We narrowed to 9,391 results for: BLI

1. pCLIPf-Cox8A Control Plasmid

   Plasmid

   #101130

   Purpose

   Expression of CLIP-Tagged Cox8A in Mammalian cells

   Depositor

   New England Biolabs, Ana Egana

   Insert

   Cox8A (COX8A Human)
   

   Tags

   CLIP-tag (CLIPf)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pelBHisTEV

   Plasmid

   #157741

   Purpose

   empty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sites

   Depositor

   Debra Hansen

   Type

   Empty backbone

   Tags

   PelB signal sequence + His10-tag + TEV protease c…

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pB6-Gfap-T2A-tdTomato-FNF-DTA

   Plasmid

   #140271

   Purpose

   B6J Gfap-T2A-tdTomato allele targeting vector, low copy number

   Depositor

   Mario Capecchi

   Insert

   Gfap (Gfap Mouse)
   

   Use

   Mouse Targeting

   Tags

   C-terminal fusion of T2A-tdTomato

   Expression

   Mammalian

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGFP Cas

   Plasmid

   #50729

   Purpose

   mammalian expression of GFP-Cas. Note-This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

   Depositor

   Kenneth Yamada

   Insert

   Cas (Bcar1 Rat)
   

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine

   Plasmid

   #50470

   Purpose

   Gq-coupled hM3D-IRES-mCitrine under the control of GFAP promoter

   Depositor

   Bryan Roth

   Insert

   hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
   

   Use

   AAV

   Tags

   HA

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   GFAP

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQTEV-TAGLN2

   Plasmid

   #31569

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   transgelin 2 (TAGLN2 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-hSyn-GluA2-scFv-mNeon-KDEL

   Plasmid

   #230057

   Purpose

   AAV plasmid that expresses a a single chain variable fragment (scFv) for anti- GluA2 with a C-terminal mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.

   Depositor

   Matthew Kennedy

   Insert

   Anti-GluA2 single chain variable fragment (scFv)
   

   Use

   AAV

   Tags

   ER retrieval motif (KDEL) and mNeon

   Expression

   Mammalian

   Promoter

   human synapsin

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PRDX6A-c001

   Plasmid

   #175474

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.

   Depositor

   Opher Gileadi

   Insert

   PRDX6 (PRDX6 Human)
   

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Mutation

   See Depositor Comments

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HECTD1

   Plasmid

   #32860

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HECTD1 (HECTD1 Human)
   

   Tags

   His (with TEV cleavage)

   Expression

   Bacterial

   Mutation

   amino acids 1879-1966 only

   Available Since

   Jan. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pQE80L SpyTag-ELP-SpyTag

    Plasmid

    #112634

    Purpose

    Two SpyTags linked by an elastin-like polypeptide for hydrogel formation

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    SpyTag-ELP-SpyTag
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    central cysteine

    Promoter

    T5

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pALS3-Ma-sfGFP-TAG150

    Plasmid

    #212121

    Purpose

    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    sfGFP-150TAG
    M. alvus Pyl-tRNA(6)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    N150TAG

    Promoter

    araC and lpp

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBK-Ma pylRS nitroY/haloY-F5

    Plasmid

    #212125

    Purpose

    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.

    Depositor

    Ryan Mehl

    Insert

    M. alvus PylRS 3-NY/HaloY F5
    

    Expression

    Bacterial

    Mutation

    CTG125CTT, N166A V168S, A223C, W239R

    Promoter

    GlnS (constitutive)

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AICSDP-117:POLR2A-mEGFP

    Plasmid

    #164500

    Purpose

    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A

    Depositor

    Allen Institute for Cell Science

    Insert

    POLR2A Homology Arms with mEGFP-linker (POLR2A Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Feb. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. psd44-G12QL

    Plasmid

    #46825

    Purpose

    expression of mutant G protein alpha 12 in mammalian cells

    Depositor

    Agnese Mariotti

    Insert

    GNA12 (GNA12 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Q231L (the mutation reduces GTPase activity resul…

    Promoter

    PGK

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG416-GAL-FUS-G156E

    Plasmid

    #181716

    Purpose

    Galactose inducible expression of FUS-G156E

    Depositor

    Alejandro Chavez

    Insert

    FUS (FUS Human)
    

    Expression

    Yeast

    Mutation

    G156E

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Hs.KRAS4a

    Plasmid

    #83166

    Purpose

    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid

    #50467

    Purpose

    Gi-coupled hM4D-IRES-mCitrine under the control of CaMKIIa promoter

    Depositor

    Bryan Roth

    Has Service

    AAV2 and AAV8

    Insert

    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    CaMKIIa

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid

    #50471

    Purpose

    Gi-coupled hM4D-IRES-mCitrine under the control of GFAP promoter

    Depositor

    Bryan Roth

    Insert

    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    GFAP

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJCX3-ACTG2_R257C

    Plasmid

    #228911

    Purpose

    Expresses mutant R257C smooth muscle actin gamma-2 in human cells

    Depositor

    Roberto Dominguez

    Insert

    Actin gamma 2, smooth muscle (ACTG2 Human)
    

    Tags

    HIS-FLAG-TEV

    Expression

    Bacterial and Mammalian

    Mutation

    R257C

    Promoter

    CMV

    Available Since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJCX3-ACTG2_WT

    Plasmid

    #228910

    Purpose

    Expresses wildtype smooth muscle actin gamma-2 in human cells

    Depositor

    Roberto Dominguez

    Insert

    Actin gamma 2, smooth muscle (ACTG2 Human)
    

    Tags

    HIS-FLAG-TEV

    Expression

    Bacterial and Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge