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pEQ1180

Plasmid

#83805

Purpose

express human cytomegalovirus TRS1 with a C-terminal 6X his tag in eukaryotic cells

Depositor

Adam Geballe

Article

Child et al J Virol. 2012 Apr;86(7):3880-9. doi: 10.

Insert

TRS1

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pMXs-C9orf114-flag

Plasmid

#70647

Purpose

Expresses C9orf114 with FLAG-tag epitope.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

C9orf114 (SPOUT1 Human)

Use

Retroviral

Tags

3X FLAG

Expression

Mammalian

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
Nano-lantern-Actin/pEGFP-C1

Plasmid

#51972

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to actin

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Tags

Actin

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pQStrep2-PIR

Plasmid

#31570

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

pirin (PIR Human)

Tags

His and StrepII-tag

Expression

Bacterial

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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EPHA8

Plasmid

#25343

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

epha8.0603.0909 (EPHA8 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pMOR256-17-ISH

Plasmid

#15528

Depositor

Peter Mombaerts

Article

Fuss et al Cell. 2007 Jul 27. 130(2):373-84.

Insert

MOR256-17 (Olfr15 Mouse)

Use

For riboprobe, in situ hybridization

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA HA DUSP6

Plasmid

#236430

Purpose

transient overexpression of DUSP6 in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Cell Rep. 2021 Oct 19;37(3):109870. doi:

Insert

DUSP6 (DUSP6 Human)

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pME-MCS (JDW 455)

Plasmid

#229832

Purpose

A gateway compatible middle entry clone containing an MCS (mutiple cloning site).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attL1/L2 flanked MCS

Use

Gateway Cloning

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shYAP1-1

Plasmid

#193692

Purpose

Constitutive lentiviral expression of YAP1 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP204-Lenti-TRE3G-GFP-WPRE-Pa

Plasmid

#164788

Purpose

pLenti vector backbone designed to express GFP from a TRE3G promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

Lentiviral

Promoter

1.3αCaMKII

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2370 - intron GG3 - 250bp

Plasmid

#159879

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG3 - 250bp

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2259 - Intron GG2 - 900 bp

Plasmid

#159881

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - 900 bp

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pG3B-U3Ub

Plasmid

#134750

Purpose

pGreen3 CRISPR/Cas9

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Promoter

gRNA- OsU3, zCas9-Ubi1

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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tubulin-ReNL_pcDNA3

Plasmid

#89530

Purpose

Red color variant of bright luminescent protein, fused to b-tubulin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Tags

b-tubulin

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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Cox8x2-YeNL_pcDNA3

Plasmid

#89517

Purpose

Yellow color variant of bright luminescent protein, targeted to the Mitochondria

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Tags

CoxVIII X2

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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Cox8x2-ReNL_pcDNA3

Plasmid

#89533

Purpose

Red color variant of bright luminescent protein, targeted to the Mitochondria

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Tags

CoxVIII X2

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pTU2-a-RFP

Plasmid

#72958

Purpose

Level 2 Destination vector - Position A - Assembly of 2 TU's from Level 1-AB

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 2 - a site

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
MC177VSG221RNAi

Plasmid

#59730

Purpose

For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

Depositor

Gloria Rudenko

Article

Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

Insert

VSG221 RNAi fragment

Use

RNAi

Promoter

opposing T7 promoters

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-Ub(L40)

Plasmid

#52892

Purpose

Aedes aegypti ubiquitin promoter driving firefly luciferase

Depositor

Zach Adelman

Article

Anderson et al Insect Mol Biol. 2010 Aug;19(4):441-9. d

Insert

ubiquitin promoter

Use

Luciferase

Promoter

Ae aegypti ubiquitin

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

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p3.5VPIII.EGFP.IGR2.1

Plasmid

#40865

Depositor

Harold Gainer

Article

Fields et al J Neurosci. 2003 Aug 27;23(21):7801-9.

Insert

Mouse vasopressin gene (Avp Mouse)

Expression

Bacterial

Promoter

AVP

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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