We narrowed to 15,731 results for: LEA

1. pLentiCRISPRv2_cGAS_gRNA_3

   Plasmid

   #235531

   Purpose

   gRNA against human cGAS

   Depositor

   Nicolas Manel

   Article

   Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

   Insert

   cGAS (CGAS Human)
   

   Use

   Lentiviral

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-AKAP79-AKAR4

   Plasmid

   #181957

   Purpose

   Genetically encoded FRET-based PKA activity reporter fused to AKAP79.

   Depositor

   Jin Zhang

   Article

   Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

   Insert

   AKAP79-AKAR4 (AKAP5 Human)
   

   Tags

   AKAP79, Cerulean, HIS tag, and cpVenus(E172)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. LCV2 V-1

   Plasmid

   #84804

   Purpose

   Cas9 with sgRNA targeting Exon 1 of VEGF-A

   Depositor

   Glenn Yiu

   Article

   Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

   Insert

   gRNA targeting VEGF-A (VEGFA Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. LCV2 V-3

   Plasmid

   #84806

   Purpose

   Cas9 with sgRNA targeting Exon 1 of VEGF-A

   Depositor

   Glenn Yiu

   Article

   Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

   Insert

   gRNA targeting VEGF-A (VEGFA Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDisplay-GRAB_CRFmut

   Plasmid

   #208656

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 TAK1/F

   Plasmid

   #44161

   Depositor

   Xin Lin

   Article

   Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

   Insert

   Tak1 (Map3k7 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Oct. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)-RasAR-NES

   Plasmid

   #205567

   Purpose

   Genetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.

   Depositor

   Jin Zhang

   Article

   Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

   Insert

   RasAR-NES (RAF1 Human, Synthetic)
   

   Tags

   Nuclear export signal (NES)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-N1-EXO1b

   Plasmid

   #111621

   Purpose

   Expression of human WT Exonuclease 1 (EXO1) tagged with mCherry in mammalian cells

   Depositor

   Marco Muzi-Falconi

   Article

   Sertic et al Mol Cell. 2018 Apr 5;70(1):34-47.e4. doi

   Insert

   Exonuclease 1b (EXO1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT/TO-3*Flag-APEX2 C-term PML

   Plasmid

   #187583

   Purpose

   Express FLAG epitope and APEX2-tagged PML fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   PML (PML Human)
   

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-EF1a-EGFP-ERGIC53-IRES-Puromycin

    Plasmid

    #134859

    Purpose

    Expresses EGFP-tagged ERGIC marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    ERGIC53 (Lman1 Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pELPS-eDHFR-YFP-T2A-Renilla

    Plasmid

    #193253

    Purpose

    Triple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase

    Depositor

    Mark Sellmyer

    Article

    Sellmyer et al Mol Ther. 2020 Jan 8;28(1):42-51. doi: 1

    Insert

    The eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
    

    Use

    Lentiviral

    Tags

    Renilla luciferase (rLuc) and yellow fluorescent …

    Mutation

    None

    Promoter

    EF1a

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST-DHFR F[1,2]-C (TRP1)

    Plasmid

    #177795

    Purpose

    Gateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

    Type

    Empty backbone

    Tags

    DHFR F[1,2]

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EF1a-mCherry-IRES-Flpo

    Plasmid

    #55634

    Purpose

    Expresses Flpo in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV1

    Inserts

    mCherry
    Flpo
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1a and Ef1a/IRES

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIA33

    Plasmid

    #120803

    Purpose

    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

    Inserts

    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Base-pairing region targets red fluorescent prote…

    Promoter

    Pgdh and Pxyl

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pk7-GR-GFP

    Plasmid

    #15534

    Depositor

    Ian Macara

    Article

    Carey et al J Cell Biol. 1996 Jun . 133(5):985-96.

    Insert

    Glucocorticoid receptor (NR3C1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The glucocorticoid receptor was cloned into the X…

    Available Since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTarget

    Plasmid

    #214052

    Purpose

    Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    Target sequence
    

    Use

    Target rna plasmid

    Expression

    Bacterial

    Promoter

    trc promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHochTypeIII

    Plasmid

    #214039

    Purpose

    Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    Cas10
    

    Use

    CRISPR

    Mutation

    WT

    Promoter

    T7 promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRN120

    Plasmid

    #160696

    Purpose

    BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminator

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Inserts

    CmYLCV::Luc+::AtHSP
    Nos::WUS2::PinII
    AtUbi10::STM::35S
    

    Use

    Luciferase; Gemini viral replicon, plant developm…

    Promoter

    AtUbi10, CmYLCV, and Nos

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. EF.delmFasL.CMV.GFP

    Plasmid

    #17620

    Depositor

    Linzhao Cheng

    Article

    Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

    Insert

    delFasL (Fasl Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Noncleavable mouse delFasL . Deleted the exon 2 r…

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn Con/Fon EYFP

    Plasmid

    #55650

    Purpose

    Cre-on/Flp-on EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV8

    Insert

    eYFP with introns
    

    Use

    AAV; Cre on/flp on eyfp

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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