We narrowed to 22,314 results for: CRISPR

1. pJC005.em

   Plasmid

   #182738

   Purpose

   CRISPR-Cas12a genome editing of E. faecium

   Depositor

   James Collins

   Article

   Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEG302-SunTag-FWAgRNA-4-22aa-TET1cd

   Plasmid

   #115344

   Purpose

   SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

   Depositor

   Steven Jacobsen

   Insert

   gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJC005.gent

   Plasmid

   #182739

   Purpose

   CRISPR-Cas12a genome editing of E. faecium

   Depositor

   James Collins

   Article

   Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKIR1.1

   Plasmid

   #85758

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Plant

   Mutation

   Promoter

   AtRPS5A

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDCC6

   Plasmid

   #59985

   Purpose

   Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

   Depositor

   Peter Duchek

   Article

   Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

   Inserts

   Cas9
   U6-2>sgRNA
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   U6-96Ab and hsp70Bb

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMZ374

   Plasmid

   #59896

   Purpose

   Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).

   Depositor

   Mikel Zaratiegui

   Article

   Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

   Inserts

   Cas9
   rrk1:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Silent muation of the CspCI site

   Promoter

   adh1 and rrk1

   Available since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYZ033

   Plasmid

   #98404

   Purpose

   Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDCC5

   Plasmid

   #59984

   Purpose

   Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

   Depositor

   Peter Duchek

   Article

   Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Insect

   Mutation

   D10A

   Promoter

   hsp70Bb

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAH237

   Plasmid

   #121146

   Purpose

   Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast

   Depositor

   Katsunori Tanaka

   Article

   Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

   Inserts

   gRNA cassette
   humanized Streptococcus pyogenes Cas9
   

   Use

   CRISPR

   Tags

   Flag

   Expression

   Yeast

   Mutation

   Promoter

   S.pombe rrk1 and nmt41

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI5-CasRx-NLS-HA (pKW919)

    Plasmid

    #229047

    Purpose

    Mammalian expression of CasRx-NLS-HA

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Insert

    CasRx (RfxCas13d)
    

    Use

    CRISPR

    Tags

    NLS and NLS-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI5-CasRx-NES-HA (pKW387)

    Plasmid

    #229048

    Purpose

    Mammalian expression of CasRx-NES-HA

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Insert

    CasRx (RfxCas13d)
    

    Use

    CRISPR

    Tags

    NES-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI5-CasRx-HA (pKW88)

    Plasmid

    #229046

    Purpose

    Mammalian expression of CasRx-HA

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Insert

    CasRx (RfxCas13d)
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pST_116_LVL2 cam

    Plasmid

    #179332

    Purpose

    NT-CRISPR plasmid for a single gRNA.

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSCIP-mB2M

    Plasmid

    #154091

    Purpose

    Self-Cutting and Integrating CRISPR backbone targeting murine B2M

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Insert

    sgRNA and BAIT targeting murine B2M locus
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pST_119_LVL2 cam

    Plasmid

    #179333

    Purpose

    NT-CRISPR plasmid for integration of multiple gRNAs.

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPbU6_hdhfr/yfcu_Cas9

    Plasmid

    #216423

    Purpose

    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.

    Depositor

    Ellen Bushell

    Article

    Jonsdottir et al bioRxiv 2024.04.20.590404

    Type

    Empty backbone

    Use

    CRISPR; Expression in plasmodium berghei

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSCIP-hTRAC

    Plasmid

    #154094

    Purpose

    Self-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chain

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Insert

    sgRNA and BAIT targeting human TRAC locus
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pdCas12a(RuvC)

    Plasmid

    #186447

    Purpose

    Encodes catalytically inactive Cas12a (D917A, E1006A) from F. novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    dCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D917A, E1006A

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pdCas12b(RuvC)

    Plasmid

    #186450

    Purpose

    Encodes catalytically inactive Cas12b (E848A, D977A) from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    dCas12b
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    E848A, D977A

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCas4_1_2_VA

    Plasmid

    #186438

    Purpose

    Encodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4, Cas1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCas4_1_Δ2_VA

    Plasmid

    #186439

    Purpose

    Encodes Cas4, Cas1, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4, Cas1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCas-mut4_1_2_VA

    Plasmid

    #186441

    Purpose

    Encodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4, Cas1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCas-4/1_2_VB

    Plasmid

    #186442

    Purpose

    Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCas-4/1Δ2_VB

    Plasmid

    #186443

    Purpose

    Encodes Cas4/1, leader, repeat and one spacer from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCas-mut4/1_2_VB

    Plasmid

    #186444

    Purpose

    Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Cas4 domain (K81A)

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCas12(PI)

    Plasmid

    #186448

    Purpose

    Encodes inactive Cas12a containing mutation in the PI domain (K613A, K617A) from F. novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas12a (PI)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    K613A, K617A

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCas12b(PI)

    Plasmid

    #186451

    Purpose

    Encodes inactive Cas12b containing mutation in the PI domain (R122A, G143P) from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas12b (PI)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    R122A, G143P

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCFD3.1-w-dU6:3gRNA

    Plasmid

    #123366

    Purpose

    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).

    Depositor

    Simon Bullock

    Insert

    U6:3-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGMC00024 (aka pCW-Cas9n-Blast)

    Plasmid

    #195315

    Purpose

    Dox inducible lentiviral Cas9 nickase

    Depositor

    Raj Chari

    Insert

    Cas9-nickase
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A nickase

    Promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRARB.1.0-gDNA

    Plasmid

    #132471

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    RARB (RARB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge