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pETM33_Orf10

Plasmid

#156486

Purpose

Bacterial expression of Sars-CoV2 Orf10 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Orf10 (ORF10 Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-HA-Mettl3

Plasmid

#196262

Purpose

In vitro transcription/translation of HA-tagged wild-type Mettl3

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

Mettl3 (Mettl3 Mouse)

Tags

3xHA

Expression

Mammalian

Promoter

CMV IE94

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(92Q)-S776D

Plasmid

#21757

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 92 Q(Glutamines) and…

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
GFP-ataxin-3 N-t

Plasmid

#89980

Purpose

Expresses the GFP-tagged N-terminus (Josephin domain) of ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

construct contains aa 1-182; deleted aa 183-361

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3/hArf5(WT)-EGFP

Plasmid

#79534

Purpose

Expresses C-terminally EGFP-tagged Arf5(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf5 (ARF5 Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pQCXIB Flag MFF WT

Plasmid

#74384

Purpose

Retroviral expression vector containing Flag tagged WT MFF isoform 5

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only
p3xFLAG-CMV TIP60 212-364

Plasmid

#78795

Purpose

To overexpress TIP60 212-364 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

TIP60 (KAT5 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

212-364, G214A

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
GFP-ataxin-3 C-t

Plasmid

#89981

Purpose

Expresses the GFP-tagged C-terminus of ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-182

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MFF iso5 S172A

Plasmid

#74382

Purpose

Flag tagged human S172A MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser 172 Ala (numbering based on MFF isoform 1)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MFF iso5 S155,172A

Plasmid

#74383

Purpose

Flag tagged human S155,172A MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Tags

Flag

Expression

Mammalian

Mutation

See 155 Ala, Ser 172 Ala (numbering based on MFF …

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
p426_Cas9_gRNA-R-ARS607c

Plasmid

#87409

Purpose

p426_Cas9_gRNA-ARS607c without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS607c sequence CTATTTTTGCTTTCTGCACA in yeast chromosome 6.

Depositor

Aindrila Mukhopadhyay

Article

Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

Insert

Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS607c

Use

CRISPR

Promoter

ADH1, pTyrosine

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pRRLsin.PPT.CMV‐GFP‐Eps8 WT

Plasmid

#74922

Purpose

Lentiviral plasmid expressing Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Menna et al PLoS Biol. 2009 Jun 30;7(6):e1000138. do

Insert

Eps8 (Eps8 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only
pET41s-GST-ATF6a-AD

Plasmid

#64774

Purpose

bacterial expression of GST-tagged ATF6 (1-150)

Depositor

Joan Conaway, Ronald Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

ATF6a-AD (1-150) (ATF6 Human)

Tags

GST

Expression

Mammalian

Mutation

C-term deletion

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
CmCitrine Lck w/ NosUTRs

Plasmid

#66785

Purpose

expression of fluorescent membranes in sea urchin primordial germ cells

Depositor

Amro Hamdoun

Article

Campanale et al Dev Dyn. 2014 Jul;243(7):917-27. doi: 10

Insert

Lck (LCK Human)

Use

Sea urchin, zebrafish, xenopus

Tags

3' UTR from sea urchin Nanos, 5' UTR fr…

Mutation

Tandem repeat of LCK the N-terminal membrane targ…

Promoter

T7

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 FLAG mTOR 1968-2549 S2035T

Plasmid

#23000

Depositor

Jing Huang

Article

Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

Insert

mTOR (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

S2035T aa 1968-2549 only

Available Since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT

Plasmid

#89890

Purpose

BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.

Depositor

Tatjana Sauka-Spengler

Article

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

HA-BirA-2A-citrine

Use

Unspecified

Tags

Citrine

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV TIP60 390-513

Plasmid

#78797

Purpose

To overexpress TIP60 390-513 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

TIP60 (KAT5 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

aa390-513

Promoter

CMV

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pETM33_Nsp3c_SUD-N

Plasmid

#156469

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-N protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-N (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3c_SUD-M

Plasmid

#156470

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-M protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-M (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET45b-tsr(B/X) (6X-His-Dm-Cofilin)

Plasmid

#128278

Purpose

Expresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell culture

Depositor

Anna Sokac

Article

Figard et al Cell Rep. 2019 Mar 26;26(13):3493-3501.e

Insert

twinstar (tsr) (tsr Fly)

Tags

6X Histidine tag

Expression

Bacterial

Mutation

Met1 changed to Pro1; Cys77 silent basepair mutat…

Promoter

T7

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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