We narrowed to 12,216 results for: SHA;

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pcDNA SF-HERC2 F2

Plasmid

#55785

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2 (950-1750aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (950aa-1750aa) (HERC2 Human)

Tags

3xFLAG and STREP

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
Nrxn3α-AS4-

Plasmid

#184332

Purpose

Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

Depositor

Peter Scheiffele

Article

Nguyen et al Elife. 2016 Dec 13;5. doi: 10.7554/eLife

Insert

Nrxn3-alpha (Nrxn3 Mouse)

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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loSIIN

Plasmid

#185663

Purpose

shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc
GFP

Use

Lentiviral

Promoter

Ef1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAM4941 (pCV0053)

Plasmid

#120083

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 3 (NS3).

Depositor

Susan Golden

Article

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS3-RK2BOM, CV-aacC1, CV-ccdB-SwaI

Use

Other

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1

Plasmid

#39281

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus with a 4bp deletion (TA…

Available Since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 6B CARD8 UPA-CARD-mCherry R464E

Plasmid

#164013

Purpose

Mammalian expression vector encoding CARD8 UPA-CARD (filament-deficient mutation) with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

mCherry

Expression

Mammalian

Mutation

R464E

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P81nmt1

Plasmid

#39282

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus with a 7bp deletion (AT…

Available Since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pDONR221 DPP9S

Plasmid

#166789

Purpose

pDONR gateway shuttling vector with DPP9S (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Gateway shuttling vector

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pHHM.X513-iNicSnFR3b-(V7)

Plasmid

#124881

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

Depositor

Henry Lester

Article

Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

Insert

iNicSnFR3b-(V7)

Tags

HA Tag, His Tag, and NA

Expression

Bacterial

Mutation

NA

Promoter

T7

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3- myc NES-mCE(K294A) NLS

Plasmid

#82467

Purpose

Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NLS deleted from mouse Capping Enzyme (Rngtt Mouse)

Expression

Mammalian

Mutation

deletion of KRKY from 573-657

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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CamKIIa:smFP-TVA-RGV

Plasmid

#202594

Purpose

Expresses TVA receptor, rabies oG and “spaghetti monster” fluorescent protein in mature excitatory neurons.

Depositor

Oleksandr Shcheglovitov

Article

Chiola et al Mol Psychiatry. 2021 Feb 8. pii: 10.1038

Inserts

smFP
TVA
RGV

Use

Lentiviral

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-NR4A3

Plasmid

#139818

Purpose

doxycycline-inducible expression of Human NR4A3 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

NR4A3 (NR4A3 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S240G- please see depositor comment below

Promoter

TRE-mCMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pEVOL-AcKRS-CloDF

Plasmid

#127415

Purpose

Mutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophage

Depositor

Wenshe Liu

Article

Wang et al Angew Chem Int Ed Engl. 2019 Oct 28;58(4

Insert

Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair

Expression

Bacterial

Promoter

araBAD

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-ITR-PytR-mCherry

Plasmid

#204868

Purpose

Expresses pyrrolysyl tRNA and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

M. mazei pyrrolysyl tRNA

Use

AAV

Expression

Mammalian

Mutation

U25C

Promoter

U6

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221 DPP9L

Plasmid

#166790

Purpose

pDONR gateway shuttling vector with DPP9L (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Gateway shuttling vector

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-ITR-EcYtR-mCherry

Plasmid

#217362

Purpose

Expresses E. coli tyrosine tRNA and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

E. coli tyrosine tRNA

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B

Plasmid

#136012

Purpose

EIF3B 3'UTR (NM_001037283.1) inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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RS35075_pMQ30_KO_Kan

Plasmid

#185390

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS35075 upstream flanking homology region
RR42_RS35075 downstream flanking homology region
nptII kanamycin resistance marker

Expression

Bacterial

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pEF1α:flex-mcherry

Plasmid

#222618

Purpose

Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

Depositor

Franck Polleux

Article

Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

Insert

Cre-dependent mCherry

Use

AAV

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBLO ddd64.1

Plasmid

#123121

Purpose

E. coli expression vector for catalytically inactivated deltaproteobacteria CasX

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

CasX

Use

CRISPR

Mutation

Mutated amino acids in catalytic triad to alanine…

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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