We narrowed to 63,610 results for: cell

1. Phage-PGK-Flag-HA-FKBP-T(WT)

   Plasmid

   #181734

   Purpose

   for lentiviral expression of brachyury ORF in mammalian cells

   Depositor

   Charles Lin

   Article

   Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

   Insert

   T (brachyury)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   a silent mutation in the PAM site of an sgRNAt ta…

   Promoter

   Available Since

   March 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pEF6-Acly(S455D)

   Plasmid

   #70768

   Purpose

   Expresses phosphomimetic mouse ATP-citrate lyase in mammalian cells

   Depositor

   Kathryn Wellen

   Article

   Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

   Insert

   ATP Citrate Lyase (Acly Mouse)
   

   Use

   Tags

   myc-epitope

   Expression

   Mammalian

   Mutation

   S455D

   Promoter

   EF1alpha

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Fucci(CA) hCdt1-iRFP hGeminin-iRFP

   Plasmid

   #190182

   Purpose

   Fluorescent reporter vector for visualization of G0 and other cell cycle phases.

   Depositor

   Toshiro Sato

   Article

   Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

   Inserts

   iRFP
   hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
   iRFP
   hGeminin(1/110)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF6-Acly(S455A)

   Plasmid

   #70767

   Purpose

   Expresses phosphomutant mouse ATP-citrate lyase in mammalian cells

   Depositor

   Kathryn Wellen

   Article

   Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

   Insert

   ATP Citrate Lyase (Acly Mouse)
   

   Use

   Tags

   myc-epitope

   Expression

   Mammalian

   Mutation

   S455A

   Promoter

   EF1alpha

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXS-IRES-Blast mAtg7

   Plasmid

   #173170

   Purpose

   Retroviral vector to express sgRNA resistant mouse Atg7

   Depositor

   Kivanc Birsoy

   Article

   Zhu et al Cell Metab. 2020 Oct 28. pii: S1550-4131

   Insert

   Autophagy Related 7 (Atg7 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Synonymous mutations at sgRNA sites

   Promoter

   MMLV

   Available Since

   Feb. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti.pFOXA2.GFP

   Plasmid

   #59404

   Purpose

   expresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

   Depositor

   Kai-Christian Sonntag

   Article

   Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

   Insert

   400 bp FOXA2 promoter, eGFP (FOXA2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pFOXA2

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-SIN-BX SpyCatcher003-(short)mCD80

   Plasmid

   #210567

   Purpose

   Expresses Spycatcher003 on the cell surface using mouse CD80 (short) hinge, transmembrane, and cytoplasmic domain

   Depositor

   Omer Dushek

   Article

   Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

   Insert

   Spycatcher003 fused to mouse CD80 (short hinge + transmembrane + cytoplasmic domain)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMP71-hPDL-1

   Plasmid

   #118631

   Purpose

   human PDL-1 coding sequence subcloned into pMP71 retroviral plasmid

   Depositor

   Sébastien Walchli

   Article

   Josefsson et al Cancer Immunol Res. 2019 Mar;7(3):355-36

   Insert

   hPDL-1 (CD274 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti.pFOXA2.5'.GFP

   Plasmid

   #59407

   Purpose

   expresses eGFP from an about 1350 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

   Depositor

   Kai-Christian Sonntag

   Article

   Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

   Insert

   approx. 1350 bp FOXA2 promoter, eGFP (FOXA2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   FOXA2

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc-mCh-Tub

    Plasmid

    #24288

    Depositor

    Ron Vale

    Article

    Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

    Insert

    Alpha-Tubulin (alphaTub84B Fly)
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5

    Plasmid

    #97044

    Purpose

    Expresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Ligand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet on

    Available Since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGAPTrap-mtagBFP2-IRESMuro

    Plasmid

    #82335

    Purpose

    Vector targeting the mtagBFP2 gene to the GAPDH locus of human cells. BFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    mtagBFP2-IRESMuro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR_PGK-hACE2

    Plasmid

    #161612

    Purpose

    Expression of human ACE2 in mammalian cells

    Depositor

    Brad Rosenberg

    Article

    Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT-EGFP-Actin5c

    Plasmid

    #15312

    Depositor

    Ron Vale

    Article

    Rogers et al J Cell Biol. 2003 Sep 15. 162(6):1079-88

    Insert

    Actin5c (Act5C Fly)
    

    Use

    Tags

    EGFP and His

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pGAPTrap-mCherry-IRESMygro

    Plasmid

    #82505

    Purpose

    Vector targeting the mCherry gene to the GAPDH locus of human cells. mCherry is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    mCherry-IRESMygro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGAPTrap-eGFP-IRESMygro

    Plasmid

    #82503

    Purpose

    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    eGFP-IRESMygro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. FUW TetO HA-AMPKa2 WT

    Plasmid

    #69826

    Purpose

    Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV promoter with tetracycline operator

    Available Since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. cmamxA2-GFP

    Plasmid

    #107197

    Purpose

    Expresses human annexin A2-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted

    Depositor

    Ursula Rescher, Volker Gerke

    Article

    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

    Insert

    cmannexin A2 (ANXA2 Human)
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    all type II Ca2+ binding sites deleted ( D161A, E…

    Promoter

    CMV

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-CRT-Fc FL

    Plasmid

    #221853

    Purpose

    Express human Calreticulin (full-length) with C-terminal human IgG1Fc tag in mammalian cell.

    Depositor

    Judy Lieberman

    Article

    Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

    Insert

    calreticulin (CALR Human)
    

    Use

    Tags

    Human IgG1Fc tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIG-747_CD19-28z_CAR_TFAP4

    Plasmid

    #207489

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4, CD19-28z_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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