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gRNA_AAVS1-T2

Plasmid

#41818

Purpose

Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_AAVS1-T2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST-cuOx144_Bxb1attB_loxP-PGKpuro-loxP

Plasmid

#119903

Purpose

Bxb1 integration plasmid for cuO array with Puro selection cassette

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

cuOx144 array

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pGD283

Plasmid

#129733

Purpose

Destination plasmid containing the 35S:XVE estradiol inducible driver and the A-G overhangs for GreenGate cloning of an expression casette for estradiole-inducible expression in plants.

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE)

Plasmid

#214875

Purpose

Empty lentiviral backbone for guide array cloning

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCXLE-mCherry

Plasmid

#193293

Purpose

episomal vector expressing mCherry fluorescent protein

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-s ONE-GO

Plasmid

#189731

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-s ONE-GO
GNAS (GNAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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NylCK_pET28a

Plasmid

#215422

Purpose

Expression construct for NylCK gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCK

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pXPR_120

Plasmid

#96917

Purpose

for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR

Depositor

David Root, John Doench

Article

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Insert

dCas9

Use

Lentiviral

Tags

VPR

Expression

Mammalian

Mutation

D10A, N863A

Promoter

EF1a

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCX-HO1-2A-EGFP

Plasmid

#74672

Purpose

Mammalian expression of HO1 and EGFP

Depositor

Roberto Giovannoni

Article

Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

Insert

HO1 (HMOX1 Human)

Use

Tags

2A-EGFP

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pPY22-mNeonGreen-Hyg

Plasmid

#137082

Purpose

Codon-optimized mNeonGreen for expression in Arabidopsis thaliana and Physcomitrella patens

Depositor

Gohta Goshima

Article

Yi et al Curr Biol. 2020 Jul 20;30(14):2860-2868.

Insert

mNeonGreen-Flag

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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