12,469 results

1. pLKO.1-Tet-Puro-shMMERVK9c_2 (inducible)

   Plasmid

   #189958

   Purpose

   shRNA mediated knockdown

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   ERV_MMERVK9c
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGFP-nE-mHP1alpha

   Plasmid

   #181895

   Purpose

   Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   nE-mHP1alpha (Cbx5 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   S11E, S12E, S13E, S14E

   Promoter

   CMV

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mTln1-F2F3-P229L-pET151

   Plasmid

   #177879

   Purpose

   Expresses mouse Talin 1 F2F3-P229L domains in bacterial cells

   Depositor

   Ben Goult

   Article

   Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

   Insert

   mouse Talin 1 F2F3 P229L mutant (Tln1 Mouse)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   changed Proline 229 to Leucine (P229L)

   Promoter

   T7

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mTln1-F2-pET151

   Plasmid

   #177870

   Purpose

   Expresses mouse Talin 1 F2 domain in bacterial cells

   Depositor

   Ben Goult

   Article

   Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

   Insert

   mouse Talin 1 F2 domain (Tln1 Mouse)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mTln1-F2-P229S-pET151

   Plasmid

   #177871

   Purpose

   Expresses mouse Talin 1 F2-P229S domain in bacterial cells

   Depositor

   Ben Goult

   Article

   Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

   Insert

   mouse Talin 1 F2 P229S mutant (Tln1 Mouse)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   changed Proline 229 to Serine (P229S)

   Promoter

   T7

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mTln1-F2-P229L-pET151

   Plasmid

   #177876

   Purpose

   Expresses mouse Talin 1 F2-P229L domain in bacterial cells

   Depositor

   Ben Goult

   Article

   Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

   Insert

   mouse Talin 1 F2 P229L mutant (Tln1 Mouse)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   changed Proline 229 to Leucine (P229L)

   Promoter

   T7

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mTln1-F2F3-pET151

   Plasmid

   #177877

   Purpose

   Expresses mouse Talin 1 F2F3 domains in bacterial cells

   Depositor

   Ben Goult

   Article

   Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

   Insert

   mouse Talin 1 F2F3 domain (Tln1 Mouse)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mTln1-F2F3-P229S-pET151

   Plasmid

   #177878

   Purpose

   Expresses mouse Talin 1 F2F3-P229S domains in bacterial cells

   Depositor

   Ben Goult

   Article

   Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

   Insert

   mouse Talin 1 F2F3 P229S mutant (Tln1 Mouse)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   changed Proline 229 to Serine (P229S)

   Promoter

   T7

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDR111_mannose_Stat1Klf6

   Plasmid

   #188400

   Purpose

   Expresses mouse transcription factors Stat-1 and Klf6 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.

   Depositor

   Christopher Contag

   Article

   Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

   Inserts

   Stat-1 (Stat1 Escherichia coli, Mouse)
   Klf6 (Klf6 Mouse)
   

   Use

   Tags

   PhoD secretion peptide

   Expression

   Bacterial

   Mutation

   Promoter

   D-mannose

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDR111_mannose_Klf4Gata-3

    Plasmid

    #188401

    Purpose

    Expresses mouse transcription factors Klf4 and Gata-3 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Inserts

    Klf4 (Klf4 Mouse)
    Gata-3 (Gata3 Mouse)
    

    Use

    Tags

    PhoD secretion peptide

    Expression

    Bacterial

    Mutation

    See Depositor Comments Below

    Promoter

    D-mannose

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pv6_CBX1_2xChromo-W42A

    Plasmid

    #179394

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo(W42A)

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX1_2xChromo(W42A)
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Chromodomain W42A

    Promoter

    CAGGS

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pv6_CBX7_Chromo_TAF3_Phd-DW890/891AA

    Plasmid

    #179402

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd(DW890/891AA)

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7_Chromo - TAF3_Phd(DW890/891AA)
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Phd domain DW890/891AA

    Promoter

    CAGGS

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pv7_MBD1_MBD_noEGFP

    Plasmid

    #179406

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MBD1_MBD
    

    Use

    Tags

    AviTag

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pv6_MECP2_MBD-T158M

    Plasmid

    #179410

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(T158M)

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MECP2_MBD(T158M) (Mecp2 Mouse)
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    MBD T158M

    Promoter

    CAGGS

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pv6_CBX7_Chromo-W35A_TAF3_Phd

    Plasmid

    #179401

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo(W35A) - TAF3_Phd

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7_Chromo(W35A) - TAF3_Phd
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Chromodomain W35A

    Promoter

    CAGGS

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pv7_CBX1_2xChromo_noEGFP

    Plasmid

    #179395

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo_noEGFP

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX1_2xChromo_noEGFP
    

    Use

    Tags

    AviTag

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pv6_MBD1_MBD-R22A

    Plasmid

    #179405

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD(R22A)

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MBD1_MBD(R22A)
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    MBD R22A

    Promoter

    CAGGS

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. px459-H3.3 (H3f3b) sgRNA

    Plasmid

    #186931

    Purpose

    H3f3b sgRNA used for genomic targeting at H3f3b C-terminal

    Depositor

    Zhiguo Zhang

    Article

    Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

    Insert

    H3f3b sgRNA (H3f3b Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. px459-H3.1 (Hist1h3g) sgRNA

    Plasmid

    #186930

    Purpose

    Hist1h3g sgRNA used for genomic targeting at Hist1h3g C-terminal

    Depositor

    Zhiguo Zhang

    Article

    Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

    Insert

    Hist1h3g sgRNA (H3c8 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Anti-Kv2.1 K+ channel [D4/40R]

    Plasmid

    #182096

    Purpose

    Mammalian Expression Plasmid of anti-Kv2.1 K+ channel (Rat). Derived from hybridoma D4/40.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Kv2.1 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnb1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Anti-Pan-Gamma-protocadherin-A [N144/32R]

    Plasmid

    #182105

    Purpose

    Mammalian Expression Plasmid of anti-Pan-Gamma-protocadherin-A. Derived from hybridoma N144/32.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Pan-Gamma-protocadherin-A () recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Anti-SAPAP1/2 [N238/31R]

    Plasmid

    #182110

    Purpose

    Mammalian Expression Plasmid of anti-SAPAP1/2 (). Derived from hybridoma N238/31.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-SAPAP1/2 () recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3

    Plasmid

    #73542

    Purpose

    Expression of sgRNA targeting LINE-1 and TagBFP

    Depositor

    Constance Ciaudo

    Article

    Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

    Insert

    LINE-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1

    Plasmid

    #73543

    Purpose

    Expression of sgRNA targeting LINE-1 and TagBFP

    Depositor

    Constance Ciaudo

    Article

    Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

    Insert

    LINE-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. psiCHECK2-mMOV10-3'UTR

    Plasmid

    #178905

    Purpose

    dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

    Depositor

    Constance Ciaudo

    Article

    Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

    Insert

    3'UTR mMov10 (Mov10 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    SV40 promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. psiCHECK2-mMOV10-3'UTR-MRE16-mut1

    Plasmid

    #178906

    Purpose

    dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

    Depositor

    Constance Ciaudo

    Article

    Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

    Insert

    3'UTR mMov10 (Mov10 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    CTG>AGT miR16-5p MRE1 mutated

    Promoter

    SV40 promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. psiCHECK2-mMOV10-3'UTR-MRE153-mut4

    Plasmid

    #178909

    Purpose

    dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

    Depositor

    Constance Ciaudo

    Article

    Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

    Insert

    3'UTR mMov10 (Mov10 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    TGC>CAT miR153-3p MRE mutated

    Promoter

    SV40 promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pX458_miR-290-295KO-gRNA3

    Plasmid

    #172711

    Purpose

    Cas9 with 5' gRNA for paired CRISPR KO of miR-290-295 cluster

    Depositor

    Constance Ciaudo

    Article

    Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

    Insert

    miR-290-295
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TFAP4_BHLH_5-1

    Plasmid

    #185054

    Purpose

    Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2

    Depositor

    Constance Ciaudo

    Article

    Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

    Insert

    TFAP4_5_1_gRNA (Tcfap4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TFAP4_BHLH_3-2

    Plasmid

    #185055

    Purpose

    Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_5-1 or TFAP4_BHLH_5-2

    Depositor

    Constance Ciaudo

    Article

    Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

    Insert

    TFAP4_3_2_gRNA (Tcfap4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge