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Fucci(CA) hCdt1-iRFP hGeminin-iRFP

Plasmid

#190182

Purpose

Fluorescent reporter vector for visualization of G0 and other cell cycle phases.

Depositor

Toshiro Sato

Article

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

iRFP
hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
iRFP
hGeminin(1/110)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMIH-mRuby2-BAX

Plasmid

#111628

Purpose

Fluorescent fusion protein used to visualise mouse BAX, with hygromycin selection

Depositor

Benjamin Kile

Article

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

Bax (Bax Mouse)

Use

Retroviral

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pMIH-mRuby2-BAK

Plasmid

#111627

Purpose

Fluorescent fusion protein used to visualise mouse BAK, with hygromycin selection

Depositor

Benjamin Kile

Article

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

BAK (Bak1 Mouse)

Use

Retroviral

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCIBN-hTRF1-tagRFP-T

Plasmid

#103811

Purpose

BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-hTRF1-tagRFP-T (CIB1, TERF1 Mustard Weed, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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FUW-hSNCA(D2R)-GFP

Plasmid

#215490

Purpose

Lentiviral expression and easy visualization of mutated alpha-synuclein (2nd amino acid aspartate mutated to arginine) in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

D (second aa) mutated to R

Promoter

Ubc

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-hSNCA(D2E)-GFP

Plasmid

#215491

Purpose

Lentiviral expression and easy visualization of mutated alpha-synuclein (2nd amino acid aspartate mutated to glutamate) in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

D (second aa) mutated to E

Promoter

Ubc

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6-CD63-pHluorin

Plasmid

#130901

Purpose

Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD63-pHluorin (CD63 Aequorea victoria, Human)

Use

Tags

pHluorin

Expression

Mammalian

Mutation

pHluorin inserted between Gln-36 and Leu-37 in ex…

Promoter

CMV

Available since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSUMO3

Plasmid

#52260

Purpose

Bacterial expression of human 6His-tagged SUMO3, SUMO-E1 and SUMO-E2

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-3 (SUMO3 Human)
UBE2l (UBE2I Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Use

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6-CD81-pHluorin

Plasmid

#130903

Purpose

Expression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD81-pHluorin (CD81 Human, aequorea victoria)

Use

Tags

pHluorin

Expression

Mammalian

Mutation

pHluorin inserted between Leu-49 and Gly-50 in ex…

Promoter

CMV

Available since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Sport6-CD9-pHluorin

Plasmid

#130905

Purpose

Expression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD9-pHluorin (CD9 Aequorea victoria, Human)

Use

Tags

pHluorin

Expression

Mammalian

Mutation

phluorin inserted between Asn-50 and Asn-51 in ex…

Promoter

CMV

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Sport6-CD63-pHuji

Plasmid

#130902

Purpose

Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD63-pHuji (CD63 Human, synthetic)

Use

Tags

pHuji

Expression

Mammalian

Mutation

pHuji inserted between Gln-36 and Leu-37 in extra…

Promoter

CMV

Available since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Sport6-CD81-pHuji

Plasmid

#130904

Purpose

Expression of CD81-pHuji for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD81-pHuji (CD81 Human, Synthetic)

Use

Tags

pHuji

Expression

Mammalian

Mutation

pHuji inserted between Leu-49 and Gly-50 in extra…

Promoter

CMV

Available since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSA1

Plasmid

#52261

Purpose

Bacterial expression of human 6His-tagged SUMO1 and SUMO-E1

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-1 (SUMO1 Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Use

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUMO2

Plasmid

#52259

Purpose

Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-2 (SUMO2 Human)
UBE2l (UBE2I Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Use

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Sport6-CD9-pHuji

Plasmid

#130906

Purpose

Expression of CD9-pHuji for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD9-pHuji (CD9 Human, Synthetic)

Use

Tags

pHuji

Expression

Mammalian

Mutation

pHuji inserted between Asn50 and Asn51 in extrace…

Promoter

CMV

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUMO1

Plasmid

#52258

Purpose

Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-1 (SUMO1 Human)
UBE2l (UBE2I Human)
SAE-2 (UBA2 )
SAE-1 (SAE1 Human)

Use

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge