We narrowed to 11,809 results for: emb
-
Plasmid#169487PurposeTier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) for membrane localization of fusion proteins attached to a secretion signal Igk (PhCMV-IgkSS-TM-pA).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPCMV-driven membrane localization
ExpressionMammalianPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1(K38A)-StrepII
Plasmid#174423PurposeExpresses human Drp1 Isoform 3 with K38A mutation in bacteriaDepositorInsertDrp1 (DNM1L Human)
Tags6xHis(TEV) and StrepIIExpressionBacterialMutationK38APromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA
Plasmid#170999PurposeHiLITR protease with MAVS [R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[R537A]
UseLentiviral and Synthetic BiologyExpressionMammalianMutationMAVS(tmd)[R537A]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA4
Plasmid#171002PurposeHiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]
UseLentiviral and Synthetic BiologyExpressionMammalianMutationMAVS(tmd)[Y536delinsFIVLI]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgMTOR_49
Plasmid#125132PurposeTargeting 49th exon of human MLST8 gene by CRISPR-Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMTOR (MTOR Human)
UseCRISPRExpressionMammalianAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_dHMGA_pET28
Plasmid#167329PurposeExpresses TFAM dHMGA mutant in bacterial cellsDepositorInsertTFAM (TFAM Human)
Tags6XHisExpressionBacterialMutationTruncation mutant containing linker, HMGB, and C-…PromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pscPP7(2xWT)-EGFP
Plasmid#140261PurposePP7CP-dependent translational repressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTwo PP7CP-binding sites embedded in scaffold
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_tacI
Plasmid#155170PurposeBaSP and CuCBP co-expression: tacI promoter controls BaSP expressionDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_2_araBAD
Plasmid#155176Purposesee pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBICI_2_strong
Plasmid#155168Purposesee pBICI_strong: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Oct4deltaPE-53BP1mCherry
Plasmid#141122PurposeConstruct used to generate a transgenic mouse strain with expression of 53BP1-mCherry in embryonic germ cellsDepositorAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
TB:2xP53_RE:MiniP
Plasmid#133881Purpose2 copies of the p53 DNA binding motifDepositorInsert2 copies of the p53 DNA binding motif
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Blunt-U6-HES7-STOP-gRNA
Plasmid#130933PurposegRNA for tag human HES7DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHES7-STOP-gRNA (HES7 Human)
ExpressionMammalianPromoterU6Available sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL942
Plasmid#119721Purposeprecursor slow-folding rbsB with the mutations Alanine 248 Threonine, Alanine 188 Cysteine and Glutamic acid 246 CysteineDepositorInsertprecusor slow-folding ribose import binding protein, rbsB, mutations A248T, A118C and E246C
TagsnoneExpressionBacterialMutationAlanine 248 to Threonine, Alanine 188 to Cysteine…PromoterT7Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBV163
Plasmid#108364PurposeUsed to replace C. glabrata YAP1 promoter with the stronger TDH3 promoter.DepositorInsertKnock-in Cg TDH3 promoter in Cg YAP1 gene
UseReplace c.glabrata yap1 promoter with c. glabrata…PromoterTDH3Available sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-G2ZYP2
Plasmid#103110PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertG2ZYP2(Cas9 coding gene from Ralstonia syzygii R24)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-B8I085
Plasmid#103089PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertB8I085(Cas9 coding gene from Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pPD3C
Plasmid#86971PurposeExpresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coliDepositorInsertPCNA3 fused to phosphite dehydrogenase and P450cam
TagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, N106R in PCNA3, silent mutat…PromoterT7 promoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human NCKX4-AS-short (pcDNA3.1+)
Plasmid#75210PurposeMammalian expression of SLC24A4DepositorInsertSLC24A4 (SLC24A4 Human)
ExpressionMammalianMutationalternatively spliced exon absentPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only