We narrowed to 12,039 results for: SHA;

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pC010 LshCas13a locus with nontargeting spacer

Plasmid

#91901

Purpose

LshCas13a locus into pACYC184 with nontargeting spacer

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LshCas13a locus with non-targeting spacer

Expression

Bacterial

Available Since

Oct. 16, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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PPAPDC2

Plasmid

#155836

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PPAPDC2 (PLPP6 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis(TEV)-dDrp1-StrepII

Plasmid

#174421

Purpose

Expresses D. melanogaster Drp1 in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1 (Drp1 Fly)

Tags

6xHis(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-vCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

Plasmid

#197966

Purpose

T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by ribozymes.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pco-vCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

CmYLCVp and pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-Gαi3-YFP (Citrine variant, b/c loop insert)

Plasmid

#171375

Purpose

For the mammalian expression of rat Gαi3 internally tagged with YFP

Depositor

Mikel Garcia-Marcos

Article

Marivin et al Sci Signal. 2016 Apr 12;9(423):ra37. doi

Insert

Gai3-YFP

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pFLAG W96A-GLTP

Plasmid

#170741

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

Insert

GLTP (GLTP Human)

Tags

FLAG

Expression

Mammalian

Mutation

W96A

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJL1-mRFP1 gRNA

Plasmid

#117052

Purpose

In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

anti-mRFP1 SpCas9 gRNA

Expression

Bacterial

Promoter

T7

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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OA-1045C

Plasmid

#125005

Purpose

Expresses gRNAs targeting hid, eve, and wingless

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

eve gRNA (eve Fly)

Expression

Insect

Promoter

U6:3

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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mRuby2-H2B-6

Plasmid

#55897

Purpose

Localization: Nucleus/Histones, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

H2B (H2BC11 Human)

Tags

mRuby2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pMT2 TIA1 RRM-CFP-FRBx5

Plasmid

#103783

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

TIA1 RRM-CFP-FRBx5

Tags

CFP

Expression

Mammalian

Promoter

SV40

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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YFP-Rpp25-C1

Plasmid

#134544

Purpose

Expresses YFP-Rpp25 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Rpp25 (RPP25 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLVE-FH-IN

Plasmid

#70071

Purpose

2nd gen lentiviral vector. Cre-removable and tet/dox controllable expression of FLAG-HA-tagged transgene in mammalian cells

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Type

Empty backbone

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr-D435A/E437A

Plasmid

#40829

Purpose

Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

Depositor

Phillip Zamore

Article

Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

Insert

Nbr D435A/E437A (Nbr Fly)

Tags

3xFLAG and 6xMyc

Expression

Insect

Mutation

D435A/E437A, part of the DEDD motif; mutations p…

Promoter

Actin5C

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pUAST-SIK3 T196E

Plasmid

#52975

Purpose

insect expression of SIK3 T196E constitutively active mutant

Depositor

Marc Montminy, Biao Wang

Article

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Tags

HA

Expression

Insect

Mutation

T196E constitutively active mutant

Promoter

Hsp70

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
pEJS482-pCDest2-AcrIIC3Nme-mTagBFP2-IRES

Plasmid

#85750

Purpose

Expresses Type II-C anti-CRISPR protein, AcrIIC3Nme from Neisseria meningitidis, and mTagBFP2

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Type II-C anti-CRISPR AcrIIC3Nme
mTagBFP2

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pMGIB-HA-hTCAB1

Plasmid

#167460

Purpose

Retroviral expression of HA-TCAB1 (WDR79) in mammalian cells

Depositor

Steven Artandi

Article

Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

Insert

TCAB1 (WRAP53 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR3-deGFP-6xHis-T500

Plasmid

#92224

Purpose

Expression of deGFP using the p70a promoter and UTR3

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

deGFP

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70a

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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MMTV-VEGF

Plasmid

#44625

Depositor

Robert Oshima

Article

Oshima et al Cancer Res. 2004 Jan 1;64(1):169-79.

Insert

VEGF121 (Vegfa Mouse)

Use

Mouse Targeting

Expression

Mammalian

Mutation

contains aa160-368; G165E mutation

Promoter

Mouse mammary tumor virus promoter

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
pLXSN-FLK1 TM

Plasmid

#65249

Purpose

Expression of dominant negative FLK1 in mammalian cells

Depositor

Axel Ullrich

Article

Millauer et al Nature. 1994 Feb 10;367(6463):576-9.

Insert

FLK1 TM (Kdr Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

deleted AA 786-1346

Promoter

LTR

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pLN193 (SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe))

Plasmid

#105210

Purpose

Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 3 intron - see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe)

Use

Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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