We narrowed to 8,430 results for: APP

1. pcDNA5-FRT/TO-3*Flag-APEX2 N-term SRSF1

   Plasmid

   #187575

   Purpose

   Express FLAG epitope and APEX2-tagged SRSF1 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   SRSF1 (SRSF1 Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONR223 SARS-CoV-2 ORF9B

   Plasmid

   #141280

   Purpose

   Gateway-compatible Entry vector, with insert of ORF9B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF9B (N SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   April 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pDONR223 SARS-CoV-2 ORF7B

   Plasmid

   #141277

   Purpose

   Gateway-compatible Entry vector, with insert of ORF7B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   April 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pDONR223 SARS-CoV-2 NSP9

   Plasmid

   #141263

   Purpose

   Gateway-compatible Entry vector, with insert of NSP9 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP9 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   April 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pDEST-N2H-N1

   Plasmid

   #125547

   Purpose

   Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment1

   Depositor

   Marc Vidal

   Article

   Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

   Type

   Empty backbone

   Use

   In vitro expression

   Tags

   NanoLuc fragment1 attached to n-terminus of Gatew…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   synthetic promoter containing yeast, mammalian, a…

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDEST-N2H-C1

   Plasmid

   #125549

   Purpose

   Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment1

   Depositor

   Marc Vidal

   Article

   Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

   Type

   Empty backbone

   Use

   In vitro expression

   Tags

   NanoLuc fragment1 attached to c-terminus of Gatew…

   Expression

   Mammalian and Yeast

   Mutation

   Promoter

   synthetic promoter containing yeast, mammalian, a…

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDEST-N2H-C2

   Plasmid

   #125559

   Purpose

   Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment2

   Depositor

   Marc Vidal

   Article

   Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

   Type

   Empty backbone

   Use

   In vitro expression

   Tags

   NanoLuc fragment2 attached to c-terminus of Gatew…

   Expression

   Mammalian and Yeast

   Mutation

   Promoter

   synthetic promoter containing yeast, mammalian, a…

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDEST-N2H-N2

   Plasmid

   #125548

   Purpose

   Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2

   Depositor

   Marc Vidal

   Article

   Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

   Type

   Empty backbone

   Use

   In vitro expression

   Tags

   NanoLuc fragment2 attached to n-terminus of Gatew…

   Expression

   Mammalian and Yeast

   Mutation

   Promoter

   synthetic promoter containing yeast, mammalian, a…

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-CIB1-mRFP1-MP

   Plasmid

   #58367

   Purpose

   For use in light-induced protein inactivation through the interaction with CRY2

   Depositor

   Won Do Heo

   Article

   Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

   Inserts

   CIB1[1-147] (CIB1 Mustard Weed)
   Camk2a[315-478] (Camk2a Rat)
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKK-RNAi-nucCHERRYmiR-EGFP-TEV

    Plasmid

    #105810

    Purpose

    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal EGFP.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    RNAi; Flp-in competent

    Tags

    EGFP-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKK-RNAi-nucCHERRYmiR-FLAG-TEV

    Plasmid

    #105809

    Purpose

    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal FLAG.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    RNAi; Flp-in competent

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5-FRT/TO-3*Flag-APEX2 N-term COILIN

    Plasmid

    #187581

    Purpose

    Express FLAG epitope and APEX2-tagged COILIN fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    COILIN (COIL Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5

    Plasmid

    #97044

    Purpose

    Expresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Ligand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet on

    Available since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

    Plasmid

    #122085

    Purpose

    Express M.EcoGII fused to CENPC with destabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII-V5-CENPC (CENPC E.coli, Human)
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of CENPC

    Expression

    Bacterial and Mammalian

    Mutation

    L341F, R930L (see comments below)

    Promoter

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3

    Plasmid

    #105802

    Purpose

    Concomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-mRuby3; ORF2: TEV-mClover3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBabe-Puro-Flag-IKBKE

    Plasmid

    #15292

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKBKE (IKBKE Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pBFC0625

    Plasmid

    #186691

    Purpose

    VcDART under control of Lac promoter, Vc_lacZ_α_1 gRNA, GmR+sfGFP cargo

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_lacZ_α_1 Spacer crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBabe-Neo-Flag-IKBKE

    Plasmid

    #15265

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKBKE (IKBKE Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pBFC0687

    Plasmid

    #186692

    Purpose

    ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_2xBsaI_NT gRNA, GmR+sfGFP cargo

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsB
    tnsC
    tniQ
    Cas12k
    Sh_2xBsaI_NT (Guide Stuffer) gRNA
    Tn7 transposon
    GmR+sfGFP ShDART Cargo
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBabe-Puro-cRAF-W22

    Plasmid

    #15270

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    CRAF (RAF1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    C-terminal deletion

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only