We narrowed to 9,972 results for: crispr plasmids
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TC9pAB
Plasmid#71310Purposeused as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsIDepositorInsertCas9
UseIn vitro transcriptionTagsNLSPromoterT7Available SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWN_U6-TRAC-miniGag
Plasmid#228960PurposeminiEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)DepositorInsertminiGag
UseCRISPRExpressionMammalianAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBLU-VIPR
Plasmid#220498PurposePlasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).DepositorTypeEmpty backboneUseCRISPR; TransposonExpressionMammalianAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1180 U6-reci Gag-pol v2
Plasmid#201915PurposeCas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)DepositorInsertGag-pol
ExpressionMammalianPromoterCAGAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
dSaCas9-VPR
Plasmid#205251PurposeIn vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495DepositorInsertdSaCas9-VPR
UseCRISPRAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1
Plasmid#89693PurposeLuciferase reporter plasmid targeted at csr-1 locusDepositorInsertgsy-1 promoter, luciferase
UseYeast episomal vectorTagsluciferasePromotergsy-1Available SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
dSpCas9-VPR
Plasmid#205247PurposeIn vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.DepositorInsertdSpCas9-VPR
UseCRISPRAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAT15516_BEAR-GFP-2in1
Plasmid#174082PurposeBEAR-GFP plasmid with a gRNA that targets its own plasmidDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRExpressionMammalianMutationdisrupted 5' splice sitePromoterCMVAvailable SinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW-tTRE-scFv-SunTag
Plasmid#193138PurposeExpresses scFv intracellular antibody component of the SunTag system under Dox regulationDepositorInsertscFv-SunTag
UseLentiviralExpressionMammalianPromotertight TRE promoterAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
KRAB-dSpCas9
Plasmid#205248PurposeIn vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.DepositorInsertKRAB-dSpCas9
UseCRISPRAvailable SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP-Cetn1
Plasmid#50717PurposeCetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.DepositorAvailable SinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEditA
Plasmid#207531PurposePlasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpRDepositorInsertPlasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA
UseCRISPR; Pseudomonas vectorAvailable SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-BLU-VIPR
Plasmid#220497PurposeRetroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).DepositorTypeEmpty backboneUseCRISPR and RetroviralExpressionMammalianAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAN056
Plasmid#220052PurposeReporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (positive control).DepositorInsertfLuc-CFTR (exons 22-27) (CFTR Human)
ExpressionMammalianAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDM097
Plasmid#216819PurposeAspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for N-terminal or internal tagging.DepositorInsertmStayGold
TagsFLAG-(SGGS)x2-XTEN16-(GGGGS)x3 and c4-(GGGGS)x2-X…ExpressionBacterialMutationAspergillus nidulans codon-adjustedAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only
