We narrowed to 9,085 results for: mel

1. pEAHISSO1404

   Plasmid

   #97056

   Purpose

   Expressing Sulfolobus solfataricus orf SSO1404 protein

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   S. solfataricus orf SSO1404
   

   Tags

   N-terminal TEV protease cleavable 6xHis tag

   Mutation

   none

   Promoter

   T7

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEAHISPSVORF239

   Plasmid

   #97025

   Purpose

   Expressing Pyrobaculum spherical virus orf239 protein

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   PSV orf 239
   

   Tags

   N-ter TEV protease cleavable 6HIs

   Mutation

   none

   Promoter

   T7

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEAHISPSVORF165A

   Plasmid

   #97024

   Purpose

   Expressing Pyrobaculum spherical virus orf165a protein

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   PSV orf 165a
   

   Tags

   N-ter TEV protease cleavable 6HIs

   Mutation

   none

   Promoter

   T7

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEAHISPSVORF137

   Plasmid

   #97023

   Purpose

   Expressing Pyrobaculum spherical virus orf137 protein

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   PSV orf 137
   

   Tags

   N-ter TEV protease cleavable 6HIs

   Mutation

   none

   Promoter

   T7

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEAHISMRSA1376

   Plasmid

   #97002

   Purpose

   Expressing MRSA sar1376 protein

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   Putative 4-oxalocrotonate tautomerase
   

   Tags

   N-ter TEV protease cleavable 6His

   Mutation

   none

   Promoter

   T7

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEAHISPSVORF131

   Plasmid

   #97022

   Purpose

   Expressing Pyrobaculum spherical virus orf131 protein

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   PSV orf 131
   

   Tags

   N-ter TEV protease cleavable 6HIs

   Mutation

   none

   Promoter

   T7

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEAHISPA4511

   Plasmid

   #97015

   Purpose

   Expressing Pseudomonas aeruginosa orf PA4511 protein

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   P. aeruginosa orf PA4511
   

   Tags

   N-ter TEV protease cleavable 6HIs

   Mutation

   none

   Promoter

   T7

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEAHISMRSATAG

   Plasmid

   #97004

   Purpose

   Expressing MRSA TAG protein

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   DNA-3-methyladenine glycosylase I
   

   Tags

   N-ter TEV protease cleavable 6His

   Promoter

   T7

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-F-Med26-NTD- K74A K75A

   Plasmid

   #49252

   Purpose

   Mammalian expression of flag-tagged human Med26-NTD- K74A K75A

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

   Insert

   Med 26 (MED26 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K74A, K75A, aa 1-339

   Available Since

   Dec. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pDEST-Tol2-PA2-CMV-AB-mCh

    Plasmid

    #160435

    Purpose

    Expresses human Amyloid Beta-mCherry in Zebrafish

    Depositor

    Nicholas Tolwinski

    Article

    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

    Insert

    Human Amyloid Beta peptide (1-42) (APP Human, Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    mCherry

    Promoter

    CMV

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUG34-NES-EGFP-cPHx3

    Plasmid

    #183669

    Purpose

    PtdIns(3,4)P2 biosensor for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    PLEKHA1 (PLEKHA1 Frog, Human)
    

    Tags

    X. laevis map2k1.L(32-44)-EGFP

    Expression

    Yeast

    Mutation

    Amino acids 169-329 (tandem trimer)

    Promoter

    MET25

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1 Sox2 HM a

    Plasmid

    #26353

    Depositor

    Matthew Meyerson

    Article

    Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

    Insert

    Sox2 shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3Basic-ME.2/ApoEpromoter

    Plasmid

    #51436

    Purpose

    This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.2 enhancer

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:

    Insert

    APOE apolipoprotein E (APOE, ME2 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    The ME.2 enhancer is fused upstream of the ApoE g…

    Available Since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-ASNS-OE-IRES-Thy1.1

    Plasmid

    #192947

    Purpose

    Asparagine synthetase overexpression in murine cells

    Depositor

    Ping-Chih Ho

    Article

    Fernandez-Garcia et al Cell Rep. 2022 Nov 15;41(7):111639. doi:

    Insert

    Asparagine synthetase (Asns Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    5'LTR

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-GCaMP5G

    Plasmid

    #31788

    Purpose

    a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

    Depositor

    Douglas Kim, Loren Looger, GENIE Project

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    GCaMP5G
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRE-mClover3-TDP-43ΔNLS

    Plasmid

    #214917

    Purpose

    inducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear puncta

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    TDP-43ΔNLS (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    mClover3

    Mutation

    mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

    Plasmid

    #62723

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV1 and AAV5

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    human synapsin promoter

    Available Since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. TRE-TDP-43ΔNLS-mClover3

    Plasmid

    #214916

    Purpose

    inducible expression of TDP-43 harboring mutant NLS and C-terminal mClover3. Expression yields cytosolic diffuse TDP-43

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    TDP-43ΔNLS (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    mClover3

    Mutation

    mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5

    Plasmid

    #64613

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    Ctnnb1 (Ctnnb1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    S33A, S37A, T41A, S45A

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Syn-FLEX-rc [Chronos-tdTomato]

    Plasmid

    #84481

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    human synapsin promoter

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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