We narrowed to 8,429 results for: BLI

1. RabV CVS-N2c(deltaG)-EGFP

   Plasmid

   #73461

   Purpose

   Expresses EGFP for monosynaptic circuit mapping

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   EGFP
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

   Plasmid

   #66707

   Purpose

   expresses a single gRNA and VP64-dCas9-VP64

   Depositor

   Charles Gersbach

   Inserts

   hU6-gRNA expression cassette
   S.Pyogenes VP64-dCas9-VP64
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   VP64

   Expression

   Mutation

   D10A and H840A

   Promoter

   hU6 and hUbC

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET29b Tau Cys

   Plasmid

   #108867

   Purpose

   Expresses human Tau with a mutated cysteine

   Depositor

   Gabriele Kaminski Schierle

   Insert

   Tau (MAPT Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   I260C C291A C322A to allow maleimide dye labelling

   Promoter

   T7

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CaMKIIa-hM4D(Gi)-mCherry

   Plasmid

   #50477

   Purpose

   Gi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoter

   Depositor

   Bryan Roth

   Has Service

   AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM4D(Gi)-mCherry (CHRM4 Human)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   CaMKIIa

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. Mouse 3' Hp1a AID GFP PuroR

   Plasmid

   #127897

   Purpose

   HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Hp1a Gene

   Depositor

   Mark Groudine

   Insert

   Hp1a (Cbx5 Mouse, Mustard Weed)
   

   Use

   Donor plasmid

   Tags

   GFP, AID, PuroR

   Expression

   Mutation

   Inserting GFP AID 2A Puro into mouse 3' Hp1a

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRN3P_membrane_EGFP

   Plasmid

   #139402

   Purpose

   in vitro transcription for mRNA

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Borsos et al Nature. 2019 May;569(7758):729-733. doi:

   Insert

   membrane-EGFP
   

   Use

   In vitro transcription

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   T3

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-UBC9-K14R-A129K

   Plasmid

   #228948

   Purpose

   express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)

   Depositor

   Marcin Suskiewicz

   Insert

   UBC9 (UBE2I Human)
   

   Use

   Tags

   His tag and thrombine cleavage site : MGHHHHHHSSG…

   Expression

   Bacterial

   Mutation

   changed Lysine 14 to Arginine, changed Alanine 12…

   Promoter

   T7-lac

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLT3-D835Y-MSCV-IRES-GFP

   Plasmid

   #184780

   Purpose

   Expresses FLT3 D835Y activating tyrosine kinase domain mutant in mammalian cells

   Depositor

   Timothy Ley

   Insert

   FLT3 (FLT3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Activating tyrosine kinase domain mutation (D835Y)

   Promoter

   MSCV

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMC037-HisMS2_PLP_pac

   Plasmid

   #128233

   Purpose

   Expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

   Depositor

   Paul Freemont

   Article

   de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

   Inserts

   Maturation Protein
   Coat Protein Dimer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 15, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pEC3105 (pSpy1C-PxylS2_ffluc)

    Plasmid

    #218512

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    deletion of Plac_mrfp cassette

    Promoter

    PxylS2

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPGKpuro

    Plasmid

    #11349

    Depositor

    Rudolf Jaenisch

    Article

    Tucker et al Genes Dev. 1996 Apr 15. 10(8):1008-20.

    Insert

    pgk-1 5' and 3' regions (Pgk1 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-osTIR1-9xMyc-Zeo

    Plasmid

    #225696

    Purpose

    Lentiviral expression of 9xMycTag fused osTIR1and Zeocin resistance. For use with AID degron.

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    osTIR1
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    9X Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Membrane mNeonGreen

    Plasmid

    #198057

    Purpose

    Plasma membrane localizing mNeonGreen using Human LCK proto-oncogene sequence

    Depositor

    Amro Hamdoun

    Insert

    LCK membrane mNeonGreen (LCK Human, A.victoria)
    

    Use

    Synthetic Biology; Sea urchin, mammal, zebrafish

    Tags

    mNeonGreen

    Expression

    Mutation

    Tandem repeat of N-terminal LCK membrane targetin…

    Promoter

    sp6

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEC2861 (pSpy1C-PgyrA_ffluc)

    Plasmid

    #218511

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    deletion of Plac_mrfp cassette

    Promoter

    PgyrA

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE2-EF1a-HA-ATAD3A-IRES-Hygro

    Plasmid

    #211469

    Purpose

    Lentivirus backbone expressing ATAD3A with an HA-tag at the N-terminal

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ATAD3A (ATAD3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDB179-DepoPETase

    Plasmid

    #218702

    Purpose

    Encodes codon optimized DepoPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    DepoPETase
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. RabV CVS-N2c(deltaG)-tdTomato

    Plasmid

    #73462

    Purpose

    Expresses tdTomato for monosynaptic circuit mapping

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    tdTomato
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pdCas9-NED

    Plasmid

    #109358

    Purpose

    Mammalian vector for expressing effector domains fused to dCas9.

    Depositor

    Antal Kiss

    Article

    Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    dCas9, SV40 NLS, 3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. HaloTag-A2A-adenosine receptor

    Plasmid

    #66995

    Purpose

    A2A-adenosine receptor tagged with HaloTag on amino terminus

    Depositor

    Catherine Berlot

    Insert

    HaloTag-A2A-adenosine receptor (ADORA2A Human)
    

    Use

    Tags

    Human Growth hormone Signal Sequence- HaloTag

    Expression

    Mammalian

    Mutation

    The signal sequence from human growth hormone was…

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro

    Plasmid

    #211468

    Purpose

    Lentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminal

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    4xGFP11-SEC61B
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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