19,660 results

1. piRFP

   Plasmid

   #31857

   Purpose

   a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

   Depositor

   Vladislav Verkhusha

   Article

   Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

   Has Service

   Cloning Grade DNA

   Insert

   iRFP
   

   Expression

   Mammalian

   Mutation

   T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

   Promoter

   CMV

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-HRP-C

   Plasmid

   #40163

   Depositor

   Paul Ortiz de Montellano

   Article

   Hartmann et al Arch Biochem Biophys. 1992 Aug 15;297(1)

   Insert

   Horseradish peroxidase
   

   Use

   Coli cloning vector

   Tags

   Six histidine

   Available Since

   Nov. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pet42c(+)-GST-USP5

   Plasmid

   #61750

   Purpose

   express N-terminally GST tagged human USP5

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   USP5 (USP5 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCRISPRyl

   Plasmid

   #70007

   Purpose

   CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2015 Dec 29.

   Inserts

   Codon optimized Cas9 from S. pyogenes
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

   Plasmid

   #171379

   Purpose

   Expression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

   Depositor

   Hui Yang

   Article

   Xu et al Nat Methods. 2021 May;18(5):499-506. doi

   Insert

   humanized Cas13X.1
   

   Expression

   Mammalian

   Promoter

   CMV, U6

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-Brainbow-2.1 R

   Plasmid

   #18723

   Depositor

   Joshua Sanes

   Article

   Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

   Insert

   hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   Aug. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.hSynap.iAChSnFR

   Plasmid

   #137950

   Purpose

   Expresses iAChSnFR under hSynapsin promoter

   Depositor

   Loren Looger

   Article

   Borden et al bioRxiv 2020

   Has Service

   AAV1

   Insert

   iAChSnFR
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSynanpsin

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. plRL2

   Plasmid

   #163631

   Purpose

   Optimized M. tuberculosis CRISPRi plasmid; L5 integrating.

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   Tet-On

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3 Flag p38 alpha

   Plasmid

   #20351

   Depositor

   Roger Davis

   Article

   Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

   Insert

   pDCNA3 Flag p38 alpha (Mapk14 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7-VP7SA11

    Plasmid

    #89167

    Purpose

    Express Rotavirus SA11 VP7 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP7
    

    Tags

    *see below

    Expression

    Mammalian

    Promoter

    T7 promotor

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-F-AF9

    Plasmid

    #49428

    Purpose

    Mammalian expression of Flag-tagged human AF9

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    AF-9 (MLLT3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. Human Parkin (1-465)

    Plasmid

    #110758

    Purpose

    Bacterial expression of human Parkin

    Depositor

    David Komander

    Article

    Wauer et al EMBO J. 2013 Jul 31;32(15):2099-112. doi

    Insert

    PRKN (PRKN Human)
    

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. ZJOM65

    Plasmid

    #133654

    Purpose

    Integration of VPH1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-VPH1-mCherry (VPH1 Budding Yeast)
    

    Tags

    mCherry

    Expression

    Yeast

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. ins:CFP-NTR

    Plasmid

    #66593

    Purpose

    Specific and conditional cell ablation of beta cells in the zebrafish pancreas

    Depositor

    Didier Stainier

    Article

    Curado et al Dev Dyn. 2007 Apr . 236(4):1025-35.

    Insert

    Insulin promotor driving CFP-nitroreductase
    

    Use

    Zebrafish expression

    Tags

    CFP fused with N-terminus of nitroreductase

    Promoter

    Zebrafish Insulin promoter (approximately 1.2kb)

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pET His6 LIC cloning vector (2Bc-T)

    Plasmid

    #37236

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. YE1-BE4max-NG

    Plasmid

    #138159

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R126E; within Cas9 D10A + …

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. RpHLuorin2

    Plasmid

    #180227

    Purpose

    for bacterial expression of recombinant ratiometric pHluorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2
    

    Tags

    His-tag followed by thrombin cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-C1-CAMKIIalpha

    Plasmid

    #21226

    Depositor

    Tobias Meyer

    Article

    Shen et al Neuron. 1998 Sep . 21(3):593-606.

    Insert

    CAMKIIalpha (Camk2a Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Lab plasmid KS 0001.

    Available Since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. CBIG-RORb

    Plasmid

    #48709

    Purpose

    bicistronic expression of RORb and EGFP

    Depositor

    Jeffrey Macklis

    Insert

    RORb (Rorb Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV/beta-actin

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. tsPurple chromoprotein

    Plasmid

    #117848

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses tsPurple chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, tsPurple
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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