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ET1-human LRRC33

Plasmid

#111603

Purpose

Express human LRRC33 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 (NRROS Human)

Tags

PreScission/TEV/ProteinC/6xHIS/SBP/

Expression

Mammalian

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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miniSOG-Tubulin-6

Plasmid

#57777

Purpose

Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Tubulin (TUBA1B Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCAG eGFP-Oct6

Plasmid

#203790

Purpose

Competitive assay of Oct4 and Oct6 binding to DNA motifs.

Depositor

Thorsten Wohland

Article

Mistri et al EMBO Rep. 2015 Sep;16(9):1177-91. doi: 1

Insert

Pou3f1 (Pou3f1 Mouse)

Tags

mEGFP

Expression

Mammalian

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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mOrange2-Keratin-17

Plasmid

#57965

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Keratin (KRT18 Human)

Tags

mOrange2

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pBS/U6/RNAi SadBs shRNA mouse

Plasmid

#67152

Purpose

pBS/U6 containing shRNA to mouse SADBs

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

BRSK1 (Brsk1 Mouse)

Expression

Mammalian

Promoter

U6

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pAcrF1

Plasmid

#89233

Purpose

Plasmid contains the gene for anti-CRISPR protein AcrF1 (gene 35 from bacteriophage JBD30), with N-terminal 6his tag and TEV protease cleavage site

Depositor

Blake Wiedenheft

Article

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Insert

AcrF1

Tags

6xHis-TEV and FLAG

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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tdminiSOG-H2B-C-10

Plasmid

#57804

Purpose

Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

H2B (H2BC11 Human)

Tags

tdminiSOG

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX SH2A-SH2

Plasmid

#46481

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2A (SH2D4A Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 338-445

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pEMS2117

Plasmid

#49142

Purpose

AAV plasmid with smCBA promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-smCBA-iCre

Use

AAV

Expression

Mammalian

Promoter

smCBA

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX Lnk-SH2

Plasmid

#46451

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Lnk (Sh2b3 Mouse)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 325-466

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-mCherry

Plasmid

#75133

Purpose

Retroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1116

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
mPapaya-Keratin14-N-18

Plasmid

#56346

Purpose

Localization: Cytokeratin, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Keratin14 (KRT14 Human)

Tags

mPapaya

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pGEX Slap2-SH2

Plasmid

#46501

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Slap2 (SLA2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 84-197

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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mOrange2-Vimentin-7

Plasmid

#57977

Purpose

Localization: Intermediate Filaments, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Vimentin (VIM Human)

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
mRuby2-Tubulin-6

Plasmid

#55914

Purpose

Localization: Microtubules, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Tubulin (TUBA1B Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCardinal-MannII-N-10

Plasmid

#56167

Purpose

Localization: Mannosidase/Golgi, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

MannII

Tags

mCardinal

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pGEX p85b(NC)-SH2

Plasmid

#46468

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

p85b(NC) (PIK3R2 Bovine)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 311-717

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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-111 hTF m3

Plasmid

#15450

Depositor

Nigel Mackman

Article

Cui et al J Biol Chem. 1996 Feb 2. 271(5):2731-9.

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

All 3 EGR-1 sites mutated

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only
pGEX Nck2-SH2

Plasmid

#46458

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

NM_001004720.2 (NCK2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 274-380

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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33-G-X1-pEF1-puro

Plasmid

#111604

Purpose

Express human 33-G-X1 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 and GARP chimeria (NRROS Human)

Tags

Flag tag

Expression

Mammalian

Promoter

EF1a

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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