We narrowed to 9,184 results for: Pol;

1. pBactin-Akna-mEGFP

   Plasmid

   #196866

   Purpose

   Expression of the centrosomal protein Akna fused to enhanced (E)-GFP

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akna-mEGFP (Akna Mouse)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBactin-td-mCherry-Cdk5rap2

   Plasmid

   #196859

   Purpose

   Expression of Cyclin dependent kinase 5 regulatory subunit-associated protein 2 (Cdk5rap2) fused to tandem (td)-mCherry.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   td-mCherry-Cdk5rap2 (Cdk5rap2 Rat)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BCL6-GFP (1-275AA) in Cilantro

   Plasmid

   #169932

   Purpose

   Overexpression of 1-275AA BCL6-GFP fusion

   Depositor

   Benjamin Ebert

   Article

   Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

   Insert

   BCL6 1-275AA (BCL6 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Mutation

   160R>C

   Available Since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDest-1.7kbfabp6-eGFP-pA-CrymCherry

   Plasmid

   #159088

   Purpose

   LR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA inserts

   Depositor

   John Rawls

   Article

   Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

   Inserts

   1.7kb fabp6 promoter (fabp6 Zebrafish)
   eGFP
   polyA
   

   Use

   Fluorescent reporter

   Promoter

   1.7kb fabp6

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. miR5 Mm ATP13A2

   Plasmid

   #171822

   Purpose

   transfer plasmid for lentiviral vector production with miR for Mm ATP13A2

   Depositor

   Veerle Baekelandt

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   ATP13A2 (Atp13a2 Mouse)
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. miR3 Mm ATP13A2

   Plasmid

   #171771

   Purpose

   transfer plasmid for lentiviral vector production with miR for Mm ATP13A2

   Depositor

   Veerle Baekelandt

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   ATP13A2 (Atp13a2 Mouse)
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDECKO_GFP_AAVS1

   Plasmid

   #141211

   Purpose

   pDECKO_GFP_AAVS1_1801. Targeting, non-essential locus control

   Depositor

   Rory Johnson

   Article

   Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

   Insert

   gRNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDECKO_mCherry_AAVS1_1801

   Plasmid

   #159090

   Purpose

   targeting, non-essential locus control

   Depositor

   Rory Johnson

   Article

   Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

   Insert

   gRNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28_6xHis-PARP1delWGR-L713F

   Plasmid

   #173941

   Purpose

   Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delWGR-L713F (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 521-660; L713F, V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti.PGK.blast-Flag-MFF-S155,172A

    Plasmid

    #74380

    Purpose

    Lentiviral expression vector containing flag tagged S155,172A MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 155 Ala, Ser 172 Ala (Numbering based on MFF …

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pCJ199

    Plasmid

    #162672

    Purpose

    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    Putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. shCCL20

    Plasmid

    #65096

    Purpose

    retroviral expression of CCL20 shRNA

    Depositor

    Pau Sancho-Bru

    Article

    Affo et al Gut. 2014 Nov;63(11):1782-92. doi: 10.11

    Insert

    Ccl20 shRNA (Ccl20 Mouse)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pLenti.PGK.blast-Flag-MFF-S155,172E

    Plasmid

    #74443

    Purpose

    Lentiviral expression vector containing flag tagged S155,172E MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 155 Glu, Ser 172 Glu (Numbering based on MFF …

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pQCXIB Flag MFF S155,172A

    Plasmid

    #74387

    Purpose

    Retroviral expression vector containing Flag tagged S155,172A MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 155 Ala, Ser 172 Ala (Numbering based on MFF …

    Promoter

    CMV

    Available Since

    May 13, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pCJ197

    Plasmid

    #162670

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    S131G; codon optimized for expression in E. coli …

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL3-Enhancer-ZC3H12D_PromoterMUT

    Plasmid

    #153068

    Purpose

    ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    ZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutagenesis to disrupt 3 BHLHE40 binding sites. N…

    Promoter

    None

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLJM1 Flag GFP 4E-BP1 Rheb15 CAAX

    Plasmid

    #112762

    Purpose

    express GFP-4EBP1-Rheb15(CAAX mutation)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-GFP-4EBP1-Rheb15 (CAAX mutant) (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Rheb15(CAAX mutant)

    Mutation

    CAAX mutation to prevent lipid localization

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCJ203

    Plasmid

    #162678

    Purpose

    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    deltaF2-A75; codon optimized for expression in E.…

    Promoter

    T7/lac

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hu-TLR4 299 snp-flag pDEST40

    Plasmid

    #42647

    Purpose

    destination vector for expression of human TLR4 299 SNP in mammalian cells

    Depositor

    Scott Friedman

    Article

    Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

    Insert

    TLR4 299 SNP (TLR4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D299G

    Promoter

    CMV

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pQCXIB Flag MFF S155A

    Plasmid

    #74385

    Purpose

    Retroviral expression vector containing Flag tagged S155A MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 155 Ala (numbering based on MFF isoform 1)

    Promoter

    CMV

    Available Since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only