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Tet-myr-CSK K222R-GFP

Plasmid

#83471

Purpose

Lentiviral vector for doxycycline-inducible expression of membrane targeted kinase dead CSK in mammalian cells

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

CSK (CSK Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

K222R (kinase dead)

Promoter

doxycycline-inducible promoter

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pmCTIP2-N174

Plasmid

#60858

Purpose

Expresses CTIP2 under the EF1a promoter

Depositor

Andrew Yoo

Article

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

CTIP2 isoform b (Bcl11b Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Gata3

Plasmid

#70270

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Gata3

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

GATA binding protein 3 (Gata3 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Ets2

Plasmid

#70272

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Ets2

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Ets2 (Ets2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Tcf4

Plasmid

#139806

Purpose

doxycycline-inducible expression of mouse Tcf4 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Tcf4 (Tcf4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-jYCaMP1

Plasmid

#135423

Purpose

Yellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfection

Depositor

Kaspar Podgorski

Article

Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

Insert

jYCaMP1

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Ascl2

Plasmid

#71151

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Ascl2/Mash2

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Ascl2 (Ascl2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Fos

Plasmid

#131593

Purpose

doxycycline-inducible expression of mouse c-Fos in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

FBJ osteosarcoma oncogene (Fos Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-Klf5

Plasmid

#50787

Purpose

retroviral expression of mouse Klf5

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Klf5 (Klf5 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Cdx2

Plasmid

#128829

Purpose

dox-dependent expression of mouse Cdx2

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Cdx2 (Cdx2 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Bcl11b

Plasmid

#139831

Purpose

doxycycline-inducible expression of mouse Bcl11b in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Bcl11b (Bcl11b Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

Plasmid

#141236

Purpose

AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)

Depositor

Guohong Cui

Article

Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

Insert

NES-jRGECO1a

Use

AAV and Cre/Lox; Cre-off

Tags

6xHIS tag and nuclear export signal

Expression

Mutation

Promoter

human elongation factor-1 alpha (EF-1 alpha)

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-PKHD1-myc

Plasmid

#108407

Purpose

Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.

Depositor

Gregory Germino

Article

Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56

Insert

PKHD1 (PKHD1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-IKZF1

Plasmid

#139807

Purpose

doxycycline-inducible expression of Human IKZF1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Ikzf1 (IKZF1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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LV-hHOXA13

Plasmid

#135561

Purpose

Express hHOXA13 and EGFP in mammalian cells

Depositor

Jose Teixeira

Article

George et al Cell Rep. 2019 Dec 17;29(12):4069-4085.e

Insert

Homeobox A13 (HOXA13 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CBH

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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PB-TET-mKLF4

Plasmid

#20906

Purpose

piggyBac vector with tet inducible mKLF4

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

KLF4 (Klf4 Mouse)

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA5-GFP-PKHD1

Plasmid

#108408

Purpose

Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.

Depositor

Gregory Germino

Article

Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56

Insert

PKHD1 (PKHD1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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ACVR1-TAAS-R206H

Plasmid

#206184

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S190A, S192A, and R206H

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Expression

Mutation

S190A, S192A, R206H

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCCLc-U6-shHMGA2.3-PGK-dTomato

Plasmid

#89606

Purpose

Silencing Hmga2 in human cells

Depositor

Fernando Fierro

Article

Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

Insert

shRNA targeting HMGA2 (HMGA2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCCLc-MNDU3-LIN28A-PGK-EGFP-WPRE

Plasmid

#89607

Purpose

Expression of LIN28A in human cells

Depositor

Fernando Fierro

Article

Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

Insert

LIN28A (LIN28A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

MNDU3

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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