19,660 results

1. pF146 pSOD1WTAcGFP1

   Plasmid

   #26407

   Depositor

   Elizabeth Fisher

   Article

   Stevens et al PLoS One. 2010 . 5(3):e9541.

   Insert

   SOD1 (SOD1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Nov. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLVX-Tight-Puro_MYRF

   Plasmid

   #64843

   Purpose

   A lentiviral vector for the expression of mouse MYRF under doxycyline control.

   Depositor

   Paul Tesar

   Article

   Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

   Insert

   MYRF (Myrf Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FU - dio YFP - W

   Plasmid

   #73858

   Purpose

   For expressing YFP in mammalian cells upon Cre co-expression. Can be made into Lentivirus.

   Depositor

   Elly Nedivi

   Article

   Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

   Insert

   eYFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONRp5-p2 GFP-NShroom3-iLID

   Plasmid

   #170976

   Purpose

   N-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.

   Depositor

   Miki Ebisuya

   Article

   Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

   Insert

   NShroom3 (Shroom3 Mouse)
   

   Use

   Gateway cloning

   Tags

   eGFP and iLID

   Mutation

   From isoform 2, deleted amino acids 1389 - 1805,…

   Available Since

   July 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONRp5-p2 SspB-mCherry-CShroom3

   Plasmid

   #170977

   Purpose

   C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.

   Depositor

   Miki Ebisuya

   Article

   Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

   Insert

   CShroom3 (Shroom3 Mouse)
   

   Use

   Gateway cloning

   Tags

   SspB and mCherry

   Mutation

   Isoform X2, deleted aminoacids 1 - 1388

   Available Since

   July 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT7-NSP5SA11

   Plasmid

   #89172

   Purpose

   Express Rotavirus SA11 NSP5 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 NSP5
   

   Expression

   Mammalian

   Promoter

   T7 promotor

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1 Flag Runx1 FL (P#1790)

   Plasmid

   #14585

   Depositor

   Matija Peterlin

   Article

   Jiang et al Mol Cell Biol. 2005 Dec . 25(24):10675-8

   Insert

   Runx1 (Runx1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p-dCas9-SSAP-MS2-BB_BbsI

   Plasmid

   #183826

   Purpose

   pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

   Depositor

   Le Cong

   Article

   Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

   Insert

   U6-guideRNA-dCas9-T2A-EBFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CBH

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A

   Plasmid

   #154773

   Purpose

   plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y125A in G1PylS, hybrid PylT with A41AA and C55A …

   Promoter

   EF1

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEFIRES-P-mTagBFP-KDEL

    Plasmid

    #87163

    Purpose

    Fluorescent marker of endoplasmic reticulum (ER)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    mTagBFP-KDEL
    

    Tags

    Bip signal peptide (Homo sapiens) and mTagBFP-KDE…

    Expression

    Mammalian

    Promoter

    EF-1 alpha

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT7-NSP2SA11

    Plasmid

    #89169

    Purpose

    Express Rotavirus SA11 NSP2 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 NSP2
    

    Expression

    Mammalian

    Promoter

    T7 promotor

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB

    Plasmid

    #122668

    Purpose

    C terminal domain of hnRNPA1 (186-320) tagged with SspB and mCherry.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    HNRNPA1 (HNRNPA1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-SspB

    Expression

    Mammalian

    Mutation

    Contains only amino acids 186-230

    Promoter

    SFFV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCW57.1 Lysosomal-METRIQ

    Plasmid

    #135401

    Purpose

    Expresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activity

    Depositor

    Eisuke Itakura

    Article

    Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

    Insert

    DNAse II alpha (DNASE2 Human)
    

    Use

    Lentiviral

    Tags

    T2A, mCherry, and sfGFP

    Expression

    Mammalian

    Mutation

    Single-nucleotide polymorphism C432T

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mscv2.2

    Plasmid

    #60206

    Purpose

    Mammalian cell expression plasmid; drives constitutive, low-level expression from retroviral LTR; retroviral vector

    Depositor

    Russell Vance

    Article

    Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. G6PD/pRK5

    Plasmid

    #41521

    Depositor

    Xiaolu Yang

    Article

    Jiang et al Nat Cell Biol. 2011 Mar;13(3):310-6. Epu

    Insert

    glucose-6-phosphate dehydrogenase (G6PD Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EGFP-Bak

    Plasmid

    #32564

    Depositor

    Richard Youle

    Article

    Nechushtan et al J Cell Biol. 2001 Jun 11. 153(6):1265-76

    Insert

    human Bak (BAK1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHR-BRD4ΔN-mCherry-sspB

    Plasmid

    #121968

    Purpose

    Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-sspB

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-461

    Promoter

    SFFV

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCXLE-gw

    Plasmid

    #37626

    Purpose

    Non-integrating (episomal) expression vector with Gateway cassette

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDule-tfmF A65V S158A

    Plasmid

    #85484

    Purpose

    Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

    Insert

    tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32V F108W Q109M I159M with 65Val and 158Ala

    Promoter

    lpp (constitutive)

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETM6

    Plasmid

    #49795

    Purpose

    ePathBrick vector for pathway engineering in E. coli.

    Depositor

    Mattheos Koffas

    Article

    Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

    Type

    Empty backbone

    Tags

    S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge