165,567 results

1. TFORF2554

   Plasmid

   #144129

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   AR (AR Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWPI

   Plasmid

   #12254

   Purpose

   2nd gen bicistronic lentiviral vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells.

   Depositor

   Didier Trono

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox and Lentiviral

   Tags

   EMCV IRES-EGFP cassette

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgCtr- LentiCRISPRv2

   Plasmid

   #107402

   Purpose

   Lentiviral expression of Cas9 and a control gRNA

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   control gRNA
   

   Use

   Lentiviral

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmGFP-P2A-K(AAA)20-P2A-RFP

   Plasmid

   #105688

   Purpose

   Double fluorescent stalling reporter K(AAA)20

   Depositor

   Ramanujan Hegde

   Article

   Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

   Inserts

   P2A-3xFLAG-VHPbeta
   K(AAA)20
   P2A-mCherry
   

   Expression

   Mammalian

   Promoter

   CMV (from vector)

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. MIB2-FLAG

   Plasmid

   #37117

   Depositor

   Martin Dorf

   Article

   Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

   Insert

   MIB2, mindbomb E3 ubiquitin protein ligase 2 (MIB2 Human)
   

   Tags

   3x FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

6. WT FL USP7

   Plasmid

   #227214

   Purpose

   Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Tags

   6His

   Expression

   Bacterial

   Promoter

   T7-promoter

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Paxillin-pEGFP

   Plasmid

   #15233

   Depositor

   Rick Horwitz

   Article

   Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

   Insert

   paxillin (PXN Chicken)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS134

   Plasmid

   #211505

   Purpose

   AAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

   Depositor

   Randall Platt

   Article

   Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

   Insert

   Venus
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Expression

   Mammalian

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-HaloTag-KanMX6

   Plasmid

   #87029

   Purpose

   Tag S. pombe genes in C terminal with HaloTag

   Depositor

   Julien Berro

   Insert

   HaloTag
   

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWALIUM-intron 3x UAS attB

    Plasmid

    #206360

    Purpose

    includes two standard UAS cassettes and one UAS cassette designed to co-express shRNAs/shRNA clusters and prodein coding sequences

    Depositor

    Erdem Bangi

    Article

    Teague et al PLoS Genet. 2023 Jun 2;19(6):e1010792. d

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pscAAV-Sox2-mStr

    Plasmid

    #135617

    Purpose

    Donor vector for genomic targeting of a P2A-mStrawberry cassette to the 3' end of the mouse Sox2 locus

    Depositor

    Lin He

    Article

    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

    Insert

    P2A-mStrawberry flanked by Sox2 homology arms (Sox2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    P2A-mStrawberry

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDualIN-SMN2

    Plasmid

    #214430

    Purpose

    Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    SMN2 EXON 7 (SMN2 Human)
    

    Expression

    Mammalian

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEHlyA5

    Plasmid

    #168298

    Purpose

    Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

    Depositor

    Luis Ángel Fernández

    Article

    Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

    Insert

    N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
    

    Tags

    E-tag, HA-tag, His-tag, Prescission site, and VHH…

    Expression

    Bacterial

    Promoter

    LacI-Plac

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST40-2XHA-wtSrc

    Plasmid

    #140294

    Purpose

    Expresses HA-tagged wild-type human Src in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src wildtype (SRC Human)
    

    Tags

    2X HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Mariner2_AG

    Plasmid

    #217135

    Purpose

    Mammalian expression vector for expression of Mariner2_AG transposase (CMV promoter)

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    Mariner2_AG transposase
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-Merlin

    Plasmid

    #84293

    Purpose

    Expression of wt Merlin

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Merlin (NF2) (NF2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCVpuro-Cre

    Plasmid

    #34564

    Purpose

    Retroviral expression of Cre

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

    Insert

    Cre
    

    Use

    Cre/Lox and Retroviral

    Expression

    Mammalian

    Promoter

    5'LTR

    Available Since

    Jan. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-M13-TEV-C-P2A-TdTomato

    Plasmid

    #92391

    Purpose

    produces AAV expressing Cal-light M13-TevC component and TdTomato

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    M13-TEV-C-P2A-TdTomato
    

    Use

    AAV and Synthetic Biology

    Tags

    TdTomato

    Expression

    Mammalian

    Promoter

    human synapsin

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-Rab7a-7

    Plasmid

    #55127

    Purpose

    Localization: Ras GTPase, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Rab7a (RAB7A Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    N117S in NM_004637.5

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C3-Sec5

    Plasmid

    #53756

    Purpose

    pEGFP-C3 plasmid that expresses Sec5

    Depositor

    Channing Der

    Article

    Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

    Insert

    Sec5 (EXOC2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only