We narrowed to 11,647 results for: emb
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Plasmid#222324PurposeSynchronize the trafficking of LIMP2 from the ER.DepositorInsertStreptavidin-KDEL and LIMP2 fused to SBP-EGFP (SCARB2 Human)
ExpressionMammalianPromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
mEosEM-E_Lamin A
Plasmid#226338PurposePhotoswitchable fluorescent protein for correlative light-electron microscopy; nuclear envelope-targeted mEosEM-E for mammalian expressionDepositorAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Tras heavy chain-GSG-SpyTag003
Plasmid#216292PurposeExpresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras FabDepositorInsertTras heavy chain-GSG-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 tag, Signal peptide, and SpyTag003ExpressionMammalianPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII
Plasmid#217897PurposeInsect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tagDepositorTags2xSTIIExpressionInsectAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-RAB10 T23N-WPRE-UbC-Emerald
Plasmid#203802PurposeLentiviral vector plasmid expressing human RAB10 mutation T23N (dominant negative mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HP1-ECFP-SNAP
Plasmid#187966Purposeexpresses HP1 with C-terminal tags ECFP and SNAPDepositorAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_mGFP-FGFR3-K650Q
Plasmid#191756PurposeExpression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650Q mutation (associated with HCH; target mutation: c.1948A>C; silent mutation added on purpose: c.1938C>T)DepositorInsertmGFP-FGFR3IIIc (FGFR3 Human)
TagsmGFPExpressionMammalianMutationc.1948A>C (p.K650Q) + silent mutation c.1938C&…Available sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A
Plasmid#182049Purposeexpression of Sox2 mutantsDepositorAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET3d-SF-DCTN1-TS
Plasmid#169375PurposeE. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.DepositorInsertdynactin subunit 1 isoform 1 (DCTN1 Human)
Tags6xHis Tag, SplitFlAsH Tag, and TwinStrep TagExpressionBacterialMutationPoint mutations: A139T, I304T, Q322R, T516A, E577…Available sinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 (1-645, Δ368-523)/pcDNA3.1
Plasmid#111456PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
ExpressionMammalianMutationKv7.4 (1-645, Δ368-523), C643AAvailable sinceMay 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBB0323-TEV-His12
Plasmid#137039Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66700.1
TagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTIGER_tdTomato::3xFLAG::dCrk
Plasmid#131138PurposeUASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.DepositorAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD-BirA-eCPX-AP(K10)
Plasmid#121908PurposeExpresses BirA and eCPX-AP(K10)DepositorInsertsTags6xHisExpressionBacterialMutationchanged lysine 10 in the AP to alaninePromoterT7 and pBAD promoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1C389S-HLx8
Plasmid#108138PurposeRecruitable inactive SAC1 with extended linkerDepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
ExpressionMammalianMutationSACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-mEGFP
Plasmid#105137PurposeYeast gene targetingDepositorInsertkanMX6-P3nmt1-mEGFP
Tags3nmt1-mEGFPExpressionBacterial and YeastMutationA206KPromoter3nmt1Available sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-NSF/T645A
Plasmid#74936PurposeMammalian expression of human NSF mutant T645A (mutation in the D2 domain of the protein)DepositorInsertNSF (NSF Human)
TagsFLAGExpressionMammalianMutationT645A (mutation in the D2 domain of the protein)PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-NSF/S647A
Plasmid#74938PurposeMammalian expression of human NSF mutant S647A (mutation in the D2 domain of the protein)DepositorInsertNSF (NSF Human)
TagsFLAGExpressionMammalianMutationS647A (mutation in the D2 domain of the protein)PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43-YA/VD-Myc
Plasmid#60702Purposeretroviral expression of Myc tagged rat Cx43 YA/VD mutantDepositorInsertCx43 (Gja1 Rat)
UseRetroviralTagsMycExpressionMammalianMutationYA/VD (Y286A/V289D)PromoterLTRAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
PR594-His-bio
Plasmid#52332PurposeExpresses full-length Neuron-specific protein family member 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.DepositorInsertPR594 (NSG1 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
RP132 pUAST EGFP:Or43a
Plasmid#59523PurposeN-terminal fusion of EGFP to Drosophila melanogaster Or43aDepositorAvailable sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only