We narrowed to 36,691 results for: ELL
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Plasmid#128386PurposeExpresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruptionDepositorUseLentiviral, Luciferase, and Synthetic BiologyTagsC-terminal luc2 fragment and N-terminal luc2 frag…ExpressionMammalianMutationN terminal carbohydrate recognition domain fused …PromoterEF1A and IRESAvailable SinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only
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pDN-D2irC6kwh
Plasmid#44725DepositorInsertspCMV-D2i promoter
mCherry
UseSynthetic Biology; Expression regulator/reporterTagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg61-PDGFR_EF1a_eGFP
Plasmid#186310PurposePlasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg61-PDGFR
eGFP
TagsHis, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_WT POLH
Plasmid#201671PurposeExpression of WT DNA polymerase eta with an N-terminal HA tag in mammalian cellsDepositorInsertPolymerase eta (POLH Human)
TagsHAExpressionMammalianMutationCoding sequence has been optimised for expression…PromoterCMVAvailable SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLIB-HIS-TEV-CKS1
Plasmid#177013PurposeGenerate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEVDepositorInsertCKS1 (CKS1B Synthetic, Human)
TagsPolyhistidine-TEVExpressionInsectMutationcodon-optimised for insect cell expressionAvailable SinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xAP-1_RE::GrRenilla
Plasmid#124534PurposeAP-1 GreenRenilla luciferase reporterDepositorInsertAP-1GreenRenilla reporter
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA
Plasmid#134924PurposeComponents for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.DepositorInsertCascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
ExpressionMammalianPromoterEF1aAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFP-LacI-NLS
Plasmid#103840PurposeFlourescent marker for lacO arraysDepositorInsertCFP-LacI-NLS
ExpressionMammalianMutationLacI: C19T (silent, L7L), T264C (silent, A88A), C…PromoterSV2Available SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB2-TagRFP-T
Plasmid#103998PurposePlasmid encoding TagRFP-TDepositorInsertTagRFP-T
UseLow copyExpressionBacterialMutationMutations relative to eqFP578 (R32G, K42R, K67R, …PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pdRBFC-NS1-YC
Plasmid#74724PurposeExpress C-terminal YC fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labelingDepositorInsertInfluenza A virus NS1 protein, partial
UseDouble-stranded rna labelingTagsFLAG and YFP C-terminal half domainExpressionPlantPromoterCaMV 35SAvailable SinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTT3 Unc5A-FLAG
Plasmid#72194PurposeExpresses full-length Unc5a with a C-terminal FLAG tagDepositorAvailable SinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Str-STIM1-NN_puromycin
Plasmid#65310PurposeLuminal ER hook only (RUSH system)DepositorInsertSTIM1 mutated for binding motif to microtubule (STIM1 Human)
TagsstreptavidinExpressionMammalianMutation2 amino acids of STIM1 (IP from SxIP motif) were …PromoterCMVAvailable SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTY-EF1a-mKdm2b (del CxxC domain)-Flag
Plasmid#61740PurposeExpress CxxC domain deleted mouse histone demethylase Kdm2bDepositorAvailable SinceFeb. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG5-ERRbeta2
Plasmid#52186PurposeExpresses human ERRbeta2 splice variant in mammalian cellsDepositorInsertEstrogen-related receptor beta (ESRRB Human)
ExpressionMammalianMutationwild type sequence; codon optimized to exclude Ec…PromoterSV40Available SinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSEVA351-Cpf1
Plasmid#192361PurposeCRISPR-based vector for genome editingDepositorInsertCas12a, gRNA scaffold
UseCRISPRAvailable SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe-miR-133
Plasmid#172392PurposeRetroviral expression vector for direct cardiac reprogrammingDepositorInsertmiR-133
UseRetroviralAvailable SinceAug. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag
Plasmid#132402Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059AAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMJ204: pU6-SpuFz1_omegaRNA(350nt)-entry
Plasmid#205268PurposeHuman expression vector for SpuFz1 omegaRNA 5' +275 nt extended scaffold (BbsI guide entry site)DepositorInsertSpuFz1 omegaRNA
ExpressionMammalianAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET-B CFP
Plasmid#108858PurposeEncodes for the fluorescent protein CFPDepositorInsertCFP
ExpressionBacterialAvailable SinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
vHB8
Plasmid#193454PurposeExpression of GFP-Cas9-NLS and gRNA in A. castellaniiDepositorInsertGFP-Cas9-NLS
UseCRISPRTagsGFPPromoterGAPDHAvailable SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only