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pGWB423

Plasmid

#74817

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pSicoR human Drosha2

Plasmid

#14767

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Drosha shRNA (DROSHA Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pCAG--uMASS_LF

Plasmid

#209763

Purpose

Expression of uMASS_LF (loss-of-function) in mammalian cells under CAG promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Expression

Mammalian

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-uMASS_1

Plasmid

#209762

Purpose

Expression of uMASS_1 in mammalian cells under CAG promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_1

Expression

Mammalian

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pSuper mouse DGCR8-1

Plasmid

#14772

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

DGCR8 shRNA (Dgcr8 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
HB501: pMVP (L1-L4) RIP

Plasmid

#121688

Purpose

pMVP L1-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 3-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Rat Ins1 promoter (RIP; β-cell specific)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMAX deltaN-ERG

Plasmid

#29447

Depositor

Valeri Vasioukhin

Article

Klezovitch et al Proc Natl Acad Sci U S A. 2008 Feb 12. 1

Insert

ERG (ERG Human)

Expression

Mammalian

Mutation

Deletion of first 33 aa of ERG

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
KG#203

Plasmid

#110880

Purpose

Expresses the C. elegans pde-4 (isoform d) cDNA pan-neuronally

Depositor

Kenneth Miller

Article

Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1

Inserts

rab-3 promoter
pde-4d (isoform d) cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Consensus

Plasmid

#176664

Purpose

Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB450

Plasmid

#74832

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBS mouse c-Myc

Plasmid

#14972

Depositor

Joan Massague

Article

Siegel et al J Biol Chem. 2003 Sep 12. 278(37):35444-

Insert

c-Myc (Myc Mouse)

Expression

Bacterial

Mutation

Nucleotides 2578-3294

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pCI pHluorin Syt11

Plasmid

#184512

Purpose

Mammalian expression of Synaptotagmin 11 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 11 (Syt11 Mouse)

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Myo10-∆PH

Plasmid

#139781

Purpose

Detecting Myo10-∆PH by fluorescence microscopy

Depositor

Staffan Stromblad

Article

Plantard et al J Cell Sci. 2010 Oct 15;123(Pt 20):3525-

Insert

Myosin X (MYO10 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Deletion of 3PH domains

Promoter

CMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGK:HygroR-CMV:mTagBFP2-A_UTR

Plasmid

#68479

Purpose

Lentivirus expressing mTagBFP2 with UTR sensor cassette and hygromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Hygromycin Resistance
mTagBFP2-A_UTR

Use

Lentiviral

Expression

Mammalian

Mutation

shRNA sensor cassette "A" added to 3&#x…

Promoter

CMV and pGK

Available Since

Nov. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FMR1iso1-G266E

Plasmid

#225468

Purpose

Express mEGFP-fusion protein of FMR1 isoform 1 (G266E)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FMR1 isoform 1 (FMR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

G266E

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FMR1iso1-I304N

Plasmid

#225463

Purpose

Express mEGFP-fusion protein of FMR1 isoform 1 (I304N)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FMR1 isoform 1 (FMR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

I304N

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Poly(A)

Plasmid

#65524

Purpose

Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pMM2-hPMCA2w/b

Plasmid

#47583

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA2w/b (ATP2B2 Human)

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Aaeg_774

Plasmid

#176659

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pEXL flag Smad3

Plasmid

#10920

Depositor

Bob Weinberg

Article

Liu et al Proc Natl Acad Sci U S A. 1997 Sep 30. 9

Insert

Smad3 (SMAD3 Human)

Tags

flag

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only