We narrowed to 23,275 results for: CRISPR

1. pYPQ132-tRNA

   Plasmid

   #179211

   Purpose

   Golden Gate entry vector; 2nd gRNA with tRNA

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Promoter

   no promoter

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. CL7-LbCas12a

   Plasmid

   #164658

   Purpose

   Bacterial expression of LbCas12a using CL7/Im7

   Depositor

   Piyush Jain

   Article

   Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

   Insert

   LbCas12a
   

   Tags

   Thioredoxin, CL7, SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWalium20-10XUAS-3XFLAG-dCas9-VPR

   Plasmid

   #78897

   Purpose

   Expresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for in vivo CRISPRa

   Depositor

   Norbert Perrimon

   Article

   Lin et al Genetics. 2015 Oct;201(2):433-42. doi: 1

   Insert

   dCas9-VPR
   

   Tags

   3xFLAG

   Expression

   Insect

   Promoter

   10XUAS

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-N-Blast-3xFLAG

   Plasmid

   #207684

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xFLAG cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. Spacer Switch Plasmid

   Plasmid

   #215346

   Purpose

   Facilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.

   Depositor

   Hank Seifert

   Article

   Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

   Insert

   OpaD spacer; Chloramphenicol Resistance Cassette
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Level 1 P4 TaU6 guide acceptor

   Plasmid

   #165600

   Purpose

   GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid

   Depositor

   Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

   Article

   Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

   Insert

   TaU6 promoter::LacZ::sgRNA scaffold
   

   Use

   CRISPR and Synthetic Biology; Sgrna acceptor with…

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Level 1 P3 TaU6 guide acceptor

   Plasmid

   #165599

   Purpose

   GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid

   Depositor

   Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

   Article

   Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

   Insert

   TaU6 promoter::LacZ::sgRNA scaffold
   

   Use

   CRISPR and Synthetic Biology; Sgrna acceptor with…

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-N-Blast-miniTurbo

   Plasmid

   #207681

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miniTurbo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. qTAG-C-sTagRFP-Puro

   Plasmid

   #207723

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pLBH545_Tet-Cas14a1_Locus

    Plasmid

    #112501

    Purpose

    Expresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-N-Puro-miniTurbo

    Plasmid

    #207692

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-miniTurbo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. qTAG-C-3xHA-Blast

    Plasmid

    #207718

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. qTAG-N-Blast-V5

    Plasmid

    #207686

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-V5 cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pSF279

    Plasmid

    #167674

    Purpose

    Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-N-Zeo-3xFLAG

    Plasmid

    #207706

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xFLAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. qTAG-N-Blast-3xHA

    Plasmid

    #207685

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xHA cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pN-PITCh-GFP

    Plasmid

    #127888

    Purpose

    Provides the repair template with PSMD2 microhomology on each end for PSMD2 N-ter GFP tagging

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Insert

    Puro-GFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC-U63-gRNA2.1

    Plasmid

    #170512

    Purpose

    gRNA cloning vector containing a U6:3 promoter and a gRNA2.1 scaffold.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6:3

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. qTAG-C-moxGFP-Puro

    Plasmid

    #207721

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. Brp-T2A-QF2w-Hsp70-3XP3-dsRed

    Plasmid

    #141094

    Purpose

    Template plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.

    Depositor

    Carolyn McBride

    Article

    Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

    Insert

    bruchpilot
    

    Use

    CRISPR

    Expression

    Insect

    Mutation

    coding sequence downstream of CRISPR cut site, sy…

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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