We narrowed to 11,713 results for: NSI

1. pSIL-eGFP-shACTN1

   Plasmid

   #52676

   Purpose

   shRNA against α-actinin-1 with eGFP transfection marker

   Depositor

   Johannes Hell

   Article

   Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

   Insert

   ACTN1 (Actn1 Rat)
   

   Use

   RNAi

   Tags

   CMV-EGFP

   Promoter

   U6

   Available Since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti 4.1 Ex miR200c-141

   Plasmid

   #35534

   Purpose

   3rd generation lentiviral vector

   Depositor

   Greg Goodall

   Article

   Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

   Insert

   miR200c-141 genomic cluster (MIR200C, MIR141 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmGFP10C-­Tau

   Plasmid

   #71433

   Purpose

   Split GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71434

   Depositor

   Henri Huttunen

   Article

   Brunello et al Sci Rep. 2016 Jul 27;6:30498. doi: 10.10

   Insert

   Tau (0N4R) (MAPT Human)
   

   Tags

   GFP (1-212)

   Expression

   Mammalian

   Available Since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pNCST-AvicFP3

   Plasmid

   #129506

   Purpose

   Expresses AvicFP3 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AvicFP3
   

   Expression

   Bacterial

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.Syn.NES-jRCaMP1b.WPRE.SV40

   Plasmid

   #100851

   Purpose

   AAV expression of jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1 and AAV9

   Insert

   jRCaMP1b
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

   Plasmid

   #167296

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   BAX (BAX Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MAC_C_CH60

   Plasmid

   #111662

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   CH60 (HSPD1 Human)
   

   Expression

   Mammalian

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETduet+sfGFP-TetR-p300HAT

   Plasmid

   #157795

   Purpose

   Expression of sfGFP-TetR-p300HAT fusion protein

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   sfGFP-TetR-p300 (EP300 Human)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFP5380 LAMP1-SBP- mCherry-FIREtag

   Plasmid

   #216725

   Purpose

   Expresses LAMP1-mCherry-FIREtag in mamallian cells

   Depositor

   Franck Perez

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   LAMP-1 (LAMP1 Human)
   

   Tags

   FIREtag, Streptavidin Binding Protein (SBP), and …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEM-Cas9HF1

    Plasmid

    #89961

    Purpose

    Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    Cas9HF1
    

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. CLYBL-TO-PAX7

    Plasmid

    #204346

    Purpose

    Knock-in vector to insert a dox-inducible PAX7 transgene into the CYBL locus in human pluripotent stem cells. PAX7 induction results in myogenic differentiation.

    Depositor

    Ivo Lieberam

    Article

    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

    Insert

    human PAX7
    

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST40-2XHA-Src-Y530F

    Plasmid

    #140318

    Purpose

    Expresses HA-tagged human Src-Y530F in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    Y530F

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-Cepheid1s-ST-WPRE

    Plasmid

    #214968

    Purpose

    Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for better photostability

    Depositor

    Peng Zou

    Article

    Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

    Insert

    Cepheid1s-ST
    

    Use

    AAV

    Promoter

    human Synapsin

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)

    Plasmid

    #158953

    Purpose

    Express human RAD51(T309A) mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

    Insert

    RAD51 (RAD51 Human)
    

    Tags

    2X FLAG 2X STREP

    Expression

    Mammalian

    Mutation

    changed threonine 309 to alanine

    Promoter

    CMV

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. rAAV-U6-MeCP2shRNA-CamKIIa-mCherry

    Plasmid

    #169704

    Purpose

    MeCP2 Knockdown

    Depositor

    Ana Oliveira

    Article

    Gulmez Karaca et al Neurobiol Learn Mem. 2018 Mar;149:84-97.

    Insert

    shRNA against MeCp2 (Mecp2 Mouse)
    

    Use

    AAV

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK5-HA-GST-4EBP1-Low GC

    Plasmid

    #69018

    Purpose

    expresses tagged 4EBP1

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    EIF4EBP1 (EIF4EBP1 Human)
    

    Tags

    GST and HA

    Expression

    Mammalian

    Mutation

    lowGC content, wt is ~70% GC rich. Still, it code…

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCdOpt-BMX

    Plasmid

    #203929

    Purpose

    This plasmid contains a codon optimized BleMX drug resistance cassette for generating gene deletions in C. glabrata, C. auris, C. albicans or S. cerevisiae

    Depositor

    Scott Briggs

    Article

    Gregor et al mSphere. 2023 Nov 6:e0031123. doi: 10.11

    Insert

    BleMX
    

    Expression

    Yeast

    Mutation

    BleMX was codon optimized for Candida albicans or…

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST40-2XFL-Src-K298M

    Plasmid

    #140315

    Purpose

    Expresses Flag-tagged human Src-K298M in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    K298M

    Promoter

    CMV

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. FGFR1-optoDroplet

    Plasmid

    #111509

    Purpose

    For optogenetic clustering of FGFR1

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

    Insert

    FGFR1 (FGFR1 Human)
    

    Use

    Lentiviral

    Tags

    Cry2, FUS, Fusion Red, and Myristoylation sequenc…

    Promoter

    SFFV

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-anti-VEGF(Nb35) PAGER(TF)

    Plasmid

    #230000

    Purpose

    Expresses anti-VEGF PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    IL2SP-Aro6-Nb35-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    V360A/R361A on KORD

    Promoter

    CMV

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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