We narrowed to 15,498 results for: LIS

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gRNAy

Plasmid

#168294

Purpose

Expresses gRNA targeting the yellow gene in Drosophila

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

U6.3-gRNA[y]-scaffold

Expression

Insect

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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Blat-BSD

Plasmid

#163791

Purpose

The plasmid expresses human codon-optimized BlatCas9, gRNA expression elements and blasticidin resistence gene.

Depositor

Yongming Wang

Article

Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 UPA-CARD WT

Plasmid

#164021

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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p33Fn

Plasmid

#158155

Purpose

Expression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Mutation

Rice codon optimized

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMGC

Plasmid

#112812

Purpose

PCR template vector for amplifying gRNA(F+E)-OstRNA fragment

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Insert

gRNAcore(F+E)-tRNA

Expression

Bacterial

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pST1374-H840A-GCN4

Plasmid

#113027

Purpose

Expresses H840A-GCN4 in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

H840A-GCN4 (GCN4 Budding Yeast, S. pyogenes)

Expression

Mammalian

Mutation

D839A, H840A, N863A

Promoter

CMV

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-Flag-mPRMT7-GFP

Plasmid

#87104

Purpose

Express mouse PRMT7 with Flag-tag in mammalian cells

Depositor

Min Gyu Lee

Article

Lee et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

Protein arginine methyltransferase 7 (Prmt7 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

EF1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ150-cul4

Plasmid

#80353

Purpose

Pmex-5:GFP::H2B::PEST::cul-4 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::cul-4 3'UTR

Expression

Worm

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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p-miR-Report + MT miR-181 BS in NLK 3UTR

Plasmid

#22791

Depositor

Xin Wang

Article

Ji et al Hepatology. 2009 Aug . 50(2):472-80.

Insert

Mutant miR-181 Binding Site in Nemo-like kinase 3 Untranslated Region (NLK Human)

Use

Luciferase

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pCgVchCAST-crtYf

Plasmid

#203816

Purpose

Expression of CRISPR-associated transposases, shuttle vector for Corynebacterium glutamicum / E. coli

Depositor

Sheng Yang

Article

Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

Insert

VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(crtYf)

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pXJ218-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-mtagBFP2-KASH-PB5'IR

Plasmid

#213480

Purpose

AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

mtagBFP-KASH
U6-gRNA-directcapture

Use

AAV

Expression

Mammalian

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_STRAP:1-350

Plasmid

#211139

Purpose

Mammalian expression of full-length human STRAP. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

STRAP:M1-A350 (STRAP Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_RBBP7:1-425

Plasmid

#211113

Purpose

Mammalian expression of full-length human RBBP7. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RBBP7:M1-S425 (RBBP7 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_CBFA2T3:1-615

Plasmid

#211070

Purpose

Mammalian expression of full-length human CBFA2T3. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CBFA2T3:M1-R615 (CBFA2T3 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

not annotated isoform

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_FBXW7:1-707

Plasmid

#211104

Purpose

Mammalian expression of full-length human FBXW7. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

FBXW7:M1-K707 (FBXW7 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHTC-HaloTag_RAB7A:1-207

Plasmid

#211096

Purpose

Mammalian expression of full-length human RAB7A. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RAB7A:M1-C207 (RAB7A Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNLF1-C_XPO1:1-1071

Plasmid

#211088

Purpose

Mammalian expression of full-length human XPO1. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

XPO1:M1-D1071 (XPO1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_PRPF19:1-504

Plasmid

#211132

Purpose

Mammalian expression of full-length human PRPF19. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PRPF19:M1-L504 (PRPF19 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_RACK1:1-317

Plasmid

#211110

Purpose

Mammalian expression of full-length human RACK1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RACK1:M1-R317 (RACK1 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_CRBN:1-442

Plasmid

#211067

Purpose

Mammalian expression of full-length human CRBN the C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CRBN:M1-L442 (CRBN Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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