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pcDNA_DiCre_59.F2

Plasmid

#60207

Purpose

Expresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)

Depositor

Jean-Paul Herman

Article

Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

Insert

Cre recombinase (CD207 Bacteriophage P1, Human, Synthetic)

Tags

FKBP12 and Nuclear localisation signal

Expression

Mammalian

Mutation

amino acids 19-59 of the humanized version of Cre…

Promoter

CMV

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA4-ATR-C-KD

Plasmid

#53770

Purpose

mammalian expression of flag-tagged ATR C-terminal fragment kinase dead mutant

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

Insert

ATR (ATR Human)

Tags

Flag

Expression

Mammalian

Mutation

C-terminal domain, aa 1398-2644, and kinase dead …

Promoter

CMV

Available Since

July 8, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-KLF3

Plasmid

#49100

Purpose

Expresses KLF3 in mammalian cells

Depositor

Merlin Crossley

Article

Bell-Anderson et al Diabetes. 2013 Aug;62(8):2728-37. doi: 1

Insert

Krüppel-Like Factor 3(KLF3)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only
pMT-mCherry-Patronin

Plasmid

#31753

Depositor

Ron Vale

Article

Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

Insert

Patronin (Patronin Fly)

Tags

mCherry

Expression

Insect

Promoter

Metallothionein

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pBabe EGFR Del2

Plasmid

#32065

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Delete S752-I759

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pCold TDP43 G294A

Plasmid

#27452

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

Factor Xa and His6

Expression

Bacterial

Mutation

Glycine 294 mutated to Alanine (G294A)

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pGL3E-hIL-17prom(-232)-Luc

Plasmid

#25628

Depositor

Sarah Gaffen

Article

Liu et al J Biol Chem. 2004 Dec 10. 279(50):52762-

Insert

human IL-17 promoter (IL17A Human)

Use

Luciferase

Available Since

June 25, 2010

Availability

Academic Institutions and Nonprofits only
PKC beta I dNPS

Plasmid

#16379

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta 1 (Prkcb Rat)

Tags

HA

Expression

Mammalian

Mutation

N-terminal pseudosubstrate domain deletion: aa 30…

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pMKO shRNA Bad

Plasmid

#17237

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bad shRNA (BAD Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pBS mouse c-Myc

Plasmid

#14972

Depositor

Joan Massague

Article

Siegel et al J Biol Chem. 2003 Sep 12. 278(37):35444-

Insert

c-Myc (Myc Mouse)

Expression

Bacterial

Mutation

Nucleotides 2578-3294

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
p206-Switch-ON

Plasmid

#217885

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Retroviral

Expression

Mammalian

Promoter

hU6

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; gcry1:BFP -2

Plasmid

#173888

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; gcry1:BFP -0

Plasmid

#173886

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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dCas9-MSK1

Plasmid

#165601

Purpose

Expresses Sp dCas9 fused to human MSK1

Depositor

Isaac Hilton

Article

Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

Insert

ribosomal protein S6 kinase A5 (RPS6KA5 Human, S. Pyogenes, Synthetic)

Use

CRISPR and Lentiviral

Tags

FLAG Tag

Expression

Mammalian

Promoter

EF1a

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-RAB3C-IRES-Puromycin

Plasmid

#134857

Purpose

Expresses EGFP-tagged synaptic vesicle marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Ras-related protein 3C

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Epac1 CAAX

Plasmid

#118312

Purpose

Expression of human Epac1

Depositor

Philip Stork

Article

Wang et al Mol Cell Biol. 2006 Mar;26(6):2130-45. d

Insert

Epac1 CAAX (RAPGEF3 Human)

Tags

GFP

Expression

Mammalian

Mutation

C-terminal 20aa of KiRas

Promoter

CMV

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEBB HA cIAP1 H588A

Plasmid

#38233

Depositor

Colin Duckett

Article

Csomos et al Biochem J. 2009 Apr 28;420(1):83-91.

Insert

cIAP1 H588A (BIRC2 Human)

Tags

HA

Expression

Mammalian

Mutation

H588A

Promoter

EF1a

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
pSicoR-Ef1a-mCh-Puro-Luci

Plasmid

#31850

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

Luciferase RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pR-BAM

Plasmid

#22649

Depositor

Lynn Hudson

Article

Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

Insert

Myelin Transcription Factor 1L (Myt1l Rat)

Use

Cloning and rna synthesis

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pEBB-II-I-GFP gamma isoform

Plasmid

#22148

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only