We narrowed to 7,572 results for: Lif

1. Cav2.2 HA pcDNA3

   Plasmid

   #206051

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

   Depositor

   Annette Dolphin

   Article

   Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFlc245

   Plasmid

   #101021

   Purpose

   fluorescent, functional, glutamate receptor subunit

   Depositor

   Thomas Hughes

   Article

   Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

   Insert

   GluR 1 (Gria1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   fluorescent protein inserted into subunit

   Promoter

   CMV

   Available since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST14-ARL3

   Plasmid

   #67376

   Purpose

   Expression of native hArl3 in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL3 (ARL3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. L-GARS-2

   Plasmid

   #166531

   Purpose

   scFv of a human scaffold targeting Glycyl-tRNA synthetase. Antigen coverage aa 55-739 of 739

   Depositor

   Susanne Gräslund

   Article

   Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

   Insert

   single chain-Fv, scFv
   

   Use

   Recombinant antibody expression

   Tags

   3xFLAG, His6 and OmpA leader sequence

   Expression

   Bacterial

   Mutation

   Promoter

   LacZ

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Myc-CYLD-C601A

   Plasmid

   #60027

   Purpose

   Myc-tagged CYLD catalytic inactive (C601A) expression

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   Cylindromatosis (CYLD Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Cysteine 601 changed to Alanine

   Promoter

   CMV

   Available since

   Nov. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJRoC30_Th3

   Plasmid

   #188064

   Purpose

   PROSS-designed high-redox potential laccase from Trametes hirsuta

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   PROSS-designed high-redox potential laccase from Trametes hirsuta
   

   Use

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 mutations, described in publication

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJRoC30_Tv9nL

   Plasmid

   #188069

   Purpose

   PROSS-designed high-redox potential laccase from Trametes versicolor

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   PROSS-designed high-redox potential laccase from Trametes versicolor
   

   Use

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   79 PROSS mutations, described in publication

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGG-NES-TurboID-GFP

   Plasmid

   #209401

   Purpose

   Transiently expressing and visualizing the subcellular localization of TurboID fused with a nuclear export signal (NES) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   NES-3XHA-TurboID
   

   Use

   Tags

   GFP

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME GFP-PCNA

   Plasmid

   #105977

   Purpose

   entry clone for gateway cloning, cell cycle marker proliferative cell nuclear antigen PCNA

   Depositor

   Caren Norden

   Article

   Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

   Insert

   PCNA (PCNA Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGS1021

    Plasmid

    #59575

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection marker

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    Twin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
    

    Use

    E. coli cloning vector

    Tags

    Twin-Strep tag

    Expression

    Mutation

    Promoter

    None

    Available since

    Aug. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS ColI alpha2

    Plasmid

    #19207

    Depositor

    Cliff Tabin

    Article

    Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

    Insert

    Alpha 2 type I collagen (COL1A2 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pDONR P2R-P3-mKate2

    Plasmid

    #48345

    Purpose

    Contributes the coding sequence for the mKate2 fluorescent protein as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    mKate2
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Contains a stop codon at the 3' end of the c…

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR P4-P1R-EYFP

    Plasmid

    #48350

    Purpose

    Contributes the coding sequence for EYFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    EYFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Contains a Kozak sequence. Does not contain a sto…

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR P2R-P3-ECFP

    Plasmid

    #48353

    Purpose

    Contributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    ECFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Contains a stop codon.

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMpGWB122

    Plasmid

    #68576

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS mEya1 (CT#671)

    Plasmid

    #13971

    Depositor

    Cliff Tabin

    Article

    Heanue et al Mech Dev. 2002 Feb . 111(1-2):75-87.

    Insert

    Eya1 (Eya1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

17. J-HARS-4

    Plasmid

    #166529

    Purpose

    scFv of a human scaffold targeting Histidyl-tRNA synthetase. Antigen coverage aa 1-509 of 509, full-length

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. L-SARS-7

    Plasmid

    #166527

    Purpose

    scFv of a human scaffold targeting Seryl-tRNA synthetase. Antigen coverage aa 1-514 of 514, full-length

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti.puro.MCV.ER.RAZ.2

    Plasmid

    #114382

    Purpose

    This lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=428 aa.

    Depositor

    Sudhir Krishna, Reety Arora

    Article

    Arora et al Microb Pathog. 2019 Dec;137:103778. doi:

    Insert

    MCV NCCR and tumor T antigen regions
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MCV NCCR (viral own promoter)

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAP03-act1p

    Plasmid

    #120233

    Purpose

    Carries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring.

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    promoter cassette containing act1p promoter and apramycin resistance gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    aac(3)IV (apramycin resistance gene)

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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