We narrowed to 14,410 results for: nts

1. pcDNA 3.2/V5-DEST NET1A V5 WT

   Plasmid

   #69817

   Purpose

   Expresses NET1A-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   NET1A (NET1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHR-mCherry-p2A-LIC1 Y101A, Y103A, W120A

   Plasmid

   #74606

   Purpose

   expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   full length human dynein light intermediate chain 1 Y101A, Y103A, W120A (DYNC1LI1 Human)
   

   Use

   Lentiviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Y101A, Y103A, W120A

   Promoter

   SFFV

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FUW TetO HA-AMPKa1 AS

   Plasmid

   #69825

   Purpose

   Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   minimal CMV promoter with tetracycline operator

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDNA4TO R99A CoV-2 nsp1 3xFLAG

   Plasmid

   #176061

   Purpose

   Expresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 nsp1 (ORF1ab SARS-CoV-2)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDNA4TO-3XFLAG Halo delta 1-117 nsp1

   Plasmid

   #175423

   Purpose

   Expresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 delta 1-117 nsp1
   

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   nsp1 C-terminus

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA4 3xFLAG Halo delta 122-130 nsp1

   Plasmid

   #175425

   Purpose

   Expresses delta 122-130 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 delta 122-130 nsp1
   

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA4 3xFLAG Halo delta 118-180 nsp1

   Plasmid

   #175424

   Purpose

   Expresses delta 118-180 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 delta 118-180 nsp1
   

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   N-terminus of nsp1

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP-AuroraB L154G/H250Y

   Plasmid

   #108492

   Purpose

   expression of EGFP-AuroraB Analog sensitive (L154G/H250Y)

   Depositor

   Susanne Lens

   Article

   Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

   Insert

   AuroraB (AURKB Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Analog sensitive L154G/H250Y

   Promoter

   CMV

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA4TO-3XFLAG Halo R124A/K125A nsp1

   Plasmid

   #175417

   Purpose

   Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 R124A/K125A nsp1
   

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   R124A and K125A

   Promoter

   CMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCR3-Flag-AuroraB KD (K106R)

    Plasmid

    #108488

    Purpose

    expression of Flag-AuroraB kinase death (K106R)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Kinase death K106A

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJW1759

    Plasmid

    #154315

    Purpose

    NT Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::TEV::AID*::3xFLAG for NT slot
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RCAS-sgATG7

    Plasmid

    #75228

    Purpose

    An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh

    Depositor

    Noor Gammoh

    Article

    Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:

    Insert

    Autophagy Related 7 (Atg7 Mouse)
    

    Use

    Retroviral

    Promoter

    U6

    Available Since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pHR-mCherry-p2A-LIC1 C-terminal half

    Plasmid

    #74603

    Purpose

    expresses LIC1 in mammalian cells; aa 389-523, untagged but cells indicate expression with diffuse mCherry

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    full length human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    amino acids 389-523 of LIC1

    Promoter

    SFFV

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FUW TetO NET1A-V5 WT

    Plasmid

    #69828

    Purpose

    Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)
    

    Use

    Lentiviral

    Tags

    v5

    Expression

    Mammalian

    Promoter

    minimal CMV promoter with tetracycline operator

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pECE M2-SH3PXD2A 8 serines mutated to alanines

    Plasmid

    #69816

    Purpose

    Expresses M2-SH3PXD2A in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    SH3 and PX domain-containing protein 2A (SH3PXD2A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SV40 early promoter

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHR-mCherry-p2A-LIC1 G domain

    Plasmid

    #74602

    Purpose

    expresses LIC1 in mammalian cells; aa 1-389, untagged but cells indicate expression with diffuse mCherry

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    full length human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJW1806

    Plasmid

    #154319

    Purpose

    NT Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    linker::3xMyc for NT slot
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJW1787

    Plasmid

    #154316

    Purpose

    NT Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    linker::TEV::BioTag::AID*::3xFLAG donor for NT slot in the SapTrap cloning system
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJW1348

    Plasmid

    #154311

    Purpose

    NT Donor-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker for NT slot
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pECE M2-SH3PXD2A 6 serines mutated to alanines

    Plasmid

    #69815

    Purpose

    Expresses M2-SH3PXD2A in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    SH3 and PX domain-containing protein 2A (SH3PXD2A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SV40 early promoter

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge