pCDNA3.1 with kozak (GCC), ADGRL3 C-terminal fragment and C-terminal Flag

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

Insert

ADGRL3 (Adgrl3 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry

Plasmid

#210504

Purpose

expresses NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry component in mammalian cells

Depositor

Dongmin Lee

Article

Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

Insert

NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMT-flk1-cytoBirA-2A-mCherry_Ras

Plasmid

#80056

Purpose

Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Tags

3x HA

Expression

Bacterial

Promoter

flk1

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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Polymerase expression - pCMV-B103 (LM2907)

Plasmid

#208968

Purpose

Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

B103-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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PD-L1-miSFIT-4x

Plasmid

#124684

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-L1 (CD274 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pTER E2F3shRNA human

Plasmid

#66885

Purpose

Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F3

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F transcription factor 3 (E2F3 Human)

Use

RNAi

Expression

Mammalian

Promoter

H1 Tet

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-mCherry.3xFLAG.NOS1APC20-WPRE

Plasmid

#127866

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1APC20 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

C-terminal 20 amino acids only

Promoter

hSyn

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-31SM-PGK-GFP-Blast

Plasmid

#31516

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-31 (Mir31 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

seed mutant control

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
p3E-DrCav1

Plasmid

#194290

Purpose

Multisite gateway entry clone for expression of codon optimised zebrafish caveolin1 with fusion tag at the N-terminus. Parton lab clone KRT

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

caveolin1 (cav1.S Frog)

Use

Multisite gateway entry vector

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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Nup98-pDONR221

Plasmid

#124241

Purpose

Gateway entry clone of Drosophila Nup98-96 cDNA.

Depositor

Peter Vilmos

Article

Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

Insert

Drosophila Nup98-96 (Nup98-96 Fly)

Expression

Insect

Available Since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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MO70 - Orai1 EC-Flag

Plasmid

#79590

Purpose

Transient expression and retroviral Expression

Depositor

Yousang Gwack

Article

Srikanth et al J Biol Chem. 2010 Feb 12;285(7):5066-75.

Insert

Orai1 (ORAI1 Human)

Use

Retroviral

Tags

FLAG (inserted between TM3 and TM4 of the protein…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only
pMKO.1-shBirc5

Plasmid

#160949

Purpose

Birc5 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Birc5 shRNA (Birc5 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-Ctdnep1_Cter

Plasmid

#196522

Purpose

Entry vector for Gateway with Ctdnep1_Cter

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1-Cter (CTDNEP1 Human)

Expression

Bacterial

Mutation

No transmembrane domain

Promoter

CMV promoter; T7 promoter

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR201-Ctdnep1

Plasmid

#196520

Purpose

Entry vector for Gateway with Ctdnep1

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1-N1 (CTDNEP1 Human)

Expression

Bacterial

Promoter

CMV promoter; T7 promoter

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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HP1-ECFP-SNAP

Plasmid

#187966

Purpose

expresses HP1 with C-terminal tags ECFP and SNAP

Depositor

Markus Wahl

Article

Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

Insert

HP1 (CBX5 Human)

Tags

ECFP-SNAP

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-N-gamma-tubulin (1-333)

Plasmid

#87858

Purpose

Fluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA binding domain and NLS

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

gamma-tubulin 1 (TUBG1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Fragment from aa 1 to 333

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-F-API5

Plasmid

#157662

Purpose

Expresses API5 in mammalian cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Tags

FLAG tag

Expression

Mammalian

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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