9,059 results

Showing: 6861 - 6880 of 9059 results

Results per page:

Sort results by:

pPB-CAG-hCas3

Plasmid

#134920

Purpose

Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

Depositor

Tomoji Mashimo

Article

Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

Insert

Cas3 with bpNLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-ymNeongreen-SpHis5

Plasmid

#125704

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with SpHIS5 selection

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeonGreen

Tags

ymNeonGreen

Expression

Yeast

Available Since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PB_tre_dCas9_VP160

Plasmid

#126031

Purpose

PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

dCas9 fusion with VP160
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

EF1 alpha and TRE

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-PDGFRA

Plasmid

#116769

Purpose

Lentiviral expression of PDGFRA

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PDGFRA (PDGFRA Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_mCherry_P2A_Hygro_Barcode

Plasmid

#120426

Purpose

Barcoded lentiviral vector to express mCherry in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

mCherry

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSGDlucV3.0

Plasmid

#119760

Purpose

dual luciferase with FMDV StopGo sequences flanking MCS

Depositor

John Atkins

Article

Loughran et al Mol Cell. 2022 Oct 6;82(19):3745-3749.e2

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Promoter

SV40 and T7

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIH-TOMM20-Halo

Plasmid

#111626

Purpose

Fusion protein used to visualise mitochondria upon the addition of Halo specific dye, has hygromycin selection

Depositor

Benjamin Kile

Article

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

TOMM20 (Tomm20 Mouse)

Use

Retroviral

Tags

HaloTag

Expression

Mammalian

Promoter

MSCV

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLNCX2-mEGFP-TSG101

Plasmid

#116925

Purpose

expresses mEGFP-TSG101 in mammalian cells

Depositor

Sanford Simon

Article

Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

Insert

TSG101 (TSG101 Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI

Plasmid

#115268

Purpose

Expresses EiCsm6 from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

EiCsm6

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK231 (AAVS1 CMV-MCS-PURO)

Plasmid

#105924

Purpose

Donor for integration at the human AAVS1 locus

Depositor

Masato Kanemaki

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiPGK Hygro DEST H2B-mRuby2

Plasmid

#90236

Purpose

Lentiviral vector to express H2B-mRuby2 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
SunTagng22aa

Plasmid

#106438

Purpose

Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSN067

Plasmid

#101748

Purpose

Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.

Depositor

Rene Verwaal

Article

Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

Inserts

Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
KanMX marker expression cassette.

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Promoter

Heterologous TEF1 promoter from A. gossypii. and …

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-ABE7.8

Plasmid

#102917

Purpose

expresses ABE7.8 in mammalian cells

Depositor

David Liu

Article

Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

Insert

ABE7.8

Tags

NLS

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
sg2.02 16x(MS2) MUC4.1

Plasmid

#101154

Purpose

gRNA with 16 MCP binding site

Depositor

Mazhar Adli

Article

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

MUC4 (MUC4 Human)

Use

CRISPR and Lentiviral

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_B2103

Plasmid

#91061

Purpose

Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers

Use

CRISPR

Promoter

CmYLCV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-EN479

Plasmid

#86234

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nickase and sgRNA against mouse rosa26 locus

Use

Mouse Targeting

Expression

Mammalian

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TCRalpha_E2A_SP-SNAPf-TCRbeta_P2A_CD3e_T2A_CD3zeta

Plasmid

#89347

Purpose

2nd generation lentiviral vector which expresses SNAP tagged TCRbeta and other TCR subunits (Jurkat alleles) separated via 2A sequences

Depositor

Ron Vale

Article

Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

Insert

Jurkat TCRalpha/beta alleles and CD3e and CD3z

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-NSP1SA11

Plasmid

#89168

Purpose

Express Rotavirus SA11 NSP1 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP1

Expression

Mammalian

Promoter

T7 promotor

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-BiFC

Plasmid

#87856

Purpose

pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Inserts

Encodes for MCS & C-term half of mVenus
Encodes for MCS & N-term half of mVenus

Tags

BiFC C-terminal fragment

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge