We narrowed to 5,621 results for: pcas

1. pCAGGS-FLAG-CaMKIID

   Plasmid

   #186827

   Purpose

   Expresses FLAG-CaMKIID in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   Camk2d (Camk2d Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-ChrimsonR-tdT

   Plasmid

   #59169

   Purpose

   Expression vector for ChrimsonR-tdTomato

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   K176R

   Promoter

   CAG

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRha-ABE8e-SpCas9-NG

   Plasmid

   #201190

   Purpose

   Expresses the base editor ABE8e-SpCas9-NG in bacterial cells

   Depositor

   Jonathan Yen

   Article

   Mayuranathan et al Nat Genet. 2023 Jul;55(7):1210-1220. doi

   Insert

   ABE8e-SpCas9-NG
   

   Use

   Tags

   8xHIS and BP-NLS

   Expression

   Bacterial

   Mutation

   Promoter

   pRhaBAD

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG_Xph20-SNAPf-CCR5TC

   Plasmid

   #135537

   Purpose

   Encodes a specific PSD-95 binder (Xph20) fused to SNAPf, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph20
   

   Use

   Tags

   SNAPf

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG_Xph15-SNAPf-CCR5TC

   Plasmid

   #135536

   Purpose

   Encodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph15
   

   Use

   Tags

   SNAPf

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG2-EGFP-C-IFT140

   Plasmid

   #218739

   Purpose

   Expresses C-terminally EGFP-tagged IFT140 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Insert

   IFT140 (IFT140 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-eNpHR3.0-mRuby2-ST

   Plasmid

   #109134

   Purpose

   Halorhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   eNpHR3.0-mRuby2-ST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-nSpCas9-NoABE

   Plasmid

   #209043

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR_TRE_mcherry-P2A-Pcad

   Plasmid

   #133807

   Purpose

   inducible expression of mCherry and Pcad in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2018 Jul 13;361(6398):156-162.

   Insert

   cadherin 3 (CDH3)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-R2Tg-3xFlag

    Plasmid

    #223250

    Purpose

    Mammalian expression of C-terminally Flag-tagged R2Tg protein

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg protein
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-CoChR-mRuby2-ST

    Plasmid

    #109131

    Purpose

    Cation channelrhodopsin CoChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    CoChR-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9(BB)-2A-Puro-C9orf72

    Plasmid

    #83439

    Purpose

    Expresses Cas9 and a gRNA targeting C9orf72

    Depositor

    Shawn Ferguson

    Article

    Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

    Insert

    gRNA targeting human C9orf72 (C9orf72 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAX APP 751

    Plasmid

    #30138

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild type splice variant 751

    Promoter

    Available since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-ETF1-E55D

    Plasmid

    #130876

    Purpose

    Encodes ETF1 with an E55D substitution

    Depositor

    Prashant Mali

    Article

    Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

    Insert

    Eukaryotic transcritional termination factor ETF1-E55D (ETF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E55D

    Promoter

    CAG

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpCas9-NRCH-SsAPOBEC3B

    Plasmid

    #198550

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NRCH-SsAPOBEC3B-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-SsAPOBEC3B
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-HA-LMW-FGF2

    Plasmid

    #157663

    Purpose

    Expresses FGF2 in mammalian cell

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-134

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG/ATP9A(E195Q)-HA

    Plasmid

    #209244

    Purpose

    Mammalian expression of ATP9A mutant E195Q

    Depositor

    Hye-Won Shin

    Article

    Tone et al FEBS Lett. 2020 Feb;594(3):412-423. doi:

    Insert

    ATP9A (ATP9A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E195Q

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX459_gRNA pAS dual_hspCas9

    Plasmid

    #193312

    Purpose

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    not applicable

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG2-EGFP-C-IFT121

    Plasmid

    #218736

    Purpose

    Expresses C-terminally EGFP-tagged IFT121 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    IFT121 (WDR35 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG2-EGFP-C-IFT139

    Plasmid

    #218738

    Purpose

    Expresses C-terminally EGFP-tagged IFT139 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    IFT139 (TTC21B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG-ChroME2f-mRuby2-ST

    Plasmid

    #170160

    Purpose

    Cation channelrhodopsin ChroME2f fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2f-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCambia2300 - ProRUBY:RUBY-Citrine

    Plasmid

    #128796

    Purpose

    Fluorescent reporter for subcellular localisation and expression analysis of RUBY

    Depositor

    George Haughn

    Article

    Sola et al Plant Cell. 2019 Apr;31(4):809-831. doi:

    Insert

    RUBY PARTICLES IN MUCILAGE (AT1G19900 Mustard Weed)
    

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    ProRUBY

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCAG-HiBiT-CD81

    Plasmid

    #162593

    Purpose

    N-terminally HiBiT-tagged human CD81 under CAG promoter

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

    Insert

    human CD81 (CD81 human, Human, Synthetic)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPB-spCas9-pNeo

    Plasmid

    #214120

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Promoter

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCAG2-EGFP-C-IFT122

    Plasmid

    #218737

    Purpose

    Expresses C-terminally EGFP-tagged IFT122 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    IFT122 (IFT122 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDNR-SpCas9-Cdt1

    Plasmid

    #80426

    Purpose

    Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

    Depositor

    Sara Howden, Melissa Little

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9-Cdt1
    

    Use

    CRISPR

    Tags

    Cdt1

    Expression

    Mutation

    codon optimized for human

    Promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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27. pU6-PspCas13b-GEMSgRNA2

    Plasmid

    #204768

    Purpose

    pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    guide RNA targeting GEMS m6A sensor mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAGGS-FLAG-CaMKIIB

    Plasmid

    #186826

    Purpose

    Expresses FLAG-CaMKIIB in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    Camk2b (Camk2b Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAGGS-ChR2-Venus

    Plasmid

    #15753

    Purpose

    Expresses ChR2-Venus for neuronal excitation.

    Depositor

    Karel Svoboda

    Article

    Petreanu et al Nat Neurosci. 2007 May . 10(5):663-8.

    Insert

    Channelrhodopsin2-Venus
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AW01_pU.CAG.eSpCas9(1.1).rBGpA

    Plasmid

    #199255

    Purpose

    Expression construct encoding the high specificity eSpCas9(1.1) nuclease

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

    Insert

    High specificity eSpCas9(1.1) nuclease
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    K848A K1003A R1060A

    Promoter

    CAG promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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