We narrowed to 9,085 results for: mel

1. pINDUCER20 MEF2C

   Plasmid

   #89717

   Purpose

   Lentiviral gene expression vector for doxycycline inducible MEF2C expression

   Depositor

   Alex Kentsis

   Article

   Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

   Insert

   MEF2C (MEF2C Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. P3-Lenti-dCas9-Tet1-GFP

   Plasmid

   #190729

   Purpose

   Lentiviral construct to express spdCas9-Tet1-GFP.

   Depositor

   Maja Jagodic

   Article

   Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

   Insert

   dCas9-tet1
   

   Use

   CRISPR

   Tags

   SV40 NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   Changed Pro434 codon from CCG to CCC and Gly468 f…

   Promoter

   UbC

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3-Flag-SPRTN-wt

   Plasmid

   #110214

   Purpose

   Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

   Depositor

   Ivana Marinovic-Terzic, Kristijan Ramadan

   Article

   Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

   Insert

   SPRTN (SPRTN Human)
   

   Expression

   Mammalian

   Mutation

   P296L (see depositor comments below)

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-human OCT4 PE-SV40-Luc

   Plasmid

   #52415

   Purpose

   Luciferase reporter plasmid for PE OCT4 enhancer activity

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Insert

   Proximal element of hOCT4 promoter (POU5F1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-human OCT4 DE-SV40-Luc

   Plasmid

   #52414

   Purpose

   Luciferase reporter plasmid for DE OCT4 enhancer activity

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Insert

   Distal element of human OCT4 promoter (POU5F1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPaint-mNeon-F-mgp100-PuroR [M1G]

   Plasmid

   #171802

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-FLAG-mgp100-T2A-PuroR
   

   Use

   Gene tagging

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-IRES-Tomato MEF2C

   Plasmid

   #89715

   Purpose

   Retroviral gene expression vector for MEF2C expression

   Depositor

   Alex Kentsis

   Article

   Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

   Insert

   MEF2C (MEF2C Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   June 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

   Plasmid

   #52419

   Purpose

   Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Insert

   ERAS (ERAS Human)
   

   Promoter

   CAGGS

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

   Plasmid

   #216733

   Purpose

   Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase and sgCTG
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   U6 and CAG

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX551-miniCMV-SpCas9D10A nickase

    Plasmid

    #216735

    Purpose

    Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    T7 and mini-CMV

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pINDUCER20 MEF2C S222A

    Plasmid

    #89718

    Purpose

    Lentiviral gene expression vector for doxycycline inducible MEF2C-S222A expression

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MEF2C (MEF2C Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Changed serine 222 to alanine

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

    Plasmid

    #171801

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-hgp100-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. TMEM230-M4/X184PG-IRES(isoform 2)

    Plasmid

    #99568

    Purpose

    mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

    Depositor

    Han-Xiang Deng

    Article

    Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

    Insert

    TMEM230 (TMEM230 Human)
    

    Expression

    Mammalian

    Mutation

    M4/X184PG

    Promoter

    CMV

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. TMEM230-M3/Y92C-IRES(isoform 2)

    Plasmid

    #99567

    Purpose

    mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

    Depositor

    Han-Xiang Deng

    Article

    Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

    Insert

    TMEM230 (TMEM230 Human)
    

    Expression

    Mammalian

    Mutation

    M3/Y92C

    Promoter

    CMV

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. TMEM230-M2/X184W-IRES(isoform 2)

    Plasmid

    #99566

    Purpose

    mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

    Depositor

    Han-Xiang Deng

    Article

    Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

    Insert

    TMEM230 (TMEM230 Human)
    

    Expression

    Mammalian

    Mutation

    M2/X184W

    Promoter

    CMV

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. TMEM230-M1/R141L-IRES(isoform 2)

    Plasmid

    #99565

    Purpose

    mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

    Depositor

    Han-Xiang Deng

    Article

    Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

    Insert

    TMEM230 (TMEM230 Human)
    

    Expression

    Mammalian

    Mutation

    M1/R141L

    Promoter

    CMV

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV-IRES-Tomato MEF2C S222A

    Plasmid

    #89716

    Purpose

    Retroviral gene expression vector for MEF2C-S222A expression

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MEF2C (MEF2C Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPaint-mScarlet-F-Ova-PuroR [M1G]

    Plasmid

    #171813

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-F-Ova-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-IRES_Tomato MEF2C S222D

    Plasmid

    #107231

    Purpose

    hMEF2C phosphomimetic obtained from Quikchange Lightning Mutagenesis

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MEF2C (MEF2C Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    S222D

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. TMEM230-M2/X184W-IRES(isoform 1)

    Plasmid

    #99560

    Purpose

    mammalian expression of mutant TMEM230 (isoform 1) and ZsGreen

    Depositor

    Han-Xiang Deng

    Article

    Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

    Insert

    TMEM230 (TMEM230 Human)
    

    Expression

    Mammalian

    Mutation

    M2/X184W

    Promoter

    CMV

    Available Since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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