We narrowed to 7,632 results for: ALP

Showing: 6801 - 6820 of 7632 results

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pCEP4-FLAG-GRM3-VN

Plasmid

#98965

Purpose

FLAG-tagged human mGluR3 fused to N-terminus of split Venus

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

GRM3 (GRM3 Human)

Tags

FLAG (dykddddk) epitope tag, Signal/leader sequen…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET-Sac-Aβ(M1-42/C18C33)

Plasmid

#173759

Purpose

Expresses the AβC18C33 peptide.

Depositor

James Nowick

Article

Zhang et al Biochemistry. 2022 Feb 15;61(4):252-264.

Insert

Aβ(M1-42/C18C33) (APP Human)

Expression

Bacterial

Mutation

changed valine 18 to cysteine; changed glycine 33…

Promoter

T7

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

Plasmid

#164471

Purpose

Expresses sgRNA targeting human GJA1 locus

Depositor

Markus Kuehn

Article

Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

Insert

Gap junction alpha-1 (GJA1) (GJA1 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pD2sE_SC14

Plasmid

#219717

Purpose

DV2 stable E dimer

Depositor

Brian Kuhlman

Article

Kudlacek et al Sci Adv. 2021 Oct 15;7(42):eabg4084. doi

Insert

DV2 sE SC14

Tags

8xHis and human serum albumin (HSA)

Expression

Mammalian

Mutation

A259W, T262R, F279W, T280P

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-NRASQ61K-IRES-Puro

Plasmid

#120570

Purpose

Expresses Flag-HA-6xHIS-N-Ras Q61K fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Q61K

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-TAPBPR-TM

Plasmid

#178645

Purpose

Mammalian expression of FLAG-tagged TAPBPR with MHC TM

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

TAPBPR (TAPBPL Human)

Tags

FLAG and Signal/leader sequence from HLA class I …

Expression

Mammalian

Mutation

switched transmembrane domain with that of MHC-I

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pRRLHygro-pEF1a-p53ash-mKate2-splitmVenusC

Plasmid

#69583

Purpose

Expresses p53 tagged with mKate2 and split C-term mVenus

Depositor

Galit Lahav

Article

Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

Insert

p53 (TP53 Human)

Use

Lentiviral

Tags

mKate2 and split mVenus - C term

Expression

Mammalian

Mutation

7 silent mutations in p53 DNA to prevent targetin…

Promoter

EF1alpha

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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hIL23p19 pEF6-V5 (MJS_004)

Plasmid

#72492

Purpose

Expression of human IL23p19 in mammalian cells (V5 tag)

Depositor

Matthew Sweet

Article

Ramnath et al Immunol Cell Biol. 2015 Oct;93(9):771-9.

Insert

IL23p19 (IL23A Human)

Tags

His/V5

Expression

Mammalian

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 hSCN5A_deltaSIV

Plasmid

#145771

Purpose

expresses human Nav1.5 lacking the SIV motif in mammalian cells

Depositor

Hugues Abriel

Article

Jespersen et al Biochem Biophys Res Commun. 2006 Oct 6;3

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Tags

none

Expression

Mammalian

Mutation

Stop codon at Serine 2013

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pFIN-RK-GFP-WPRE

Plasmid

#44358

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

RK-GFP (MAPK14 Synthetic, Human)

Use

Lentiviral

Promoter

human rhodopsin kinase (pos 2557-2853)

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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PZac2.1 gfaABC1D-Cx43-GFP

Plasmid

#176860

Purpose

Expresses Cx43 fused with GFP at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Cx43-eGFP (Gja1 Mouse)

Use

AAV

Tags

GFP

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-Gnaq

Plasmid

#170298

Purpose

Encoding mouse Gnaq

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A recombinant mouse Gnaq (Gnaq Mouse)

Tags

N/A

Expression

Mammalian

Mutation

Asilent mutation was introduced into the sgRNA-ta…

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLL5.0-Anillin shRNA

Plasmid

#187270

Purpose

Lentiviral expression of shRNA targeting Anillin

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

shRNA for Anillin (ANLN Human)

Use

Lentiviral

Tags

EGFP

Promoter

U6

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-NRAS-IRES-Puro

Plasmid

#120569

Purpose

Expresses Flag-HA-6xHIS-N-Ras wildtype fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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SARS-COV2-NSP1-HA-BASU

Plasmid

#154071

Purpose

Express SARS-CoV-2 NSP1 gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-Puma-pEGFP-C1

Plasmid

#166739

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, Puma

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Puma (BBC3 Human)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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aL-EGFP

Plasmid

#103879

Purpose

full-length human aL with EGFP at betapropeller

Depositor

Timothy Springer

Article

Nordenfelt et al Nat Commun. 2017 Dec 11;8(1):2047. doi:

Insert

full-length human aL with EGFP in betapropeller (ITGAL Human)

Tags

tension sensor module

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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SOX17-NLS-tdTomato-EPG

Plasmid

#210466

Purpose

Donor plasmid for knock-in NLS-tdTomato into the human SOX17 locus

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived puromycin-EGFP selection cassette (SOX17 Human)

Use

CRISPR

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLV Cbx5-V5

Plasmid

#175105

Purpose

Lentiviral expression of mouse Cbx5-V5

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Cbx5 (Cbx5 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-sTagRFP-TUBA1B

Plasmid

#207769

Purpose

Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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