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pYPQAT09

Plasmid

#223381

Purpose

T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

2x35s-SpRY-AtU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-5

Plasmid

#124985

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-5 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-5 TCRa P2A TCRb

Use

retroviral

Expression

Mammalian

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-pm-iATPSnFR1.0

Plasmid

#102552

Purpose

expresses membrane displayed ATP sensor in astrocytes

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Use

AAV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAG1243 NES-ECFP-FIREmate

Plasmid

#205169

Purpose

Expresses NES-ECFP-FIREmate in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

NES-ECFP-FIREmate

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pH6HTC_CCDC124-Halo

Plasmid

#175328

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

CCDC124 (CCDC124 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-4

Plasmid

#124984

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-4 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-4 TCRa P2A TCRb

Use

retroviral

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBR_attB(bxb)_ccdB_lox_v2

Plasmid

#198040

Purpose

Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQAT05

Plasmid

#223377

Purpose

T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-SpCas9-OsU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pQP-iRIS

Plasmid

#102584

Purpose

Regulation of protein localization (near-infrared-blue-light-inducible shuttle)

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

Insert

BphP1-mVenus-CAAX-IRES2-NES-mCherry-QPAS1-AsLOV2cNLS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAG1237 FIREmate-ECFP-Fis1

Plasmid

#205173

Purpose

Expresses FIREmate-ECFP-Fis1 in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

FIREmate-ECFP-Fis1

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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BFP dest clone

Plasmid

#71825

Purpose

Lentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression)

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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hPDI1_18-508_WT_pFLAG-CMV1

Plasmid

#31382

Depositor

David Ron

Article

Zito et al Mol Cell. 2010 Dec 10. 40(5):787-97.

Insert

hPDI1 (P4HB Human)

Tags

FLAG M2

Expression

Mammalian

Available Since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only

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CFP-CaaX

Plasmid

#155232

Purpose

membane marker

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

CFP C-term tagged with the CaaX sequence derived from Kras4b. Membrane control

Tags

CFP

Expression

Mammalian

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-3HaloTag

Plasmid

#179828

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3x HaloTag

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pC1-CMV-DAAO-NES

Plasmid

#141107

Purpose

Expresses DAAO with nuclear export signal in mammalian cells

Depositor

Vsevolod Belousov

Article

Bogdanova et al Curr Protoc Chem Biol. 2017 Jun 19;9(2):

Insert

DAAO-NES

Tags

Nuclear export signal

Expression

Mammalian

Mutation

deleted DAAO amino acids 367-368

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRSV-p65

Plasmid

#106453

Purpose

expression of NF-kB p65

Depositor

Georg Weber

Article

He et al Eur J Biochem. 2003 May . 270(10):2174-8

Insert

NF-kB p65 (RELA Human)

Expression

Mammalian

Mutation

WT

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_C_ROS1

Plasmid

#187766

Purpose

MAC-tagged gene expression of human ROS1

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

ROS1 (ROS1 Human)

Expression

Mammalian

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-mNeon-TurboID

Plasmid

#186610

Purpose

Third generation lentiviral vector expressing mNeonGreen fused to V5-TurboID

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

mNeon-V5-TurboID

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

For reference see Branon et al., 2018

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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10TALE_Pmin_fLuc

Plasmid

#160663

Purpose

Expression of the firefly luciferase reporter upon binding of a transcriptional activator to target sites. The plasmid contains 10 copies of target sites for the TALEN1257 DNA-binding domain

Depositor

Roman Jerala

Article

Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

Insert

10TALE_Pmin_fLuc

Use

Luciferase

Expression

Mammalian

Promoter

minimal

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT3L

Plasmid

#35523

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3L (DNMT3L Human)

Tags

Myc

Expression

Mammalian

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only

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