We narrowed to 12,187 results for: LEA
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Plasmid#213215PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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CRHR2-DuET
Plasmid#213216PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRHR2 (CRHR2 Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry
Plasmid#210504Purposeexpresses NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry component in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry
ExpressionMammalianAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pETSUMO2_Bradyrhizobium-bGSDM
Plasmid#212126PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Bradyrhizobium species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertBradyrhizobium bGSDM
Tags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_CRFmut
Plasmid#208656PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
ExpressionMammalianPromoterCMVAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_CRF0.5
Plasmid#208654PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5
ExpressionMammalianPromoterCMVAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pILL5-NLS-3xmCitrine
Plasmid#192525PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertIAA-leu-resistant-like5 (ILL5 Mustard Weed)
Tags3xmCitrine and NLSExpressionPlantPromoterILL5Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-iCre-pA]-lox-Lyn-EGFP
Plasmid#196875PurposeNeuron-specific expression of LynGFP reporterDepositorInsertLynEGFP
UseCre/LoxExpressionMammalianPromoterQuimeric CAGAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-Nedd1-mOrange2-rCM1
Plasmid#196861PurposeExpression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.DepositorInsertNedd1-mOrange2-rCM1 (Nedd1 Rat)
ExpressionMammalianPromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
AM77_pU6.Sa-gRNA.CLYBL
Plasmid#199237PurposeExpression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locusDepositorInsertS. aureus gRNA spacer
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterRNA polymerase III promoter for human U6 snRNAAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
PlmdCas12e KRAB
Plasmid#188506PurposeExpresses FLAG tagged PlmdCas12e KRABDepositorInsertdCas12e
UseCRISPR and LentiviralTagsNLS-FLAG-KRAB-NLSExpressionMammalianMutationD659A/E756A/D922APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV10-3xFLAG-prs-MCP1
Plasmid#184341PurposeExpress yeast MCP1 in Expi293F cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYeast MCP1 (MCP1 Budding Yeast)
Tags3xFLAG-PreScission cleavage siteExpressionMammalianPromoterCMVAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYJ34-PB-CRISPR-BANCR-E1-gRNA
Plasmid#131077Purposelong non-coding RNA BANCR knock outDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBANCR Exon1 gRNA (BANCR Human)
UseCRISPRExpressionMammalianAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-MCU-tDimer
Plasmid#181865PurposeExpresses tDimer-tagged mitochondrial calcium uniporter (MCU) under control of the CaMKIIa promoterDepositorInsertmitochondrial calcium uniporter (Mcu Mouse)
UseAAV and AdenoviralTagstDimerExpressionMammalianPromoterCaMKIIaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PB1-MontiRed-LOV2
Plasmid#178872PurposeMammalian expression of PB1-MontiRed-LOV2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPB1-MontiRed-LOV2
ExpressionMammalianPromoterCAGAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Venus-79aa-ActA-pEGFP-C1
Plasmid#166763PurposeTo target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.DepositorInsertActA tail anchor (Listeria monocytogenes) (actA Listeria monocytogenes, Synthetic)
TagsVenusExpressionMammalianPromoterCMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
human TLNRD1 pET151
Plasmid#159384PurposeExpresses human TLNRD1 in bacterial cellsDepositorInsertTalin Rod Domain Containing Protein 1 (TLNRD1 Human)
TagsHis-tag, TEV cleavage siteExpressionBacterialPromoterT7Available sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMBP-HRV3C-AcrIF9
Plasmid#141442PurposeExpression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.DepositorInsertAcrIF9
TagsHRV3C cleavage site and Maltose Binding ProteinExpressionBacterialPromoterptacAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A RIGI K270A
Plasmid#167290PurposeLentiviral expression construct encoding a TagBFP in frame with a self-cleaving catalytically dead (K270A) RLR sensor RIG-IDepositorInsertDDX58 (RIGI Human)
UseLentiviralTagstagBFPExpressionMammalianMutationK270APromoterSFFVAvailable sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only