We narrowed to 12,503 results for: BASE
-
Plasmid#157839Purposeexpresses split DddAtox-Cas9 construct in mammalian cellsDepositorInsertG1397 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
H446 EF1a RA-ABE6.3 p2A Puro
Plasmid#139104PurposeLentiviral expression of ABE6.3.DepositorInsertABE RA6.3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCpf1-BE0
Plasmid#107678PurposeExpresses dLbCpf1-BE0 in mammalian cellsDepositorInsertdLbCpf1-BE0
UseTagsExpressionMammalianMutationD832A/E925/D1148APromoterCMVAvailable sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330_rActb sgRNA / hSpCas9
Plasmid#172833PurposeMammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_rSyn1 sgRNA / hSpCas9
Plasmid#172840PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLbCpf1-BE0ΔiNLS
Plasmid#107684PurposeExpresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cellsDepositorInsertdLbCpf1-BE0ΔiNLS
UseTagsExpressionMammalianMutationD832A/E925A/D1148APromoterCMVAvailable sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEN_hU6miR-G13-L
Plasmid#25758PurposeEntry vector with human U6 promoter driving mouse G alpha 13 miR30-based shRNA.DepositorInsertG alpha 13 miR-shRNA
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEN_TmiR_G13-L_G12-E
Plasmid#25763PurposeEntry vector with TRE promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.DepositorInsertG alpha 13 and 12 miR-shRNA
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH-CGBE1
Plasmid#198557PurposeExpresses human codon-optimized SpCas9-NRCH-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NRCH-CGBE1-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-CGBE1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gα13-125-NLuc
Plasmid#207155PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA13 tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2DepositorInsertGNA13 (GNA13 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position F(125)DPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gα12-133-NLuc
Plasmid#207156PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNA12 (GNA12 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position A(133)FPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-e1 sgRNA / hSpCas9
Plasmid#172830PurposeMammalian expression of a sgRNA targeting the exon 2 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-e2 sgRNA / hSpCas9
Plasmid#172831PurposeMammalian expression of a sgRNA targeting the exon 2 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_Camk2a sgRNA / hSpCas9
Plasmid#172839PurposeMammalian expression of a sgRNA targeting the intron 17 (last intron) of Camk2a (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 17 of Camk2a under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i5 sgRNA / hSpCas9
Plasmid#172829PurposeMammalian expression of a sgRNA targeting the intron 1 position 5 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i3 sgRNA / hSpCas9
Plasmid#172827PurposeMammalian expression of a sgRNA targeting the intron 1 position 3 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i2 sgRNA / hSpCas9
Plasmid#172826PurposeMammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 P742 miniABEMax V82G
Plasmid#135385PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (P742 )DepositorInsertnSaCas9 P742 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 Q731 miniABEMax V82G
Plasmid#135386PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (Q731 )DepositorInsertnSaCas9 Q731 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 Q737 miniABEMax V82G
Plasmid#135387PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (Q737 )DepositorInsertnSaCas9 Q737 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 S740 miniABEMax V82G
Plasmid#135388PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (S740 )DepositorInsertnSaCas9 S740 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 T128 miniABEMax V82G
Plasmid#135389PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (T128 )DepositorInsertnSaCas9 T128 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 D127 miniABEMax V82G
Plasmid#135366PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (D127 )DepositorInsertnSaCas9 D127 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 D126 miniABEMax V82G
Plasmid#135367PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (D126 )DepositorInsertnSaCas9 D126 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 E739 miniABEMax V82G
Plasmid#135368PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E739 )DepositorInsertnSaCas9 E739 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 E743 miniABEMax V82G
Plasmid#135369PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E743 )DepositorInsertnSaCas9 E743 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 E745 miniABEMax V82G
Plasmid#135370PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E745 )DepositorInsertnSaCas9 E745 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 F733 miniABEMax V82G
Plasmid#135371PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (F733 )DepositorInsertnSaCas9 F733 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 N730 miniABEMax V82G
Plasmid#135372PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N730 )DepositorInsertnSaCas9 N730 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 E125 miniABEMax V82G
Plasmid#135373PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E125 )DepositorInsertnSaCas9 E125 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 E131 miniABEMax V82G
Plasmid#135374PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E131 )DepositorInsertnSaCas9 E131 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 L132 miniABEMax V82G
Plasmid#135375PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (L132 )DepositorInsertnSaCas9 L132 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 N130 miniABEMax V82G
Plasmid#135376PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N130 )DepositorInsertnSaCas9-ABEMax(V82G) N130
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 E735 miniABEMax V82G
Plasmid#135377PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E735 )DepositorInsertnSaCas9 E735 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 G129 miniABEMax V82G
Plasmid#135380PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (G129 )DepositorInsertnSaCas9 G129 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 H119 miniABEMax V82G
Plasmid#135381PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (H119 )DepositorInsertnSaCas9-ABEMax(V82G) H119
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 M732 miniABEMax V82G
Plasmid#135382PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M732 )DepositorInsertnSaCas9 M732 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 M741 miniABEMax V82G
Plasmid#135383PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M741 )DepositorInsertnSaCas9 M741 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
nSaCas9 N120 miniABEMax V82G
Plasmid#135384PurposeExpression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N120 )DepositorInsertnSaCas9 N120 miniABEMax V82G
UseCRISPRTagsExpressionMammalianMutation(V82G)PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
S2 1058 ABEMax
Plasmid#135356PurposeExpression of intradomain insertion of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at position 1058 of nSpyCas9DepositorInsertnSpyCas9-ABEMax aa 1058
UseCRISPRTagsExpressionMammalianMutationWTPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only