We narrowed to 2,903 results for: BFP

1. pAC-CR7T-gRNA2.1-nlsBFP

   Plasmid

   #170515

   Purpose

   gRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Promoter

   CR7T

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGB3 alpha1 P35S:BFP:T35S (GB3416)

   Plasmid

   #160637

   Purpose

   TU for the constitutive expression of Blue Fluorescent Protein

   Depositor

   Diego Orzaez

   Insert

   BFP
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   35S

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

   Plasmid

   #98975

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 4 and Cas9(D10A)
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONOR-tagBFP-PSM-dTOMATO

   Plasmid

   #100604

   Purpose

   Donor scafold with dTOMATO positive selection module, and tagBFP negative selection module

   Depositor

   Jens Schwamborn

   Article

   Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   dTOMATO-T2A-Puro and tagBFP

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-mTagBFP2-T2A-dre

   Plasmid

   #124832

   Purpose

   Monomeric TagBFP2 and dre recombinase

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-dre
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. AID-mTagBFP2-loxP_myo2_neoR-loxP

   Plasmid

   #194055

   Purpose

   Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   IAA17 (AXR3 Mustard Weed)
   

   Tags

   codon optimized mTagBFP2

   Expression

   Worm

   Promoter

   None

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDRF1 yeBFP2-T2A-mCherry

   Plasmid

   #219851

   Purpose

   equimolar expression of yeBFP2 and mCherry in budding yeast

   Depositor

   Bas Teusink

   Article

   Botman et al bioRxiv 2025.06.27.661975; doi: https://

   Insert

   yeBFP2-T2A-mCherry
   

   Expression

   Yeast

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDRF1-GW yomTagBFP2-T2A-mCherry

   Plasmid

   #205493

   Purpose

   Expression of yomTagBFP2-T2A-mCherry in yeast

   Depositor

   Bas Teusink

   Article

   Botman et al bioRxiv 2025.06.27.661975; doi: https://

   Insert

   yomTagBFP2-T2A-mCherry
   

   Expression

   Yeast

   Available Since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLIX403-EBFP2-ccdB-G418

   Plasmid

   #158556

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EBFP2

   Expression

   Mammalian

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. SOX2-P2A-tagBFP-HDR

    Plasmid

    #163751

    Purpose

    HDR knock-in template for tagging the human endogenous SOX2 gene with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    SOX2 (SOX2 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    P2A-tagBFP

    Mutation

    Does not contain start codon to avoid random inte…

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. dCas9-SunTag(10X)-2A-BFP

    Plasmid

    #227389

    Purpose

    Adhesion module, dCas9-SunTag

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    dCas9-SunTag
    

    Use

    Lentiviral

    Mutation

    Enzymatically dead Cas9

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKLV2-U6gRNA5(Pou5f1-g2)-PGKpuroBFP-W

    Plasmid

    #105036

    Purpose

    Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Pou5f1 (Pou5f1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-mTagBFP2-FRB-Rab7

    Plasmid

    #214273

    Purpose

    Mammalian expression of mTagBFP2-FRB-Rab7 (late endosome marker)

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    mTagBFP2-FRB-Rab7A (RAB7A Canis lupus familiaris (Dog))
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLIX403-EBFP2-ccdB-Blast

    Plasmid

    #158557

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. plenti-arRNA-BFP-backbone

    Plasmid

    #180221

    Purpose

    For the cloning of arRNA-expressing vectors containing BFP fluorescence marker in mammalian cells

    Depositor

    Wensheng Wei

    Article

    Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Str-KDEL_ST-SBP-tagBFP

    Plasmid

    #65266

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-W

    Plasmid

    #105034

    Purpose

    Lentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Ptpn23 (Ptpn23 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2

    Plasmid

    #169531

    Purpose

    Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

    Plasmid

    #67983

    Purpose

    Cas9 activity reporter (control) with GFP and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-U6gRNA5(GFP)-PGKpuro2ABFP-W

    Plasmid

    #200475

    Purpose

    Lentiviral vector expressing gRNA targeting GFP

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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