We narrowed to 7,572 results for: Lif

1. pBAD-Padron2

   Plasmid

   #172355

   Purpose

   Expresses the reversibly switchable fluorescent protein Padron2 in bacterial cells

   Depositor

   Stefan Jakobs

   Article

   Konen et al ACS Nano. 2021 May 21. doi: 10.1021/acsn

   Insert

   Padron2
   

   Use

   Tags

   6xHisTag (N terminal on backbone)

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-FLEX-FLIM-AKAR

   Plasmid

   #60445

   Purpose

   Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backbone

   Depositor

   Bernardo Sabatini

   Article

   Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

   Insert

   FLIM-AKAR
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRE-Tight-FLAG-CYLD-WT

   Plasmid

   #60030

   Purpose

   WT CYLD under control of Tet promoter with N-terminal FLAG tag

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   Cylindromatosis (CYLD Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Nov. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-ihSyn1-tTA

   Plasmid

   #99120

   Purpose

   An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTA

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Has Service

   AAV1

   Insert

   tTA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   *(see below)

   Promoter

   ihSyn1

   Available since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Sumo3 PolQM1

   Plasmid

   #78462

   Purpose

   Inducible expression in E. coli

   Depositor

   Sylvie Doublie, Susan Wallace

   Article

   Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

   Insert

   Polymerase theta (POLQ Human)
   

   Use

   Tags

   His and SUMO3

   Expression

   Bacterial

   Mutation

   delta 1-1791; Q2513R (please see depositor commen…

   Promoter

   T7

   Available since

   July 28, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pGEX-5X-1-Full-Length-MePCE

   Plasmid

   #113539

   Purpose

   Produces an N-terminal GST-tagged full length MePCE protein.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   MePCE (MEPCE Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-CYLD-STOP

   Plasmid

   #60077

   Purpose

   N-terminal GFP tagged CYLD with stop codon, driven by CMV promoter

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   Cylindromatosis (CYLD Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. HRAS

   Plasmid

   #55653

   Purpose

   expression of mouse HRAS

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   HRAS (Hras Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVTREGCaMP6f

   Plasmid

   #97410

   Purpose

   Amplified expression of GCaMP6f in combination with TET driver

   Depositor

   Tetsuo Yamamori

   Article

   Sadakane et al Cell Rep. 2015 Dec 1;13(9):1989-99. doi:

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3

   Available since

   Jan. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD IFP1.4

    Plasmid

    #91775

    Purpose

    Bacterial expression of a fluorescent protein, Infrared Fluorescent Protein 1.4 + Heme oxygenase-1 (IFP1.4)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shu et al Science. 2009 May 8;324(5928):804-7. doi

    Insert

    Infrared Fluorescent Protein 1.4 + Heme oxygenase-1
    

    Use

    Tags

    Hexa-Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWPT-/GCCACC-mEGFP-IRES-mCherry

    Plasmid

    #49235

    Purpose

    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-short

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRX-IB-mRuby3-FTH1

    Plasmid

    #192804

    Purpose

    Stable expression of mRuby3-FTH1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    FTH1 (FTH1 Human)
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Hsp70-mplum

    Plasmid

    #45016

    Depositor

    Anna Planas

    Article

    de la Rosa et al Eur J Nucl Med Mol Imaging. 2013 Feb;40(

    Insert

    mPlum
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse Hspa1b

    Available since

    May 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR P4-P1R-ECFP

    Plasmid

    #48352

    Purpose

    Contributes the coding sequence for ECFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    ECFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Contains a Kozak sequence. Does not contain a sto…

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-FLAG-VSVG

    Plasmid

    #80606

    Purpose

    Mammalian expression vector that expresses a VSV-G protein with a FLAG tag near the N-terminus of the protein

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    VSV-G
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-CaMPARI V398D

    Plasmid

    #60786

    Purpose

    Expresses CaMPARI V398D in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

    Depositor

    Loren Looger, Eric Schreiter

    Article

    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

    Insert

    CaMPARI V398D
    

    Use

    Tags

    Nuclear Export Signal

    Expression

    Mammalian

    Mutation

    V398D

    Promoter

    CMV

    Available since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR P2R-P3-SV40 polyA signal

    Plasmid

    #48627

    Purpose

    Contributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    SV40 early polyadenylation signal cassette
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-SPA2G231V

    Plasmid

    #42079

    Depositor

    Christine Garcia

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

    Insert

    Surfactant Protein -A2 (SFTPA2 Human)
    

    Use

    Lentiviral; Plenti

    Tags

    V5

    Expression

    Bacterial and Mammalian

    Mutation

    Changed Glycine 231 to Valine

    Promoter

    CMV

    Available since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pMAL-MBP-Cdc42-Q61L

    Plasmid

    #214288

    Purpose

    Express constitutively active Cdc42 fused with MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Constitutively active Cdc42 (CDC42 Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Cdc42-Q61L

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDest27-mBIN1+13+17

    Plasmid

    #61097

    Purpose

    Express N-terminal GST tagged-mouse BIN1+13+17 in mammalian cells

    Depositor

    Robin Shaw

    Article

    Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

    Insert

    mouse BIN1 with exon 13 and 17, excluding exon 7, 11, 14-16
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only