We narrowed to 10,337 results for: plasmids 101

1. pAAV-FLEX-SaCas9-U6-sgSlc17a6

   Plasmid

   #124847

   Purpose

   Mutagenesis of Slc17a6 with SauCas9

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a6 gRNA (Slc17a6 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. LV-Sox10MCS5-GFP

   Plasmid

   #115783

   Purpose

   This plasmid encodes for Sox10-MCS5-GFP reporter. Cell carrying this construct expresses green fluorescence under the control of SOX-MCS5 enhancer conjugated with cfos basal promoter.

   Depositor

   Fraser Sim

   Article

   Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1

   Insert

   mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
   

   Use

   Lentiviral

   Tags

   cfos basal promoter conjugated MCS5 promoter fuse…

   Expression

   Mutation

   Promoter

   SOX10 Multiple Species Conserved enhancer element…

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-HDR-mEGFP-Actin

   Plasmid

   #119870

   Purpose

   AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse Beta Actin

   Depositor

   Ryohei Yasuda

   Article

   Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

   Insert

   beta Actin (Actb Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiPGK Puro DEST PKAKTRClover

   Plasmid

   #59153

   Purpose

   Lentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   PKA (PRKACA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Sept. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH

   Plasmid

   #203840

   Purpose

   Kir2.1 expression for silencing neurons and mScarlet-KASH expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   Kir2.1 (Kcnj2 Mouse)
   

   Use

   AAV

   Tags

   2A-mScarlet-KASH

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAS_4xMma PylT_FLAG-PcKRS

   Plasmid

   #140022

   Purpose

   Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   PcKRS (pylS Methanosarcina bakeri)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   M241F, A267S, Y271C, L274M

   Promoter

   EF1

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAND1-T2A-dTom-PGK-Neo

   Plasmid

   #209838

   Purpose

   Tomato reporter plasmid targeting the human HAND1 gene

   Depositor

   Matthew Birket

   Article

   Lynch et al Stem Cell Res. 2024 Mar 4;77:103342. doi

   Insert

   HAND1-T2A-dTomato-PGK-Neo-HAND1 (HAND1 Human)
   

   Use

   Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEX-SaCas9-U6-sgGrin1

   Plasmid

   #124852

   Purpose

   Mutagenesis of Grin1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin1 (Grin1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS_4xMma PylT_FLAG-AcKRS

   Plasmid

   #140021

   Purpose

   Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   AcKRS-3 (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   L301M, L305I, Y306F, L309A, C348F

   Promoter

   EF1

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-HDR-mEGFP-camk2a

    Plasmid

    #104589

    Purpose

    AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse camk2a

    Depositor

    Ryohei Yasuda

    Article

    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

    Insert

    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    None

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS_4xG1 PylT FLAG-G1 PylRS Y125A

    Plasmid

    #154769

    Purpose

    plasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y125A

    Promoter

    EF1

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF1a_ETV2_P2A_Hygro_Barcode

    Plasmid

    #120436

    Purpose

    Barcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    ETV2 (ETV2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-FLEX-SaCas9-U6-sgGria2

    Plasmid

    #124854

    Purpose

    Mutagenesis of Gria2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria2 (Gria2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. B52 + SMARCAL1 sgSTOP

    Plasmid

    #100715

    Purpose

    B52 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting SMARCAL1 (cloned using BbsI)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-FLEXfrt-SaCas9-U6-sgGria2

    Plasmid

    #124868

    Purpose

    Mutagenesis of Gria2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria2 (Gria2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-mir144~451 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164793

    Purpose

    Express miR-144~451 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-144~451 (MIR451A, MIR144 Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-SEMPER_ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry

    Plasmid

    #221081

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and msfBFO[r5M] and emiRFP670 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-FLEXfrt-SaCas9-U6-sgGrin1

    Plasmid

    #124865

    Purpose

    Mutagenesis of Grin1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin1 (Grin1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. hSyn-somAion-citrine

    Plasmid

    #192570

    Purpose

    AAV-vector for optogenetic long-term silencing of neurons

    Depositor

    Simon Wiegert

    Article

    Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

    Insert

    somAion
    

    Use

    AAV

    Tags

    Citrine - soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    human synapsin

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti HsATP10B - EmGFP

    Plasmid

    #204474

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP10B WT fused with EmGFP

    Depositor

    Veerle Baekelandt

    Article

    Wouters et al Biochim Biophys Acta Mol Cell Res. 2024

    Inserts

    ATP10B (ATP10B Human)
    EmGFP
    

    Use

    Lentiviral

    Tags

    EmGFP

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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