We narrowed to 9,939 results for: CAG

Showing: 6961 - 6980 of 9939 results

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lenti-GBA-N370S-tmpknot-epegRNA

Plasmid

#214091

Purpose

Lentiviral vector expressing epegRNA to induce GBA N370S mutation

Depositor

Ting Zhou

Publication

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

GBA N370S epegRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry

Plasmid

#210504

Purpose

expresses NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry component in mammalian cells

Depositor

Dongmin Lee

Publication

Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry

Expression

Mammalian

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry-SPASTIN, F124D

Plasmid

#180648

Purpose

Lentiviral expression vector for SPASTIN. Has F124D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-44.

Depositor

Wesley Sundquist

Publication

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Starts at M87, F124D mutation, has siRNA resistan…

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mPHB2-1

Plasmid

#198483

Purpose

lentiviral stable expression of mPHB2 gRNA 1

Depositor

Suzanne Pfeffer

Publication

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mPhb2 (Phb2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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sgCRY2

Plasmid

#197457

Purpose

Expresses sgRNA(CRY2 gene) in mammalian cells

Depositor

Han Kyoung Choe

Publication

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCRY2-1-hU6-sgCRY2-2 (Cry2 Mouse)

Use

AAV

Tags

mCherry (K14E, N210D, E249G)

Promoter

U6, hSyn

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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ND4-FusXTBE-Left TALE

Plasmid

#196496

Purpose

Expresses left-side TALE-DddAtox Cterm half ND4-FusXTBE in mammalian cells

Depositor

Stephen Ekker

Publication

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-ND4 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR

Expression

Mammalian

Available since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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ND4-FusXTBE-Right TALE

Plasmid

#196497

Purpose

Expresses Right-side TALE-DddAtox Nterm half ND4-FusXTBE in mammalian cells

Depositor

Stephen Ekker

Publication

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-ND4 right TALE-G1397 DddA-C-UGI-ATP5B 3'UTR

Expression

Mammalian

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-mNeurog2 gRNA_1-MS2-Puro

Plasmid

#192680

Purpose

Lentiviral expression of sgRNA targeting mIL1RN promoter to activate mouse Neurog2 transcription

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Mouse Neurog2 activating gRNA #1 (Neurog2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUbC-EBPF2-gRNA-TGFB2

Plasmid

#185556

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting TGFB2

Depositor

Andrew Wilson

Publication

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

TGFB2 gRNAs

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pYJ34-PB-CRISPR-BANCR-E1-gRNA

Plasmid

#131077

Purpose

long non-coding RNA BANCR knock out

Depositor

Xiaojun Lian

Publication

Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BANCR Exon1 gRNA (BANCR Human)

Use

CRISPR

Expression

Mammalian

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOC1 Gria1-GFP-FRB KI

Plasmid

#183428

Purpose

CreOFF knock-in for GluA1-GFP-FRB (amino acid position: stop codon)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and GFP-FRB donor

Use

CRISPR

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC2 Dlg4-Halo-FKBP KI

Plasmid

#183435

Purpose

CreOFF knock-in for PSD95-Halo-FKBP (amino acid position: R721)

Depositor

Harold MacGillavry

Publication

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and Halo-FKBP donor

Use

CRISPR

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Rac-WT(guide resistant)-2a-mcherry-U6ac-Rac guides

Plasmid

#168242

Purpose

"Rescue Rac expression in neutrophil specific knockout"

Depositor

Qing Deng

Publication

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Rac(guide resistant)-WT

Use

Zebrafish expression

Tags

mcherry

Promoter

LyzC

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G6_T804N_MTOR_E2419K_Dual_pegRNA

Plasmid

#178110

Purpose

Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-E2419K pegRNAs from two independent U6 promoters.

Depositor

Yannick Doyon

Publication

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ATP1A1 G6 T804N pegRNA + MTOR-E2419K pegRNA

Use

CRISPR; Prime editing

Expression

Mammalian

Promoter

Tandem U6 promoters

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Cacnb1 KI

Plasmid

#139660

Purpose

Endogenous tagging of CaVβ1: N-terminal (amino acid position: S8)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pPN461

Plasmid

#137877

Purpose

PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRi; mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Publication

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

POGZ (POGZ Human)

Use

CRISPR

Expression

Mammalian

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 C2CD4B-TSS-guide4

Plasmid

#125485

Purpose

CRISPR-mediated activation of C2CD4B. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Publication

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2 C2CD4A/B-enh2-guide2

Plasmid

#125470

Purpose

CRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7163757 (C2CD4A/B GWAS locus)

Depositor

Jorge Ferrer

Publication

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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Empty Bridge Control Zfp462-Klf4SE

Plasmid

#127666

Purpose

Encodes for 4 gRNAs expressed from their individual hU6 promoters. Two gRNAs target the Zfp462 promoter while the other two target the Klf4 stretch enhancer.

Depositor

Jennifer Phillips-Cremins

Publication

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

gRNAs 129, 135, 115, 117

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-Rosa26 gRNA (SpyCas9 scaffold)

Plasmid

#120296

Purpose

AAV vector; encodes GFP as well as a U6-driven Rosa26-targeting gRNA (SpyCas9 scaffold)

Depositor

Dominik Niopek

Publication

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

Rosa26 gRNA (SpCas9 scaffold)

Use

AAV and CRISPR

Expression

Mammalian

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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