We narrowed to 11,283 results for: Vars;
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Plasmid#240406Purposeexpresses N-His tagged NocO H279A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmidDepositorInsertnocO
TagsHisExpressionBacterialMutationM1 of native sequence not included in insert, His…PromoterT7Available SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-solo-NHis6-NocO-E94A
Plasmid#240401Purposeexpresses N-His tagged NocO E94A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmidDepositorInsertnocO
TagsHisExpressionBacterialMutationM1 of native sequence not included in insert, Glu…PromoterT7Available SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-Illusia(Y783F)
Plasmid#222595PurposeGateway entry vector with a phospho-mutant (Y783F) variant of the Illusia FRET reporter for integrin phosphorylationDepositorInsertIllusia(Y783F)
UseGateway entry vectorMutationMutation in the NPxY(783) site equivolent to Y783…PromoternoneAvailable SinceMay 29, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPB.DEST-Illusia(Y783F)
Plasmid#222596PurposePiggybac expression vector with a phospho-mutant (Y783F) variant of the Illusia FRET reporter for integrin phosphorylationDepositorInsertIllusia(Y783F)
UseTransposon-based stable expressionExpressionMammalianMutationMutation in the NPxY(783) site equivolent to Y783…PromoterCAGAvailable SinceMay 29, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENTR221-Illusia(Y795F)
Plasmid#222597PurposeGateway entry vector with a phospho-mutant (Y795F) variant of the Illusia FRET reporter for integrin phosphorylationDepositorInsertIllusia(Y795F)
UseGateway entry vectorMutationMutation in the NPxY(795) site equivolent to Y795…PromoternoneAvailable SinceMay 29, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPB.DEST-Illusia(Y795F)
Plasmid#222598PurposePiggybac expression vector with a phospho-mutant (Y795F) variant of the Illusia FRET reporter for integrin phosphorylationDepositorInsertIllusia(Y795F)
UseTransposon-based stable expressionExpressionMammalianMutationMutation in the NPxY(795) site equivolent to Y795…PromoterCAGAvailable SinceMay 29, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
TMEM16A-I547K-peGFP-N1
Plasmid#228583PurposeExpresses mouse TMEM16A-I547K-eGFP in mammalian cellsDepositorInsertTMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)
ExpressionMammalianMutationI547KAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
TMEM16A-E551K-peGFP-N1
Plasmid#228584PurposeExpresses mouse TMEM16A-E551K-eGFP in mammalian cellsDepositorInsertTMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)
ExpressionMammalianMutationE551KAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-flex-iGABASnFR2n-WPRE
Plasmid#218881PurposeAAV-mediated expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseAAV and Cre/LoxExpressionMammalianMutationS99A F104H R168PPromoterCAGAvailable SinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE
Plasmid#218873PurposeAAV-mediated expression of improved GABA sensor control (no change in fluorescence)DepositorHas ServiceAAV1 and AAV5InsertiGABASnFR2(no bind)
UseAAVExpressionMammalianMutationS99A F102G R168PPromoterGFAPAvailable SinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-NES-jRGECO1a-WPRE
Plasmid#216276PurposeAAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of red fluorescent calcium indicator jRGECO1aDepositorInsertNES-jRGECO1a
UseAAV and Cre/LoxTags6XHIS and XpressPromoterhuman synapsin 1 (hSyn)Available SinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-iGABASnFR2(no bind)-WPRE
Plasmid#218876PurposeAAV-mediated expression of improved GABA sensor control (no change in fluorescence)DepositorHas ServiceAAV1 and AAV5InsertiGABASnFR2(no bind)
UseAAVExpressionMammalianMutationS99A F102G R168PPromoterSynapsinAvailable SinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnTS-E1015A
Plasmid#213415PurposeVinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).DepositorInsertVcnTS-E1015A (VCL Chicken, Synthetic)
ExpressionMammalianMutationMutated vinculin glutamic acid 1015 to alanine (E…PromoterCMVAvailable SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnTS-E1021A
Plasmid#213416PurposeVinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).DepositorInsertVcnTS-E1021A (VCL Chicken, Synthetic)
ExpressionMammalianMutationMutated vinculin glutamic acid 1021 to alanine (E…PromoterCMVAvailable SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnCS-E1015A
Plasmid#213412PurposeVinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).DepositorInsertVcnCS-E1015A (VCL Chicken, Synthetic)
ExpressionMammalianMutationMutated vinculin glutamic acid 1015 to alanine (E…PromoterCMVAvailable SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnCS-E1021A
Plasmid#213413PurposeVinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).DepositorInsertVcnCS-E1021A (VCL Chicken, Synthetic)
ExpressionMammalianMutationMutated vinculin glutamic acid 1021 to alanine (E…PromoterCMVAvailable SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_EF1a_DIO_HA/FLAG_muMASS_LF
Plasmid#213394PurposeLoss of function variant of uMASS_1 opioid sensor. AAV virus production for Cre dependent expression.DepositorInsertuMASS_LF
UseAAV and Cre/LoxExpressionMammalianAvailable SinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
GL4.10 E2E1- Prg4 promoter-luciferase
Plasmid#195035PurposePrg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vectorDepositorInsertPrg4 promoter plus Enhancer 1 and Enhancer 2 (PRG4 Bovine)
UseLuciferaseAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10 E3E2E1-Prg4 promoter-luciferase
Plasmid#195036PurposePrg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vectorDepositorInsertPrg4 promoter plus Enhancer 1, Enhancer 2, and Enhancer 3 (PRG4 Bovine)
UseLuciferaseAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10 E4E3E2E1-Prg4 promoter-luciferase
Plasmid#195037PurposePrg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vectorDepositorInsertpGL4.10 E4E3E2E1-Prg4 promoter-luciferase (PRG4 Bovine)
UseLuciferaseAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
186_pETcon_SARS2_XBB1-5
Plasmid#208086Purposeyeast surface display of the SARS-CoV-2 XBB.1.5 variant RBDDepositorInsertSARS-CoV-2 XBB.1.5 RBD (S Budding Yeast, SARS-CoV-2 virus)
TagsHA and c-MycExpressionYeastAvailable SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHelz_AH
Plasmid#148881PurposeInsect Expression of DmHelzDepositorInsertDmHelz (CG9425 Fly)
ExpressionInsectMutationtwo silent an two non silent G1374S, R2076G mutat…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHelz_AH
Plasmid#148885PurposeInsect Expression of DmHelzDepositorInsertDmHelz (CG9425 Fly)
ExpressionInsectMutationtwo silent an two non silent G1374S, R2076G mutat…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHelz_1213-2103_AH
Plasmid#148887PurposeInsect Expression of DmHelz_1213-2103DepositorInsertDmHelz_1213-2103 (CG9425 Fly)
ExpressionInsectMutationtwo silent an two non silent G1374S, R2076G mutat…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHelz-E958Q_AH
Plasmid#148888PurposeInsect Expression of DmHelzE958QDepositorInsertDmHelzE958Q (CG9425 Fly)
ExpressionInsectMutationtwo silent an two non silent G1374S, R2076G mutat…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4E-T-YLFWtoAADD_AB
Plasmid#148373PurposeInsect Expression of Dm4E-T-YLFWtoAADDDepositorInsertDm4E-T-YLFWtoAADD (4E-T Fly)
ExpressionInsectMutationtwo non silent mutations H611R and N659S and 8 si…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-DmXRN1-V5His6_J
Plasmid#146708PurposeInsect Expression of DmXRN1DepositorInsertDmXRN1 (pcm Fly)
ExpressionInsectMutationone silent mtuation compared to the sequence give…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-F2V2-siRNAres_F
Plasmid#146330PurposeInsect Expression of DmAGO1-F2V2DepositorInsertDmAGO1-F2V2 (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations compared to…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-F629V-siRNAres_F
Plasmid#146332PurposeInsect Expression of DmAGO1-F629VDepositorInsertDmAGO1-F629V (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations compared to…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-AvrII-dsRNAres_L
Plasmid#146826PurposeInsect Expression of DmGW182-dsRNAresDepositorInsertDmGW182-dsRNAres (gw Fly)
ExpressionInsectMutationTwo non silent mutations V704A and N799S and one …Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only