We narrowed to 8,430 results for: BLI

1. pQTEV-PRKRA

   Plasmid

   #31571

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

   Insert

   protein kinase, interferon-inducible double stranded RNA dependent activator (PRKRA Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

   Plasmid

   #174140

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

   Promoter

   CMV and T7

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVRC8400-SARS-CoV-2-RBD-BA.4/5-AVI

   Plasmid

   #192114

   Purpose

   Expresses SARS-CoV-2 RBD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Insert

   SARS-CoV-2-RBD-BA.4/5 (S )
   

   Use

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   G339D, S371F, S373P, S375F, T376A, D405N, R408S, …

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFP.R371

   Plasmid

   #138241

   Purpose

   Expresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(NGK).MP-M-DnaB*.(TEL)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   P(araBAD) and iPC

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO/Flag-tGFP-HuR.wt

   Plasmid

   #110376

   Purpose

   Mammalian expression of turboGFP-HuR (wild type) with FLAG tag, Flp-In T-Rex system

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAV like RNA binding protein 1 (ELAVL1 Human)
   

   Use

   Tags

   FLAG and tGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-hygro-SNAP-FLAG-CD59

   Plasmid

   #50379

   Purpose

   Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammalian cells

   Depositor

   Reika Watanabe

   Insert

   SNAP-FLAG-tagged CD59 (CD59 Human)
   

   Use

   Tags

   FLAG and SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   SR alpha

   Available since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

7. SMARCA4

   Plasmid

   #39116

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   SMARCA4 (SMARCA4 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pSB700 HL1gR4

   Plasmid

   #124450

   Purpose

   Expresses gRNA HL1 gR4 in the pSB700 backbone

   Depositor

   George Church

   Article

   Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

   Insert

   HL1 gR4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUAS-kkv-R896K::NG

   Plasmid

   #138957

   Purpose

   Used to generate a transgene that will express an inactive fluorescent Chitin Synthase in Drosophila using Gal4

   Depositor

   Paul Adler

   Article

   Adler et al bioRxiv 2020

   Insert

   kkv-R896K::NG (kkv Fly)
   

   Use

   Tags

   Neon Green fused to C terminus of Kkv

   Expression

   Insect

   Mutation

   R896K

   Promoter

   UAS

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUSE-rabbitFc1-IL2ss-VHH(B12)-His-Myc

    Plasmid

    #209837

    Purpose

    Plasmid coding for a nanobody against FGFR3 (mus musculus) with x6 HisTag, x3 myc, and Fabbit FC gamma 1

    Depositor

    Tim Brown

    Inserts

    IL2-ss
    VHH (B12 variant)
    Rabbit Fc Gamma 1
    

    Use

    Tags

    x3 MycTag and x6 HisTag

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1-HTLV promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-hDAGLb-V5

    Plasmid

    #87675

    Purpose

    Expresses human DAGLb protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal

    Depositor

    Pat Doherty

    Insert

    Human Diacylglycerol Lipase Beta (DAGLB Human)
    

    Use

    Tags

    HA and V5 tag

    Expression

    Mammalian

    Mutation

    Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…

    Promoter

    T7

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. ATM_N_FS_v2_Donor

    Plasmid

    #214815

    Purpose

    N-terminal tagging of human ATM with 3xFLAG_Twin_Strep

    Depositor

    Yannick Doyon

    Insert

    3xFLAG_2xStrep for tagging ATM at the N-terminus (ATM Synthetic, Human)
    

    Use

    CRISPR

    Tags

    3xFLAG_2xStrep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti-CMV-TFAM-BioID2-IRES-Puro

    Plasmid

    #211465

    Purpose

    Lentivirus backbone expressing human TFAM fused with BioID2

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    TFAM-BioID2
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. wt HA-dynamin 1 pUHD10-3

    Plasmid

    #34688

    Depositor

    Sandra Schmid

    Article

    Damke et al J Cell Biol. 1995 Oct;131(1):69-80.

    Insert

    dynamin 1 (DNM1L Human)
    

    Use

    Tags

    HA (variant)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pFP.R400

    Plasmid

    #138175

    Purpose

    Expresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(GGK).NpuSsp*.(CWN)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

    Plasmid

    #117070

    Purpose

    Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection

    Depositor

    Kim Failor

    Inserts

    Cas9
    SacB
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pL1P3 ZmUbiP:GRF-GIF:NosT

    Plasmid

    #198047

    Purpose

    MoClo Golden Gate Level 1 Position 3. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Cristobal Uauy

    Insert

    ZmUbiP::TaGRF4-GIF1::NosT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Zea mays (maize) ubiquitin promoter

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO

    Plasmid

    #68405

    Purpose

    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter

    Depositor

    Jannik Elverløv-Jakobsen

    Inserts

    3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
    FlpO
    

    Use

    CRISPR

    Tags

    mTurquoise2 (BFP)

    Expression

    Mammalian

    Mutation

    Promoter

    Synthetic Probasin ARRx2 and U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGAGmEos2-N1

    Plasmid

    #46372

    Depositor

    Suliana Manley

    Article

    Gunzenhauser et al Nano Lett. 2012 Sep 12;12(9):4705-10. do

    Insert

    Gag
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Rev independentHXB2 Gag sequence

    Promoter

    CMV

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. CXXC1

    Plasmid

    #32045

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CXXC1 (CXXC1 Human)
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    CXXC domain (amino acids residues 161-222)

    Promoter

    Available since

    Jan. 3, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits