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We narrowed to 18,247 results for: multi

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Showing: 6741 - 6760 of 18247 results

  4. pCRISPRi

    Plasmid
    #199808
    Purpose
    vector for constitutive CRISPRi-mediated gene knockdown in S. aureus
    Depositor
    Steve Salipante
    Publication
    Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9
    sgRNA with GFP cassette
    Use
    CRISPR
    Promoter
    P23 and PRAB17 (sgRNA) and SarAP1 (GFP)
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    199808-plasmid-map-sequence-id-403016

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  8. pJH130

    Plasmid
    #162484
    Purpose
    SEC plasmid containing LG1 homology arms and myo-3p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.
    Depositor
    Bob Goldstein
    Publication
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    myo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Use
    CRISPR
    Tags
    myo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Expression
    Worm
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    162484-plasmid-map-sequence-id-320834

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  11. pDB038b

    Plasmid
    #99212
    Purpose
    Accessory plasmid for PACE of PylRS variants
    Depositor
    David Liu
    Publication
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII(P29*,P83*,Y184*), luxAB; PproK pylT
    Expression
    Bacterial
    Mutation
    amber codons at positions 29, 83, and 184 of gIII
    Promoter
    Ppsp and PproK
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99212-plasmid-map-sequence-id-192366

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  12. pGAPTrap-mCherry-IRESMygro

    Plasmid
    #82505
    Purpose
    Vector targeting the mCherry gene to the GAPDH locus of human cells. mCherry is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.
    Depositor
    Ed Stanley
    Publication
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-IRESMygro
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    82505-plasmid-map-sequence-id-156572

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  14. MSP2266

    Plasmid
    #70705
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70705-plasmid-map-sequence-id-131909

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  16. ADORA2A-DuET

    Plasmid
    #213174
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    ADORA2A (ADORA2A Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213174-plasmid-map-sequence-id-430742

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  17. CROPseq-Guide-BSD

    Plasmid
    #216123
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the blasticidin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Stefan Kubicek
    Publication
    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216123-plasmid-map-sequence-id-428727

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  18. pBBR1.cas9-NT

    Plasmid
    #197871
    Purpose
    Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)
    Depositor
    Baojun Wang
    Publication
    Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72
    Inserts
    trancCRNA
    Cas9
    crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
    Expression
    Bacterial
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197871-plasmid-map-sequence-id-396456

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Showing: 6741 - 6760 of 18247 results