We narrowed to 15,731 results for: LEA

1. pEGFP-N3-Myr-TYK2

   Plasmid

   #139355

   Purpose

   Expression of membrane-anchored human TYK2 protein fused to GFP

   Depositor

   Sandra Pellegrini

   Article

   Ragimbeau et al J Biol Chem. 2001 Aug 17;276(33):30812-8

   Insert

   TYK2 cDNA with aa 1-16 of human Lck fused to the N-terminus (TYK2 Human)
   

   Tags

   aa 1-16 of human Lck and GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBB0238-TEV-His12

   Plasmid

   #137038

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0238 with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66635.2
   

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTone-gata2aECE-nsGFP

   Plasmid

   #132975

   Purpose

   gata2a endothelial enhancer (x6) and basal promoter driving nuclear-localized sfGFP in pTol1 backbone

   Depositor

   Nathan Lawson

   Article

   Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

   Inserts

   nuclear localized sfGFP
   gata2a endothelial enhancer (x6) with a carp b-actin basal promoter
   

   Use

   Unspecified; Transposon-mediated

   Tags

   6x myc and SV40 NLS

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-NOS-IN133.3xFLAG-WPRE

   Plasmid

   #127869

   Purpose

   pAAV plasmid expressing an NOS-IN133.3xFLAG fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1 (Nos1 Mouse)
   

   Use

   AAV

   Tags

   3xFLAG

   Mutation

   Amino acids 1-133 only

   Promoter

   hSyn

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-30a(+)-C-SH2

   Plasmid

   #111419

   Purpose

   express murine Syk (C)SH2 domain (His 162 to Gln 264), in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p

   Insert

   murine Syk (C)SH2 domain (His 162 to Gln 264), without any tag (Syk Mouse)
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-30a(+)-C-SH2+IA

   Plasmid

   #111418

   Purpose

   express murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p

   Insert

   murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), without any tag (Syk Mouse)
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-30a(+)-N-SH2+IA

   Plasmid

   #111273

   Purpose

   express murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

   Insert

   murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
   

   Tags

   HHHHHHENLYFQG

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDD2034

   Plasmid

   #101377

   Purpose

   Name: pBluescript-Δ611-843REV. Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), 611-843 REV.

   Depositor

   Dirk Dittmer

   Article

   Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

   Insert

   Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), 611-843 REV
   

   Use

   Unspecified

   Mutation

   Blue Heron Biotechnology Inc. bidirectional mutan…

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMGF130

   Plasmid

   #96971

   Purpose

   S. pombe lem2 knockout cassette with hygro resistance gene

   Depositor

   Adam Frost, Wesley Sundquist

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   lem2 (lem2 Fission Yeast)
   

   Expression

   Bacterial

   Mutation

   whole ORF deletion and replaced with hygro resist…

   Promoter

   N/A

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMGF169

    Plasmid

    #96996

    Purpose

    ish1 mCherry insertion cassette with G418 resistance gene

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    ish1 (ish1 Fission Yeast)
    

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    ish1 C-terminal domain fused with mCherry + G418 …

    Promoter

    N/A

    Available Since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgGLDC_2

    Plasmid

    #72887

    Purpose

    CRISPR-Cas9 induced gene disruption

    Depositor

    David Sabatini

    Article

    Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

    Insert

    sgGLDC
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

12. sgGLDC_1

    Plasmid

    #72886

    Purpose

    CRISPR-Cas9 induced gene disruption

    Depositor

    David Sabatini

    Article

    Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

    Insert

    sgGLDC
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA5 FRT TO myc Separase delta 1278-1291

    Plasmid

    #59830

    Purpose

    Allows the integration of myc Separase delta 1278-1291 in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    delta 1278-1291

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. Mouse Nanog locus 3' probe

    Plasmid

    #60037

    Purpose

    Cloned genomic fragment of murine Nanog locus used as probe for Southern Blot analysis

    Depositor

    Jacob Hanna

    Article

    Maza et al Nat Biotechnol. 2015 Jun 22. doi: 10.103

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Unspecified; Cloning

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

15. Hoxd13-3'UTR

    Plasmid

    #31525

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    Hoxd13-3'UTR (Hoxd13 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pCin4-3xFLAG-MKL1 L555A

    Plasmid

    #19851

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 L555A (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Leucine 555 to Alanine

    Available Since

    Feb. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

17. 3691 pGEX4T-3 MLL AD mut.A

    Plasmid

    #8863

    Depositor

    Stanley Korsmeyer

    Article

    Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

    Insert

    MLL min A.D. (KMT2A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    ILPSDIMDFVL --> ILPSDIMDAAA

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

18. 3692 pGEX4T-3 MLL AD mut.B

    Plasmid

    #8864

    Depositor

    Stanley Korsmeyer

    Article

    Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

    Insert

    MLL min A.D. (KMT2A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    ILPSDIMDFVL --> ILPSAAAAFVL

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

19. 3693 pGEX4T-3 MLL AD mut.C

    Plasmid

    #8865

    Depositor

    Stanley Korsmeyer

    Article

    Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

    Insert

    MLL min A.D. (KMT2A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    ILPSDIMDFVL --> ILPSNIMNFVL

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

20. 3694 pGEX4T-3 MLL AD mut.D

    Plasmid

    #8866

    Depositor

    Stanley Korsmeyer

    Article

    Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

    Insert

    MLL min A.D. (KMT2A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    ILPSDIMDFVL --> AAPSDIMDFVL

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only