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We narrowed to 18,247 results for: multi

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Showing: 6781 - 6800 of 18247 results

  3. MSP2279

    Plasmid
    #70706
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A and H557A in SaCas9
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70706-plasmid-map-sequence-id-127768

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  4. pU6_(Gluc)_INT(GenPurpClon)

    Plasmid
    #68433
    Purpose
    General Purpose cloning vector for "INT"-like constructs under U6-promoter expression.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    INT construct
    Use
    CRISPR; Cloning vector for generating int-like co…
    Expression
    Mammalian
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68433-plasmid-map-sequence-id-125561

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  6. BAT-lux

    Plasmid
    #20890
    Depositor
    Stefano Piccolo
    Publication
    Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    7xTCF binding sites, Siamois minimal promoter
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    7 multimerized TCF-binding elements fused upstrea…
    Available since
    April 22, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  7. YFP(1-158)-beta-1 in pcDNAI/Amp

    Plasmid
    #54468
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFP (1-158)/beta-1 (GNB1 Human, Aequorea victoria)
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    GBeta-1 was amplified via PCR, which removed an i…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    54468-plasmid-map-sequence-id-112586

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  8. pDN-D2irG4kwh

    Plasmid
    #44723
    Depositor
    Gabor Balazsi
    Publication
    Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    pCMV-D2i promoter
    EGFP
    Use
    Synthetic Biology; Expression regulator/reporter
    Tags
    Rabbit β-globin intron II and WPRE
    Expression
    Mammalian
    Mutation
    Initiator motif (Inr) displaced relative to pCMV-…
    Promoter
    pCMV-D2i
    Available since
    April 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    44723-plasmid-map-sequence-id-61264

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  9. Tom70-LHD(A)-NovoSplit657(A)-mScarlet

    Plasmid
    #255883
    Purpose
    Expression plasmid for NovoSplit657(A), the first part of the NovoSplit system, fused to the mitochondrial localization signal Tom70, mScarlet for fluorescence labeling, and LHD(A)
    Depositor
    Julia Mahamid
    Publication
    Tran et al Science. 2026 Jul 16:eaeb0822. doi: 10.1
    Insert
    NovoSplit657(A)
    Tags
    LHD(A), Tom70, and mScarlet
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    255883-plasmid-map-sequence-id-516336

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  11. cBEST4

    Plasmid
    #234660
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Publication
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3 and tcp830
    Available since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    234660-plasmid-map-sequence-id-472864

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  12. ADORA1-DuET

    Plasmid
    #213173
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ADORA1 (ADORA1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213173-plasmid-map-sequence-id-430741

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  17. p32Lb

    Plasmid
    #158154
    Purpose
    Construct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and FnCpf1, respectively. For plant genome editing.
    Depositor
    Kabin Xie
    Publication
    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    158154-plasmid-map-sequence-id-321492

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Showing: 6781 - 6800 of 18247 results